All issues
Volume 17, Issue 8
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
-
V. Studies on Duration of the maintenance of Infectious Activity of Myc. Lepraemurium Under Anaerobic ConditionMasahiro NAKAMURA1962 Volume 17 Issue 8 Pages 679-682
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA comparision of the maintenance of infectious activity of Myc. lepraemurium kept under aerobic and anaerobic conditions was investigated. The Hawaiian strain of Myc. lepraemurium suspension in M/20 Sorensen buffer (pH 6.9) with and without 0.5% glucose or pyruvate was kept under aerobic or anaerobic condition which was made by adding paraffin oil. The materials for testing infectivity were taken from each tube stored under definite conditions at appropriate times. The materials were subcutaneously inoculated into several mice for each condition, and mice tested were killed about five months after inoculation.
The results obtained here demonstrated that the infectious activity of Myc. lepraemurium was kept for more than 10 days if the bacillary suspension was stored under anaerobic condition, on the contrary, if the suspension was kept under aerobic condition, the infectious activity of the bacilli was lost within 7 days' incubation. The similar findings were observed in the cases of media containing glucose or pyruvate. However, when the medium contained glucose, the infectious activity of the bacillus was lost for a short time even if kept under anaerobic condition. On the other hand, if the medium contained pyruvate, the infectious activity of the bacillus was maintained for more than 20 days after incubation at 37°C either under aerobic and anaerobic condition.View full abstractDownload PDF (671K) -
Shichiro NISHIMURA1962 Volume 17 Issue 8 Pages 683-686
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSSalmonella pullorum strain No.21 could not grow even in the semisynthetic medium containing eight vitamins. The addition of three purine derivatives to the casamino acids-medium was very effective for the growth of this strain, but, pyrimidine derivatives showed no growth-acceleration.
In the purines, adenine and guanine were effective over the level of 2.5-5.0 γ/ml, xanthine was not active. In the Yasushi's or modified Yasushi's medium containing 10 γ/ml of adenine, strain No.21 showed good growth. By the elimination test, it was confirmed that this strain required proline, glutamic acid and cystine for its growth, and niacin, thiamine and riboflavin were also helpful for this strain.View full abstractDownload PDF (563K) -
Morimasa KASUYA1962 Volume 17 Issue 8 Pages 687-694
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe transfer of the drug resistance of enteric bacteria in the intestine of germfree and conventionalmice was studied with Shigellae, Escherichiae, and Klebsiellae.
Using mostly the technique described by Freter (1956), these organisms from a 24 hour broth culturewere given to the mice by stomach tube. Of course, antibiotics water were not given throughout the courseof the experiment after challenge of drug sensitive bacteria.
Results obtained are as follows;
1) All of the resistance factors (Streptomycine, Chloromycetin, Tetracycline, and Sulfathiazole) wereeasily transferred, when donor and recipient multiplied together in the mouse intestine.
2) Transferred resistance was further retransferred to sensitive enteric bacteria in the mouse intestine.
3) From four out of six experiments, resistance-transferred bacteria showed equal level and patternof resistance in each group. In two experiments, two kinds of resistance pattern were found in the transferredbacteria, but all transferred bacteria in each group showed the equality of resistance level.
4) From the experiment, the transferred resistance is exactly equal, when it is passed from onedonor to other genus-differing recipients, nevertheless, if the transmission occurs between donors from differentgenus and the same recipient, the transfer-result might differ, or otherwise, equal. Therefore, it seemsthat transferred resistance factors are rather determined by donor than recipient.
5) On the conclusion, it is also believed that the generation of the resistance in the human entericbacteria is due to the transmission of resistance against drug from one previously existing enteric bacteria tothe next-comer.View full abstractDownload PDF (1145K) -
II. Studies on the factors influencing phagocytosis of Mycobacteria by L cells, especially the effect of old tuberculin treatment to the cellsHiroyuki SHIKATA, Masahiro NAKAMURA1962 Volume 17 Issue 8 Pages 695-701
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe effects of pretreatment of BCG-old tuberculin (OT) to the L cells and the presence of BCG-oldtuberculin in the culture media on the infection of BCG to the cells were studied and the following resultswere obtained:
1. Pretreatment of BCG-old tuberculin to the cells stimulated phagocytosis of Mycobacteria by the Lcells. The phagocytosis rate was increased especially when 10-1 or 10-2 concentration of old tuberculin was.used for treatment to the cells which were cultivated for more than 48 hours.
2. It was demonstrated by the immunological method using skin reaction that BCG old-tuberculin.pretreated was bound to the cells, but it could not be found what kind of effect of tuberculin enhanced thephagocytosis of Mycobacteria by the L cells and that there was any immunological relationship betweentuberculin and bacilli in the case of phagocytosis.View full abstractDownload PDF (1138K) -
II. On the Appearance of Resistant Organisms in Shigella Infections of Mice with the Aid of Antibiotic AdministrationKazuo IWATA, Hiroko MOTOE1962 Volume 17 Issue 8 Pages 702-705
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn a previous study the authors reported that Shigilla infection was experimentally induced in miceby oral inoculation of resistant strains of Shigella. This was accomplished by continuous administration ofantibiotics in combination with pre- and post-inoculation of resistance-lowering agents, such as carbon tetrachloride, which were very useful in the establishment of infection. In this connection the authors showedthat in such an infection, the presence of an increase number of antibiotic-resistant organisms, mainly Escherichia coli, due to the effect of chloramphenicol, disrupts the Shigella population.View full abstractDownload PDF (645K) -
IV. Virulence of Fat-Coated Saprophytic Add-Fast Bacteria on the Rabbits and Mice Virulence of Oil-Coated INH-Resistant Tubercle BacilliSukeyoshi KUDOH1962 Volume 17 Issue 8 Pages 706-710
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn the previous reports, it was repeatedly confirmed that M. phlei coated with the fat extracted fromguinea pigs gave the severe changes on the guinea pigs, while the water-suspended one showed no visiblelesions.
