Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 36, Issue 3
Displaying 1-6 of 6 articles from this issue
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  • A new K antigen of Vibrio parahaemolyticus from a strain isorated from a oversea traveller
    Shigeyuki HAMADA
    1981 Volume 36 Issue 3 Pages 557-564
    Published: May 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.557
    JOURNAL FREE ACCESS
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  • Chemical and biological properties of endotoxin of Pseudomonas aeruginosa
    Chiyoji ABE, Ken-ichi TANAMOTO
    1981 Volume 36 Issue 3 Pages 565-571
    Published: May 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.565
    JOURNAL FREE ACCESS
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  • Production in vivo and in vitro of Interferon in Mice Previously Infected with an Attenuated Strain of Salmonella enteritidis
    1. Cell Interaction in Interferon Production and Characteristics of Effective Products
    Takeshi SASAHARA, Takeshi NAKANO
    1981 Volume 36 Issue 3 Pages 573-582
    Published: May 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.573
    JOURNAL FREE ACCESS
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    The cellular source of type II interferon was studied by the use of in vitro culture systems. Splenic cells and peritoneal exudate cells were collected from mice infected with Salmonella enteritidis strain SER 2 weeks before. They were incubated with SER antigens. Interferon titres were measured in the supernatants of the cultures by using L-929 cells vs. VSV system.
    No interferon production was observed at all in the culture supernatants of splenic cells from infected athymic BALB/c mice, or in T cell-depleted splenic cell cultures from infected euthymic mice. These results suggest that T cells may have been involved in the interferon production. Purified T cells from infected euthymic mice, however, did not produce interferon. In them, the interferon production was restored by addition of splenic macrophages from noninfected athymic mice.
    When 2×106 T cell were mixed with macrophages the number of which varied from 2×106 to 3×105 or more, appreciable titres of interferon were produced by them mixed with 3×105 or more macrophages. On the other hand, when 2×106 macrophages were mixed with T cells the number of which varied from 2×106 to 3×105, the interferon production declined in proportion to the decrease in number of T cells.
    T cells were stimulated to produce interferon by antigen-pulsed splenic macrophages from non-infected athymic mice. These results strongly suggest that interferon may be produced by T cells themselves, and that macrophages may play a role as antigen-presenting cells.
    Genetic analysis of T cells and macrophages interacting in the interferon production showed that T cells should share H-2 haplotype with macrophages for the interferon production. Its result indicates that the interferon production depends upon the identity of the major histocompatibility complex between the T cells and macrophages.
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  • Mechanism of Chloramphenicol Resistance in Serratia marcescens Recently Isolated from a Clinical Material
    Michinori TERAO, Hideo MORISHITA, Sadao MIYAMURA
    1981 Volume 36 Issue 3 Pages 583-589
    Published: May 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.583
    JOURNAL FREE ACCESS
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    Chloramphenicol acetyltransferase was derived from a clinical isolate of chloramphenicolresistant Serratia marcescens. The resistance and enzyme-producing activity were transferable to Escherichia coli K-12 by conjugation. The chloramphenicol resistance appeared to be due to the presence of chloramphenicol acetyltransferase mediated by a plasmid. The subunit of the enzyme had a molecular weight of 24, 000-25, 000 and an optimum pH of 8.0. Rapid inactivation was observed at 70C. The Km for chloramphenicol was 14.3μM. The enzyme was sensitive to inhibition by 5, 5'-dithiobis-2-nitrobenzoic acid. The products of chloramphenicol obtained by inactivation were identified as 1-acetoxy, 3-acetoxy and 1, 3-diaoetoxy derivatives, respectively, by gas chromatography and thin-layer chromatography. The process of chloramphenicol acetylation was similar to that found in other organisms of Enterobacteriaceae and streptococci.
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  • A new K antigen of Vibrio parahaemolyticus from a strain isorated from a oversea traveller
    Masanori ISHIBASHI, Yoshio KINOSHITA, Yoshiaki YANAI, Hisao ABE, Yoshi ...
    1981 Volume 36 Issue 3 Pages 591-592
    Published: May 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.591
    JOURNAL FREE ACCESS
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  • New K Serovars of Vibrio parahaemolyticus
    Takeshi ITOH, Masaaki TSUNO, Kenji OHTA, Tsutomu MARUYAMA, Paradee DUM ...
    1981 Volume 36 Issue 3 Pages 593-594
    Published: May 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.593
    JOURNAL FREE ACCESS
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