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Volume 34 (1979)
- Issue 6 Pages 779-
- Issue 5 Pages 713-
- Issue 4 Pages 631-
- Issue 3 Pages 477-
- Issue 2 Pages 357-
- Issue 1 Pages 31-
Volume 34, Issue 6
Displaying 1-9 of 9 articles from this issue
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Morio HOMMA, Masato TASHIRO1979 Volume 34 Issue 6 Pages 779-795
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (7697K) -
Eiko HIBI1979 Volume 34 Issue 6 Pages 797-812
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe growth of Staphylococcus aureus 209P L-form was studied on solid and liquid media. Two basic culture media, BHI and semidefined medium containing vitamin-free casamino acids, were used with various concentrations of NaCl, NH4Cl or sucrose as osmotic stabilizer.
The results obtained are summarized as follows.
1. Growth on solid media.
L-form organisms grew on BHI medium, which contained 2.0-10.5% NaCl and 1.4-9.1% NH4Cl. Log of CFU increased directly in proportion to the concentrations of NaCl and NH4Cl.
They grew also on semidefined medium which contained 0.5-10.5% NaCl and 0.46-4.6% NH4Cl. The maximum number of colonies grown on this medium was obtained when 3.5-4.5% NaCl and 3.2% NH4Cl were contained in the medium.
L-form organisms grew on BHI medium containing 10-60% sucrose and no semidefined medium containing 5-60% sucrose. The maximum number of colonies was grown on both media containing 30% sucrose.
The size of colonies was maximum and the nipple/colony ratio minimum on BHI and semidefined media containing 3.5% NaCl and 30% sucrose.
2. Growth in liquid media.
L-form organisms existed in BHI medium containing 2.0-15.0% NaCl and 0.91-18.2% NH4Cl over four weeks. On the contrary, they were extinguished within two or three weeks from all the semidefined media used, except that containing 1.0-2.5% NaCl and 0.46-3.2% NH4Cl.
They survived both in BHI and semidefined media containing 10, 15 or 20% sucrose over three weeks.View full abstractDownload PDF (8301K) -
Yoshiharu YAMASAKI, Kenzo FUNAI, Motoo UESHIMA, Heizo SHINGU1979 Volume 34 Issue 6 Pages 813-825
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSEconazole nitrate and its related compounds were examined for activity in vitro against Candida albicans, Trichophyton mentagrophytes and Staphylococcus aureus by using the agar dilution technique. Econazole nitrate, its optical isomers and econazole base showed virtually the same antifungal activity. Of 15 econazole-related compounds, including chemical intermediates and metabolites, only 2, 4-dichloro-2-chloroacetophenone was active against those fungal strains.
Econazole was also compared with mionnazole and clotrimazole with regard to activity in vitro against 46 strains of medically important fungi, including yeast-like fungi, dermatophytes and several other filamentous fungi. Data on minimum inhibitory concentration and minimum fungi cidal concentration revealed that there was no qualitative difference in antifungal property against yeast-like fungi among the three imidazole drugs. Econazole and miconazole showed a slightly stronger fungicidal activity against C. albicans than clotrimazole.
Of the three imidazoles, econazole showed the strongest activity against dermatophytes and other filamentous fungi.
In shaking cultures of C. albicans and T. rubrum, a decrease in number of viable fungi was induced more rapidly and more remarkably by econazole or miconazole than by clotrimazole.View full abstractDownload PDF (1363K) -
II. Disparity of Agglutination Titers of Patient's Sera between Antigens Prepared from the Maeno and Recently Isolated StrainsKachiko SEKIYA, Mitsuo YAMADA, Atsushi TAKAMIYA, Yasukiyo NAKASE1979 Volume 34 Issue 6 Pages 827-835
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSIn the recent outbreak of whooping cough in the Miura Peninsula district, the agglutination titers of 127 patient's sera were compared between antigens prepared from the recent strains (serotype 1-3-4) and those from the Maeno strain (serotype 1-2-3-4) that had been used so far.
Of 111 positive sera, 78 (70.3%) were higher in titer against the recent strains than the Maeno strain, 16 (14.4%) higher in titer against the Maeno strain, and 17 (15.3%) showed equal titers against both strains. Significant differences were found in sera obtained in the early stage of illness.
Agglutinin absorption tests were performed with anti-rabbit sera, patient's sera and factor sera, and stability tests of bacterial suspensions with various media.
The results of both tests showed that no antibody against a new factor of B. pertussis agglutinogen was in any serum, and that disparity was present due to the difference in quantity of factor 3.
Factor 3 was predominant in the K agglutinogens in most of the recent strains. On the other hand, a relatively small amount of this factor was found in the Maeno strain that possesses all the factors, 1 to 4.View full abstractDownload PDF (975K) -
Kenji OHTA, Yasuo KUDOH, Masaaki TSUNO, Senzo SAKAI, Tsutomu MARUYAMA, ...1979 Volume 34 Issue 6 Pages 837-846
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSBased on reversed passive hemagglutination, a sensitive and specific serological assay was developed for detection of enteropathogenic toxin produced by Vibrio parahaemolyticus. It was so sensitive as to make the detection of 1ng/ml of the toxin practicable.
By using the assay, vibrios isolated from various sources were examined for toxin-produ-cibility. Kanagawa phenomenon-positive isolates of human origin produced more than 1μg/ml of the toxin. Most of the Kanagawa-negative and Kanagawa-questionable isolates from fecal specimens of gastroenteritis patients produced a smaller amount of the toxin. Those obtained from seafish, shellfish or their cooking utensils did not produce any detectable amount of the toxin. Based on these findings, the role of the toxin was discussed in causing diarrhea in the case of gastroenteritis due to Kanagawa-negative Vibrio parahaemolyticus.View full abstractDownload PDF (4465K) -
Yukio USUI, Shin-ichi NARIKAWA, Yoshitoshi ICHIMAN, Kosaku YOSHIDA1979 Volume 34 Issue 6 Pages 847-851
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA compact-colony-forming active substance (CCFAS) was extracted from a strain of Staphylococcus epidermidis. It showed similar biological activity and reacted with a serumreacting factor to CCFAS obtained from strains of S. aureus.
With these substances, however, serological heterogeneity was demonstrated by the agar diffusion test. Biological examination revealed that CCFAS obtained from the strain of S. epidermidis contained glycerol, while this substance from strains of S. aureus contained ribitol.View full abstractDownload PDF (4386K) -
Hiroko ICHIMARU, Masahiro NAKAMURA1979 Volume 34 Issue 6 Pages 853-859
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA film-and spot-formation on solid media by Mycoplasma species has been known to be an important characteristic for the certain species.
During studies on an anti-mycoplasma substance (mycoplasmacin) which was derived from mycoplasmal cells, it was found that a film was produced by disrupted mycoplasma cells of Mycoplasma salivarium on the surface of PPLO agar medium. Therefore, conditions necessary for producing a film involving both mycoplasma cells and solid medium were studied.
The results obtained indicate that a film was produced on Noble agar containing no yeast extract but horse serum alone by ultrasonicated mycoplasma cells, as well as in cell free-extract. Furthermore, it was determined that the film production was due to an enzymatic reation between mycoplasma lipase, possibly phospholipase A, and phospholipid, i.e., lecithin.View full abstractDownload PDF (8273K) -
Mitsuo TOKORO, Kiichi GOTO, Fuzizo YAMADA, Yuzi TERADA1979 Volume 34 Issue 6 Pages 861-862
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (178K) -
1979 Volume 34 Issue 6 Pages 863-882
Published: November 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (3115K)
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