Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 41, Issue 3
Displaying 1-9 of 9 articles from this issue
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  • Aminoglycoside Antibiotics
    Mechanisms of Action and Resistance
    Nobuo TANAKA
    1986 Volume 41 Issue 3 Pages 587-601
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.587
    JOURNAL FREE ACCESS
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  • Requirement of cyclic 3', 5'-monophosphate in the production of a permeability increasing factor by Vibrio cholerae
    Teruyo ITO, Takeshi YOKOTA, Shigeru MATSUSHITA, Kenji OHTA, Yasuo KUDO ...
    1986 Volume 41 Issue 3 Pages 603-610
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.603
    JOURNAL FREE ACCESS
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    Enterotoxin production by cAMP-deficient mutants of Vibrio cholerae was investigated by the latex agglutination test, CHO cell elongation system, and permeability increasing test. It was revealed that two kinds of permeability-increasing factors were produced by V. cholerae E2 and its cAMP-deficient mutant E2751. They were cholera toxin (CT) and an unknown permeability-increasing factor (PF). PF was separated from CT by DEAE-Sephacel column chromatograhy. Antiserum was prepared against partially purified PF separated by DEAE-Sephacel chromatography and used for the neutralization tests. Neutralization tests with anti CT antiserum and anti PF antiserum showed that CT and PF were entirely different immunplogically. We showed also that though cAMP is not essential for production of CT, it is required for production of this newly found PF.
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  • Pathogenic factors in mixed infection due to Bacteroides fragilis and other species of bacteria in mice
    Shuichi MIYAZAKI, Tetsuo ISHII, Akiyoshi TSUJI, Susumu KITAYA, Yasuko ...
    1986 Volume 41 Issue 3 Pages 611-617
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.611
    JOURNAL FREE ACCESS
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    The present study was undertaken to see whether the virulence of Bacteroides fragilis is enhanced by mixed infection with Escherichia coli.
    1. Acceleration of growth of B. fragilis in mixed culture
    B. fragilis GM7004 (102CFU/ml) inoculated alone into nutrient broth did not grow at 37C for 24hrs. When cultured under the same conditions with 102CFU/ml of E. coli No.94, the bacterial cell population increased to 108CFU/ml or more. This increase was attributed to consumption of the medium-dissolved oxygen by proliferating E. coli and a decreased oxidation-reduction (O-R) potential. B. fragilis GM7004 did not grow in the presence of Pseudomonas aeruginosa E7 and, in this case, O-R potential did not decline though the dissolved oxygen was consumed.
    Furthermore, O-R potential of both intraperitoneal fluid and serum declined with time when E. coli No.94 was used for the mixed infection with B. fragilis GM7004.
    2. Enhancement of virulence of B. fragilis in mixed infection
    When mice were challenged intraperitoneally with a combination of B. fragilis GM7004 and E. coli No.94 or P. aeruginosa E7, both of which have high superoxide dismutase (SOD) and catalase activities, the virulence of B. fragilis was markedly enhanced in both experiments.
    On the other hand, the mixed infection with B. fragilis GM7004 and E. coli JC-2 or P. aeruginosa IFO3445, both of which have low SOD and catalase activities, did not enhance the virulence under the same conditions.
    From the results, it is considered that the pathogenicity of B. fragilis is enhanced in the mixed infection with other species which have the capacity of declining O-R potential in the infected site and high SOD and catalase activities.
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  • Quantitative method for live bacteria in murine peritoneal macrophages by solubilizing with Triton X-100
    Haruo MATSUMURA, Yasuhiko TERADA, Masayasu NAKANO
    1986 Volume 41 Issue 3 Pages 619-621
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.619
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1986 Volume 41 Issue 3 Pages 623-630
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.623
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1986 Volume 41 Issue 3 Pages 631-640
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.631
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1986 Volume 41 Issue 3 Pages 641-661
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.641
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1986 Volume 41 Issue 3 Pages 662-671
    Published: May 25, 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.662
    JOURNAL FREE ACCESS
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  • Erratum
    1986 Volume 41 Issue 3 Pages 676
    Published: 1986
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.41.3_676
    JOURNAL FREE ACCESS
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