Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
Volume 29, Issue 5
Displaying 1-4 of 4 articles from this issue
  • [in Japanese]
    1974 Volume 29 Issue 5 Pages 729-741
    Published: September 25, 1974
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese]
    1974 Volume 29 Issue 5 Pages 743-747
    Published: September 25, 1974
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    Download PDF (867K)
  • Seika MARUYAMA, Takashi TSURUMIZU, Seiji MATSUI
    1974 Volume 29 Issue 5 Pages 749-755
    Published: September 25, 1974
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    1. Seventy-two of 82 infant rabbits born from 12 rabbits immunized with killed Vibrio cholerae (NIH 41 Ogawa) were inoculated with live V. cholerae by Dutta's method (Dutta test). Fifty of them survived and were subjected to the aggulutination test. As a result, 31 rabbits were proved to be carriers, and three of them discharged the bacteria for 2 weeks. Two to 3 months after the Dutta test the intestinal loop test (De test) was performed on the rabbits of both carrier and noncarrier groups. The carrier group developed “crisis” less frequently than the non-carrier group. The serum antibody titer remained at a low level over an interval of time between Dutta and De tests, irrespective of the carrier state.
    2. Ten of the 82 infant rabbits underwent the De test without the preceding Dutta test when they were 2 to 3 months old. The contents of intestinal loops of rabbits positive for the De test were all positive for this test on other rabbits. No vascular permeability factor was detected from them.
    3. Histopathologically, rabbits strongly positive for the De test using t he live bacteria exhibited necrotic, desquamative, and suppurative changes in the intestine. In contrast, no suppurative changes were observed in the intestine of rabbits strongly positive for the De test using cell-free cholera enterotoxin. The intestinal tract of these rabbits was remarkably distended, with its wall extremely flattened.
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  • II. Serological Relationships among Galactomannans from Three Species of the Genus Hormodendrum, H. pedrosoi, H. compactum and H. dermatitidis
    Noriyuki TAKEDA, Shigeo SUZUKI
    1974 Volume 29 Issue 5 Pages 757-763
    Published: September 25, 1974
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    Immunologically active galactomannans isolated from the mycelia and culture supernatants of 3 representative species of the genus Hormodendrum, H. pedrosoi IFO 6071, H. compactum IFO 6726, and H. dermatitidis IFO 6421, were examined for immunological cross-reactivity with the corresponding anti-whole cell rabbit sera. The following findings were obtained.
    1) In both agar-gel double diffusion precipitin reaction and quantitative precipitin reaction, 6 galactomannans obtained from the three species of Hormodendrum showed strong cross-reactivity with rabbit antisera against whole cells of these species. The antigenic specificity of galactomannans from H. pedrosoi appeared to be closely related to or almost identical with that from H. compactum, while galactomannans from H. dermatitidis revealed antigenic specificity less closely related to galactomannans from any other species.
    The same results were obtained by the two reactions using absorbed antisera.
    2) These polysaccharides were examined for specificity by passive cutaneous anaphylaxis in guinea pigs passively sensitized with unabsorbed or absorbed antisera. Reaction patterns demonstrated by the in vivo assay were consistent with those by the in vitro assay. It was suggested that these galactomannans might be major allergens of immediate type in chromoblastomycosis.
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