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Volume 30 (1975)
- Issue 6 Pages 661-
- Issue 5 Pages 615-
- Issue 4 Pages 571-
- Issue 3 Pages 439-
- Issue 2 Pages 297-
- Issue 1 Pages 29-
Volume 30, Issue 3
Displaying 1-12 of 12 articles from this issue
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The Basic Background and ApplicationsMakoto NIWA1975 Volume 30 Issue 3 Pages 439-449
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
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1975 Volume 30 Issue 3 Pages 451-476
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
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Fusao KONDO, Manabu OGATA1975 Volume 30 Issue 3 Pages 477-485
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSClostridium perfringens strains were isolated from the intestinal contents of healthy and diarrheal animals. Of 346 fecal samples tested, 276 (79.7%) were positive for C. perfringens. The number of isolated C. perfringens organisms ranged from 102 to 108 per gram of faces. All the 276 isolates, except three, exhibited stormy fermentation by 48 hours of culture. Most of them produced α-toxin ranging from 0.1 to 0.5 α-antitoxin equivalents (AE), and only a few α-toxin of more than 2AE/ml. No significant difference could be found in α-toxigenicity between the isolates from healthy animals and those from diarrheal ones. Four isolates from guinea pigs and 11 isolates from cattle with diarrhea exhibited L-agglutinability by type D antiserum. The former 4 strains were found to be ε-toxigenic, but no ε-toxin could be demonstrated in the cultures of the latter 11 strains. Brief discussion was made on atoxic strains of C. perfringens type D.View full abstractDownload PDF (1163K) -
Michio CHO, Michinori TERAO, Hiroshi HOMMA, Kenji MIYAKOSHI, Hiroshi M ...1975 Volume 30 Issue 3 Pages 487-493
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe properties of Bordetella pertussis strains recently isolated from infants in Niigata Prefecture were studied. The K antigen type of these strains was identified as 1, 3, (6). All the strains tested showed characteristics in cultural properties and biological activities similar to those of phase I, such as heat-labile toxin, mouse body weight-decreasing toxicity, leucocytosispromoting factor, and histamine-sensitizing factor. In the mouse protection test against strain 18-323, the potency of vaccines prepared from the isolated strains was equivalent to that of Tohama phase I vaccine.
The sensitivity of the isolated strains to different antibiotics, as determined by the minimum inhibitory concentration in the serial dilution method, was as follows: penicillin G, 1U/ml; aminobenzyl penicillin, 1.25μg/ml; cephaloridine, 12.5μg/ml; cephazolin, 12.5μg/ml; polymyxin B, 2U/ml; colistin, 2U/ml; streptomycin, 10μg/ml; kanamycin, 12.5μg/ml; tetracycline, 0.25μg/ml; chloramphenicol, 0.5μg/ml; and erythromycin, 0.05μg/ml.View full abstractDownload PDF (854K) -
Isamu TANI, Tomoko SAGARA, Hirofumi SHIBATA1975 Volume 30 Issue 3 Pages 495-499
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSWhen formed in modified G medium, spores of Bacillus cereus T were heat-resistant. They were heat-sensitive, however, as compared with normal spores, when formed in modified G medium supplemented with 6mg/ml of glycine (glycine spores).
Some properties in germination were studied in normal and glycine spores which were different from each other in heat-resistance. When effects of temperature and pH on L-alanineinduced germination were tested, both spores showed similar results: an optimal temperature and an optimal pH were 30°C and 8.0, respectively. When these spores were examined for requirement of any L-amino acid as a germinant and for inhibition by D-alanine of L-alanine induced germination, the same results were also obtained from them, except the following two points.
(1) Nine L-amino acids, L-alanine, L-cysteine, L-glutamine, L-isoleucine, L-leucine, L-methionine, L-serine, L-threonine and L-valine, were effective as germinants for normal spores. Four of them, L-isoleucine, L-leucine, L-threonine and L-valine, were not so effective as the other five for glycine spores.
(2) Inhibition by D-alanine of L-alanine-induced germination was much stronger to glycine spores than normal spores.
