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Volume 43 (1988)
- Issue 6 Pages 951-
- Issue 5 Pages 899-
- Issue 4 Pages 799-
- Issue 3 Pages 621-
- Issue 2 Pages 537-
- Issue 1 Pages 55-
Volume 43, Issue 5
Displaying 1-5 of 5 articles from this issue
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Kikuo NOMOTO1988 Volume 43 Issue 5 Pages 899-910
Published: September 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1709K) -
Ken-ichi AMANO, Sakae MIZUSHIRI, Kazue FUKUSHI1988 Volume 43 Issue 5 Pages 911-916
Published: September 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSLipopolysaccharides (LPSs) from rough-type Salmonella and E. coli migrated with mobilities in inverse proportion to the molecular weights in SDS-polyacrylamide gel electrophoresis (PAGE) in 17.5 to 20% of gel concentration. The whole cells of Chlamydia trachomatis and C. psittaci boiled and treated with proteinase K showed a single band of about 3, 700 daltons estimated by comparison with the molecular weights of LPSs of roughtype Salmonella. The LPSs extracted with phenol-water from Bordetella pertussis showed one band (about 4, 500 daltons) or two (approximately 4, 500 and 3, 700 daltons) depending on the strain. LPS from B. bronchiseptica migrated in two bands (about 5, 100 and 3, 500 daltons). Thus, we can presume the molecular weights of LPS from rough-type Chlamydia sp. and Bordetella sp. by SDS-PAGE.View full abstractDownload PDF (3313K) -
Ken-ichi SAKAMOTO, Shin-ichi MORINAGA, Takayoshi YAMAGISHI, Ken-ichi K ...1988 Volume 43 Issue 5 Pages 917-926
Published: September 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSTo clarify the fate of Clostridium perfringens in the intestines of the guinea pig, effects of the intestinal juice, bile acid and intestinal anaerobic bacteria on the growth of the organism were investigated. When vegetative cells and spores of C. perfringens were inoculated into guinea-pig intestinal juice medium sterilized by autoclaving, the bacterium proliferated and showed good spore formation. In non-sterilized intestinal juice medium or its supernatant, however, especially that with colon material, C. perfringens was unable to survive and form spores. The number of spores in this medium was reduced to less than 1/10 to 1/100 of that inoculated. On the other hand, when vegetative cells of C. perfringens were inoculated into Millipore-filtered intestinal juice medium, two contrast results with regard to growth and survival were obtained. These results indicate that a certain heat-labile substances which are able to inhibit the proliferation and colonization of invading bacteria in the intestinal tract may be present in the intestinal juice. Bile acid inhibited the increase of anaerobic bacteria in the following order of strength: Clostridium>Bifidobacterium≥Bacteroides>Peptostreptococcus. Furthermore, the growth of C. perfringens was inhibited by grampositive anaerobic bacteria and Bacteroides, but not by Fusobacterium.View full abstractDownload PDF (1043K) -
Kazuhito WATABE, Kyoko MAEKAWA, Atsumi YAMADA, Tomihiko KOSHIKAWA, Set ...1988 Volume 43 Issue 5 Pages 927-930
Published: September 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA method is described for analysis for dipicolinic acid (DPA), a bacterial spore-specific material, by high-pressure liquid chromatography using an anion exchange column and a mixture of EDTA (pH 3.4) and acetonitrile (20:1) as the mobile phase. The method provided a linear response to DPA concentration over a range of 0.2 to 40ng, and the response was not interfered with by any of the DPA analogs tested or non-sporulating bacterial cell lysate. The quantities of DPA determined by this method in many spores and sporulating cells of Bacillus agreed well with those determined previously by the colorimetric method.View full abstractDownload PDF (289K) -
1988 Volume 43 Issue 5 Pages 931-935
Published: September 25, 1988
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (608K)
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