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Volume 28 (1973)
- Issue 6 Pages 489-
- Issue 5 Pages 401-
- Issue 4 Pages 357-
- Issue 3 Pages 315-
- Issue 2 Pages 213-
- Issue 1 Pages 22-
Volume 28, Issue 5
Displaying 1-4 of 4 articles from this issue
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Naoki NISHIDA1973 Volume 28 Issue 5 Pages 401-411
Published: September 25, 1973
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1650K) -
2, Experimental Infection in MonkeysTsutomu MARUYAMA1973 Volume 28 Issue 5 Pages 413-421
Published: September 25, 1973
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA series of experiments were performed, to cause infection of Yersinia enterocolitica in monkeys using the Ye ZM 4 strain (O antigen type 3) which had originally been isolated from a human autopsy case in this laboratory. In them, 5×109 organisms were administered orally into each of 10 cynomolgus monkeys (Macaca irus) weighing about 1-2kg. Two animals each were sacrificed on the 1st, 2nd, 5th, 10th and 20th day after the administration.
None of the infected animals had manifested any noteworthy clinical symptom until they were sacrificed, except three which had suffered from watery diarrhea and one of which excreted bloody mucus.
The organism was successfully recoverd from the contents of the ileum, jejunum, and colon of all the animals killed on the 1st to 10th day.
The common histopathological changes of the intestines were a marked proliferation of reticular cells and histiocytes and infiltration of neutrophils.
The mesenteric lymph nodes examined showed mild but clear pathological changes. The organism was recovered from all the monkeys, except three killed on the 2nd, 5th, and 20th day, respectively. None of the monkeys developed septicemia, although the organism was detected from two of 10 splenic specimens collected on the 5th and 10th day, respectively.
When the animals were examined for the specific antibody response, the highest titer of anti-O agglutinin, or 1: 1280, was observed in their sera obtained on the 5th day.View full abstractDownload PDF (5902K) -
1. Isolation and IdentificationKeiu NINOMIYA1973 Volume 28 Issue 5 Pages 423-431
Published: September 25, 1973
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDuring four years from 1968 to 1972, 275 strains of anaerobic cocci were isolated from 260 clinical specimens, including pus, vaginal discharge, sputum, pleural fluid, urine, and urethral discharge. For the isolation of anaerobic cocci, GAM (Nissui), phenylethyl alcohol agar (BBL) with 5% sheep blood, and liver veal agar (Difco) with 5% sheep blood had been used. Subcultures of the isolated strainis were made in semisolid GAM medium (Nissui) and served for identification according to Prévot's Manual (1966). For the examination of biochemical properties, thioglycollate medium (Difco: without glucose and indicator) was used as basic medium, except for the tests of gas production, milk coagulation and gelatin liquefaction.
The isolated anaerobic cocci consisted of Peptococcus asaccharolyticus (55 strains), P. prevotti (29 strains), P. variabilis (25 strains), P. anaerobius (8 strains), Peptostreptococcus anaerobius (35 strains) including Ps. putridius, Ps. magnus (8 strains), Ps. foetidus (7 strains), Ps. intermedius (5 strains), and Veillonella alcalescens (19 strains). Forty-eight strains of gram-positive anaerobic cocci however, could not be identified as any of the speices described in Prévot's Manual, Bergey's Manual (1957), or Beerens' Manual (1966).View full abstractDownload PDF (865K) -
1973 Volume 28 Issue 5 Pages 435-466
Published: September 25, 1973
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (4635K)
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