Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 33, Issue 6
Displaying 1-7 of 7 articles from this issue
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  • The Structural Basis of Outer Membrane Permeability in Gram-Negative Bacteria
    Taiji NAKAE, Hiroshi NIKAIDO
    1978 Volume 33 Issue 6 Pages 715-727
    Published: November 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.715
    JOURNAL FREE ACCESS
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  • Plasmid-Determined Resistance to Heavy Metals
    Kazuo IZAKI
    1978 Volume 33 Issue 6 Pages 729-742
    Published: November 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.729
    JOURNAL FREE ACCESS
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  • Application of SP-Sephadex Chromatography to the Purification of Staphylococcal Enterotoxins A, B and C2
    Takahiro ODA
    1978 Volume 33 Issue 6 Pages 743-752
    Published: November 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.743
    JOURNAL FREE ACCESS
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    Essentially the same simplified methods were devised for the purification of staphylococcal enterotoxins A, B and C2. Each of them consisted of only two steps, chromatography on SP-Sephadex C-25 and gel filtration on Sephadex G-75. Each purified enterotoxin was shown as a band by disk electrophoresis at pH 4.3. It formed one precipitin line on agar against homologous anti-enterotoxin serum prepared from rabbits immunized with the purified enterotoxin. Each anti-enterotoxin serum formed one fused precipitin line against crude enterotoxin and reference toxin.
    Neither α-hemolysin nor β-hemolysin was detected from purified enterotoxin A, B, or C2.
    The rate of recovery by the purification steps ranged approximately from 73% to 85%.
    By SP-Sephadex C-25 chromatography, each enterotoxin was resolved into two or three fractions which were not distinguished immunologically from one another. Heterogeneity of the three enterotoxins was also observed by disk electrophoresis at pH 9.5 and isoelectric focusing in 7.5% polyacrylamide gels with 2% Ampholine, pH 3.5-10.0. From the results of isoelectric focusing in gels, it was recognized that enterotoxin A had at least 5 components with an isoelectric point of approximately 6.5, 6.8, 7.2, 7.7, and 8.3, respectively, enterotoxin B at least 7 components with an isoelectric point of approximately 8.2, 8.5, 8.7, 8.9, 9.0, 9.1, and 9.3, and enterotoxin C2 at least 4 components with an isoelectric point of approximately 6.2, 6.8, 7.3, and 7.6.
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  • Enumeration of Bifidobacteria in Dairy Products
    Susumu TERAGUCHI, Mariko UEHARA, Katsuhiro OGASA, Tomotari MITSUOKA
    1978 Volume 33 Issue 6 Pages 753-761
    Published: November 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.753
    JOURNAL FREE ACCESS
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    An improved selective medium, neomycin-paromomycin-nalidixic acid-lithium chloride (NPNL) agar, was developed for the enumeration of bifidobacteria in dairy products containing bifidobacteria, lactobacilli and streptococci. NPNL agar was BL agar without blood containing neomycin sulfate (100μg/ml), paromomycin sulfate (200μg/ml), nalidixic acid (15μg/ml), and lithium chloride (3mg/ml). It was employed as a plating medium. Incubation was carried out at 37C for 2 days in an anaerobic steel-wool jar filled with an atmosphere of 100% CO2.
    In the case of dispensation with anaerobic jars, modified NPNL agar in combination with the shake culture tube method with an additional agar medium overlaid was also proved to be useful for the same purpose. Modified NPNL agar was BL agar without blood containing neomycin sulfate (30μg/ml), paromomycin sulfate (50μg/ml), nalidixic acid (15μg/ml), and lithium chloride (3mg/ml).
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  • Adjuvant Activity and Antigenicity of MAF3 Determined by the Lymphocyte Blastogenesis
    Yasuhiro KAWAMURA, Yoshio KUMAZAWA, Tada-aki OKADA, Mikito MORISHITA, ...
    1978 Volume 33 Issue 6 Pages 763-765
    Published: November 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.763
    JOURNAL FREE ACCESS
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  • Colonization of Pseudomonas aeruginosa in the Digesitve Truct of Limited-Flora Mice
    Toru URANO, Kazuyoshi MAEJIMA, Kikuji ITO, Tomotari MITSUOKA
    1978 Volume 33 Issue 6 Pages 767-769
    Published: November 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.767
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1978 Volume 33 Issue 6 Pages 771-788
    Published: November 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.771
    JOURNAL FREE ACCESS
    Download PDF (2942K)
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