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Volume 31 (1976)
- Issue 6 Pages 663-
- Issue 5 Pages 593-
- Issue 4 Pages 535-
- Issue 3 Pages 357-
- Issue 2 Pages 285-
- Issue 1 Pages 27-
Volume 31, Issue 6
Displaying 1-5 of 5 articles from this issue
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[in Japanese]1976 Volume 31 Issue 6 Pages 663-680
Published: November 25, 1976
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe anaerobist working on bibliography is surely a creature to be pitied and is not to be blamed for failing to follow the work of others conscientiously and thoroughly.View full abstractDownload PDF (5527K) -
[in Japanese]1976 Volume 31 Issue 6 Pages 681-694
Published: November 25, 1976
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (8307K) -
Shiro IUCHI, Shuji TANAKA1976 Volume 31 Issue 6 Pages 695-704
Published: November 25, 1976
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSSynthesis of L-arabinose isomerase (L-arabinose ketol isomerase, EC 5. 3. 1. 4) was studied in the wild-type and mutant strains of Vibrio parahaemolyticus. Two mutant strains, 2001 and 2126, used in this study were described previously (Iuchi et al.: J. Bacteriol. 124, 567-569, 1975). Strain 2001 is probably equivalent to the crp mutant lacking cyclic adenosine 3', 5'-monophosphate (cyclic AMP) receptor protein (CRP) of Escherichia coli. Strain 2126 is phenotypically similar to the cya mutant of E. coli deficient in adenylate cyclase, but the exact nature of this mutant is yet unknown.
L-Arabinose isomerase of V. parahaemolyticus was an inducible enzyme, and its activity was detected only when L-arabinose was present in the medium. The enzyme synthesis in the mutants was greatly impaired, and the specific activity obtained with each mutant was about 30% of that obtained with the wild-type strain. Cyclic AMP added to the medium could restore L-arabinose isomerase synthesis in strain 2126, indicating that the nucleotide, as well as the inducer L-arabinose, was required for synthesis of the enzyme. Glucose and galactose present in the medium repressed the enzyme synthesis of the wild-type strain by about 90 and 55%, respectively. No repression was observed with glycerol. All these findings suggest that Larabinose isomerase of V. parahaemolyticus is a typical catabolic enzyme, whose synthesis is regulated in the same manner as that of various catabolic enzymes of E. coli.View full abstractDownload PDF (1692K) -
Asako FUJISAWA, Yoneo KUBOTA, Shuji TANAKA1976 Volume 31 Issue 6 Pages 705-712
Published: November 25, 1976
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSStrain 1050, a mutant of Vibrio parahaemolyticus lacking a component of the phosphoenolpyruvate: sugar phosphotransferase system (PTS), did not utilize glucose and trehalose as the carbon and energy source. It was also defective either totally or partially in the utilization of a number of other carbon sources: mannose, mannitol, galactose, maltose, L-arabinose, ribose, glycerol, pyruvate, and succinate, but these defects could be overcome by adding cyclic adenosine 3', 5'-monophosphate (cyclic AMP) to the medium. Cyclic AMP did not restore the utilization of glucose and trehalose. Growth of the mutant on fructose was apparently normal, regardless of the presence of exogenous cyclic AMP.
Two different types of revertants were obtained from strain 1050, and their representatives were designated strains 1050R and 1050A, respectively. The former strain seemed to be a true revertant, because PTS activity determined with methyl-α-D-glucoside as the substrate, as well as the utilization of all the carbon sources mentioned above, was restored in this strain. Strain 1050A was selected for its ability to metabolize galactose. It remained unable to phosphorylate methyl-α-D-glucoside. It failed to grow on glucose and trehalose, but grew normally on all the other carbon sources, including galactose. Therefore, the phenotype of strain 1050A was very similar to that of strain 1050 growing with exogenous cyclic AMP. Like the Escherichia coli mutants described earlier (Pastan and Perlman: J. Biol. Chem. 244, 5836-5842, 1969), strain 1050 would fail to produce certain catabolic enzymes. Such failure would have been “suppressed” in strain 1050A independently of the original PTS mutation.View full abstractDownload PDF (1536K) -
Yoshiaki ANDO1976 Volume 31 Issue 6 Pages 713-717
Published: November 25, 1976
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSAn attempt was made to elucidate the correlation between ion exchange properties and ionic germination in spores of Clostridium perfringens type A NCTC 8238. Further studies on the morphology were carried out with calcium, potassium, and hydrogen-loaded spores and for untreated native spores.
All the cationic form spores did germinate in the presence of added KCl or CaCl2 alone. Ca2+, however, inhibited the action of K+ to induce the germination of all the spores, but hydrogen form spores. Spot analysis was performed by scanning electron microscopy and electron probe X-ray microanalysis with an energy dispersive type X-ray microanalyzer. It revealed that non-exchangeable calcium was distributed mainly in the central region of the spore corresponding to the core, and that exchangeable calcium, as well as phosphorus, sulfur, and chlorine, was distributed throughout the spore.View full abstractDownload PDF (3405K)
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