These results suggest that sporulation in the presence of a high concentration of glycine may have resulted in the conformational changes of the recognition sites on germinants, as well as in the decreased heat resistance of spores of B. cereus T.View full abstractDownload PDF (581K) -
Mitsuru WATANABE, Kachiko SEKIYA, Yasukiyo NAKASE, Mitsuo YAMADA1975 Volume 30 Issue 3 Pages 501-505
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDrug sensitivity of 67 strains of Bordetella pertussis and 10 strains of B. parapertussis isolated from patients in an outbreak of whooping cough in Miura Peninsula over a period from 1972 to 1973 was determined. It was compared with that of 25 strains of B. pertussis and 9 strains of B. parapertussis isolated over a period from 1952 to 1953 and preserved by lyophilization of subculture. The results obtained are summarized as follows.
(1) The mean minimal inhibitory concentrations (MIC) against the 92 strains of B. pertussis ranged from ≤0.1 to 0.8units/ml for penicillin (PC), from ≤0.1 to 0.4mcg/ml for aminobenzylpenicillin (AB-PC), from 0.8 to 12.5mcg/ml for methyiphenylisoxazolylpenicillin (MPI-PC), from 12.5 to 100mcg/ml for cephalexin (CEX), from ≤0.1 to 3.1mcg/ml for sulfamonomethoxine (SA), from 0.2 to 3.1mcg/ml for streptomysin (SM), from ≤0.1 to 0.8mcg/ml for chioramphenicol (CP), from ≤0.1 to 0.2mcg/ml for tetracycline (TC), and from ≤0.1 to 3.1mcg/ml for nalidixic acid (NA).
(2) The range MIC against the 19 strains of B. parapertussis were in the following: 6.3 to 50units/ml for PC, 0.8 to 3.1mcg/ml for AB-PC, ≥300mcg/ml for MPI-PC, 100 to 200mcg/ml for CEX, ≤0.1 to 1.6mcg/ml for SA, 25 to 100mcg/ml for SM, 0.4 to 0.8mcg/ml for CP, ≤0.1 to 0.4mcg/ml for TC, and 0.8 to 3.1mcg/ml for NA.
(3) CEX, SA, CP, TC, and NA showed almost the same degrees of MIC against the 19 strains of B. parapertussis as against B. pertussis. PC, AB-PC, MPI-PC, and SM showed apparently different degrees of MIC against B. parapertussis from those against B. pertussis.
(4) No drug-resistant strains were detected from the strains of B. pertussis or B. parapertussis isolated.
(5) There was no difference in drug sensitivity between the recently isolated strains and the strains isolated about 20 years ago, regardless of the species.View full abstractDownload PDF (610K) -
Kachiko SEKIYA, Mitsuru WATANABE, Yasukiyo NAKASE, Mitsuo YAMADA1975 Volume 30 Issue 3 Pages 507-513
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSFrom 832 cough plates, 71 strains of Bordetella pertussis and 8 strains of B. parapertussis were isolated and identified during a period from June, 1972 to February, 1974, in an outbreak of whooping cough in the Miura Peninsula district.
Serotyping of the B. pertussis strains was carried out by the slide agglutination test using monospecific factor sera. Ten strains (14.1%) belonged to serotype 1-2-3-4, 12 strains (16.9%) to 1-3, and 49 strains (69.0%) to 1-3-4, including 2 strains which showed a faint reaction to serotype 3 and 4, respectively.
No significant differences in serotype were observed among strains isolated from patients unvaccinated (44 strains), incompletely vaccinated (17 strains), and completely vaccinated (8 strains). No strains having factor 2 were found among those isolated from patients more than 8 years of age. Relationship between serotype and phase variation in B. pertussis was discussed from the results obtained.View full abstractDownload PDF (724K) -
Yuuji TERADA, Yutaka YOKOO, Hisao NAKANISHI, Tadashi TERAMOTO1975 Volume 30 Issue 3 Pages 515-516
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
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1975 Volume 30 Issue 3 Pages 517-531
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
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1975 Volume 30 Issue 3 Pages 533-548
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
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1975 Volume 30 Issue 3 Pages 549-555
Published: May 25, 1975
Released on J-STAGE: February 19, 2009
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1975 Volume 30 Issue 3 Pages 568
Published: 1975
Released on J-STAGE: February 19, 2009
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