Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 33, Issue 4
Displaying 1-6 of 6 articles from this issue
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  • Related Problems Concerning Cultivation of Mycobacterium leprae and M. lepraemurium in Cell-free Medium
    Masahiro NAKAMURA
    1978 Volume 33 Issue 4 Pages 607-628
    Published: July 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.607
    JOURNAL FREE ACCESS
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  • Antibiotic Treatment of Experimental Tularemia in Mice with Special Reference to Strong Immunity Established after Treatment
    Saburo HYODO
    1978 Volume 33 Issue 4 Pages 629-635
    Published: July 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.629
    JOURNAL FREE ACCESS
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    A study was made on the antibiotic treatment of experimental tularemia in mice. The antibiotics tested were streptomycin, oxytetracycline, tetracycline, kanamycin, chloramphenicol, leucomycin, and erythromycin. Mice (ddK) were inoculated subcutaneously with 104 MLD of the virulent strain (Ebina) of Francisella tularensis. The antibiotic treatment started 48 hours after inoculation. It was subcutaneous injection with a given dose of drug every 6 hours for 10 days. It presented the following results: (1) The antibiotics tested were all effective when administered in sufficient doses. (2) The therapeutic efficacy of the antibiotics appeared to have little relation with their permeability into cells, but to be almost proportionate to their bacteristatic activity in vitro. This result would be in conflict with the opinion that F. tularensis is an intracellular parasite. (3) The drug sensitivity and virulence of the organisms isolated from survived mice were equivalent to those of the original Ebina strain. (4) After the antibiotic treatment of tularemia, a strong immunity was established in survived mice. These mice could defend themselves against as large a challenge dose as 109 MLD of the virulent strain. (5) Immunization with formalin-killed organisms of the virulent strain and with living organisms of the avirulent strain gave rise to such a strong protective immunity in mice as comparable to that established by immunization with living virulent organisms through antibiotic therapy.
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  • The Effect of HgCl2 on the Germination of Bacillus megaterium QM B1551 Spores
    Tomihiko KOSHIKAWA, Masayoshi IMAGAWA, Kazuhito WATABE, Takamitsu SHIM ...
    1978 Volume 33 Issue 4 Pages 637-642
    Published: July 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.637
    JOURNAL FREE ACCESS
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    HgCl2 was studied for effect on the germination of Bacillus megaterium QM B1551 spores.The germination of spores by glucose was completely inhibited by 1mM of HgCl2. From such spores 46μg of Hg was detected.
    The germination of HgCl2-treated spores occurred when the spores were resuspended in nutrient broth and incubated. The mercury contained in these spores was released in the course of germination.
    The extraction of spores with 8M urea caused the release of 101μg of protein per mg of spores. When treated with HgCl2, spores retained 26.2μg of Hg per mg of spores after treatment.
    There were small differences in reactions to germination agents between the HgCl2-treated and non-treated spores.
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  • Salt-Tolerance Mechanism of Staphylococcus aureus
    Role of Proline and Water in Osmoregulation of S. aureus
    Izumi KOUJIMA
    1978 Volume 33 Issue 4 Pages 643-649
    Published: July 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.643
    JOURNAL FREE ACCESS
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    To elucidate the salt tolerance mechanism of Staphylococcus aureus, the adaptive changes of the contents of amino acids and water in the cell were studied by altering the salt concen-tration of the growth media.
    Only a trace amount of proline was detected in the cells of S. aureus grown in a medium containing 0.1M NaCl. The cells grown in a medium containing 1.0 and 1.8M NaCl had 428 and 1, 440μmoles proline accumulated, respectively, per g of dry weight of cells. The intracellular water content was 1.70g/g dry wt. when cells were grown in a medium containing 0.1M NaCl. When the cells were transferred to a medium containing 1.8M NaCl, their water content decreased to 0.80g/g dry wt. within one minute, while their intracellular free proline content increased gradually from O to 1, 400μmoles/g dry wt. after incubation at 37C for 30 minutes. Meanwhile their water content increased to 0.88g/g dry wt. The accumulation of proline was found to be due to a temperature-dependent selective uptake from the medium. The accumulated proline was released quickly when the cells were transferred from a high salt medium to a low salt solution. The efflux of proline occurred at 0-4C, suggesting that the process might not be energy-dependent.
    The role of proline and water content in the osmoregulation mechanism of S. aureus was made clear. The salt-tolerance mechanism was discussed, taking its correlation with adaptive changes in the cell-membrane constituents into consideration.
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  • [title in Japanese]
    1978 Volume 33 Issue 4 Pages 651-652
    Published: July 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.651
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1978 Volume 33 Issue 4 Pages 653-662
    Published: July 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.653
    JOURNAL FREE ACCESS
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