This paper presents that the similar phenomena are observed also in the rabbits and mice.
Both animals were inoculated intracerebrally and intraperitoneally with M. phlei coated with fatextracted from each animal.
Rabbits inoculated intracerebrally with fat-coated bacilli revealed the wide-spread meningeal abscess, and intraperitoneally showed severe adhesions in abdominal cavity with numerous abscesses and tubercles insome organs.
In the case of mice, the similar findings were observed, but various organs had no tubercle.
M. phlei was detectable for a longer time in both animals inoculated with fat-coated bacilli, whilenone or few in the water-suspended one.
Furthermore, the attenuated strain of INH-resistant tubercle bacilli was injected on the guinea pigsunder the condition of coating with liquid paraffin. The results of this trial showed the virulence of thisstrain was preserved by mean of Oil-coating same as M. phlei.View full abstractDownload PDF (4307K) -
Takahiro HORIKAWA, Takeshi SUZUKI, Hiroshi FUJITA, Kiyoshi YAMADA, Hid ...1962 Volume 17 Issue 8 Pages 711-722
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSRecent trend of popular application of antibiotics has resulted suppression of incidence of pathogenicorganisms in many cases which were often replaced by Ps. aeruginosa because of its in difference from theseantibiotics. Authors report the results of investigations carried on biochemical and serological characteristicson this species and additionally the transfer of resistance against several antibiotics from a resistant strain toa sensitive one.View full abstractDownload PDF (2062K) -
I. The rates of adsorption, abortion and propagation of mycophage in the 12 bacterium-phage systems and the effect of Tween 80 on the phage adsorptionTohru TOKUNAGA, Yasuo MIZUGUCHI, Yoneo MARUYAMA, Toyoho MUROHASHI1962 Volume 17 Issue 8 Pages 723-727
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSQuantitative studies were made on the EOP, adsorption rate, abortion rate, adsorption velocity constantand propagation rate, using 3 mycophages and 4 mycobacterial strains.
The adsorption velocity of mycophage was found to be rather slow, and the velocity constant calculatedwas in the order of 10-10-10-11.
Under the present experimental conditions, only two among 12 systems have shown the adsorptionrates of more than 90% after 60 minutes adsorption.
Rather high abortion rate was found in a few systems, especially in D 29-Myc. Phlei-yoken system.Namely, in this system, all of the phage particles which adsorbed to nearly 90% to bacterial cells wereabortive.
Generally, the system, in which the difference between adsorption and abortion rates was big, seemedto have a good efficiency of phage propagation. For instance, the system of HC-Myc. sp. jucho had thelargest propagation rate, in spite of its relatively low adsorption rate.
Tween 80 inhibited the adsorption and the propagation of mycobacteriophage in all 12 systems.View full abstractDownload PDF (848K) -
II. Isolation of the Acid-Precipitating Substance and its AntigenicityShizuo TAKAGI, Takeshi HIRONAO1962 Volume 17 Issue 8 Pages 728-732
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn the preceding study, the acid-precipitating substance (A-P substance) of B. megateriun precipitatedneither with homologous antiserum nor with heterologous antiserum and that of B. anthracis did notprecipitate with heterologous antiserum. As one of the reason, the lower concentrations of these A-Psubstances in test solutions were considered. In this study, therefore, the A-P substances were prepared inlarge amounts and relatively concentrated solutions of these A-P substances were used. As was expected, the A-P substance from each organism exhibited not only the specific antigenicity but also the commonantigenicity. However, the titers against homologous antiserum were higher than those against heterologousantiserum. The tests performed in agar gel exhibited the presence of considerable amounts of specific antigenin the A-P substance of each organism. These results suggest that the specific antigen components containedin the culture fluid can be concentrated by acid-precipitation.
Antigenicity of the A-P substance of each organism was hardly influenced by heat (100°C, 20 min.) orby trypsin digestion. These results suggest that the A-P substance contains thermostable, nontrypsin-digestibleantigen components.View full abstractDownload PDF (825K) -
Isolation of special type of R factor that is easily segregated and eliminated from host strainsMasato MORIMURA, Eizaburo TEJIMA, Kohkichi SAKAMOTO, Hajime HASHIMOTO, ...1962 Volume 17 Issue 8 Pages 733-737
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSFrom the field survey of drug-resistant E. coli, it was found that there are carriers of several patternsof drug-resistant E. coli. The RMF2 TC. CM. SM. SA and RMF25 (TC. CM. SM. SA) factors werefound, that were transferable by conjugation and able to transfer the drug-resistance to four drugs; tetracyclineTC', chloramphenicol (CMI, streptomycin (SM) and sulfanilamide (SA). These factors were eliminatedspontaneously from a host strain in high frequency. From the segregation of these factors, R (CM SM. SA), R SM. SA, and R, -1C. SA) factors were found and not transferable by conjugation, whereas RMF2 and RMF25 factors were transferable by conjugation.View full abstractDownload PDF (2113K) -
1962 Volume 17 Issue 8 Pages 738-754
Published: August 25, 1962
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (3517K)
- |<
- <
- 1
- >
- >|