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Volume 32 (1977)
- Issue 6 Pages 777-
- Issue 5 Pages 655-
- Issue 4 Pages 533-
- Issue 3 Pages 455-
- Issue 2 Pages 337-
- Issue 1 Pages 31-
Volume 32, Issue 2
Displaying 1-9 of 9 articles from this issue
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Yoshiki OBANA, Shozo NAKAZAWA1977 Volume 32 Issue 2 Pages 337-343
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSWhen treated with sulbenicillin, Pseudomonas aeruginosa showed suppressed growth and its cells were elongated into filamentous profiles. These cells were examined for morphology, biochemical properties, and pathogenicity.
1) The bacterial cells underwent no prominent morphological changes, except that they became remarkably elongated.
2) The filamentous cells were less resistant to heat and ultrasonic waves and more sensitive to phenol, lysozyme, and gentamicin than the intact cells.
3) The filamentous cells were actively ingested and destroyed by phagocytes, but they intact cells were not.
4) The filamentous cells were much less pathogenic for mice than the intact cells.
5) Endotoxin of the filamentous cells produced no significant toxic effect, while endotoxin of the intact cells was markedly toxic.
6) The filamentous cells were different from the intact cells in certain biochemical properties and significantly lower in pathogenicity and toxicity than the intact ones. These findings indicate that when used for chemotherapy, sulbenicillin not only exerts a direct bactericidal effect but inhibits bacterial growth and produces additional therapeutic effects.View full abstractDownload PDF (3059K) -
Sumio SHINODA, Noriko NAKAHARA, Yoshifumi TAKEDA, Toshio MIWATANI1977 Volume 32 Issue 2 Pages 345-351
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSSome detergents were studied for effect on the flagellation of bacteria. When bacteria were grown on agar medium containing anionic detergents, such as sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, and Teepol, lateral or peritrichous flagella could not be found, but a polar monotrichous flagellum was apparent. Nonionic detergents, such as Triton X-100 and Tween 80, and a cationic detergent, benzalkonium chloride, did not affect the flagellation so long as they were at a concentration which allowed normal growth of bacteria.
The absence of lateral flagella of Vibrio parahaemolyticus grown on solid agar medium containing detergents was not due to the solubilization of flagella by detergents after the flagellar formation, but to the inhibition of flagellar protein synthesis per se.
V. parahaemolyticus possessed lateral and polar monotrichous flagella and was motile in liquid or soft agar medium containing anionic detergents. It could not swarm solid agar medium containing anionic detergents, since it lost its lateral flagella due to the presence of detergents.View full abstractDownload PDF (2639K) -
Masaharu KITASATO, Nobutaka OSAWA, Noboru YAMAURA, Yoshihiro USHIYAMA, ...1977 Volume 32 Issue 2 Pages 353-361
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThis work concerns the involvement of brown adipose tissue in the immune system of the mouse. To remove the paired lobes of the interscapular brown adipose tissue, neonatal adipectomy was performed in dd, C3H/He, C57BL/6, and BALB/C mice of both sexes. These mice were examined at 6-8 weeks of age for the production of antibodies against sheep red blood cells (SRBC) and flagellin of Salmonella adelaide, delayed-type skin reaction to picryl chloride, quantitative estimation of the macrophage migration inhibition to ovalbumin injected with Freund's complete adjuvant, and acquisition of resistance to reinfection after inoculation with attenuated live S. enteritidis vaccine. Neonatal adipectomy enhanced the production of anti-SRBC (by the direct method alone) and anti-flagellin antibodies. There was a discrepancy between these findings and those of Jankovic et al. who reported that neonatal adipectomy did not affect the humoral immune response of rats to bovine serum albumin or SRBC. Cell-mediated immunity to picryl chloride, ovalbumin, and salmonella infection was much more pronounced in adipectomized mice than in intact mice. These results were in complete agreement with those obtained by Jankovic et al. from rats.
This paper also describes postnatal histological changes of the interscapular brown adipose tissue of mice, including newborn and 6-month-old ones, which were reared at room temperature without receiving any particular treatment.View full abstractDownload PDF (3657K) -
Yukinori TSUNEMATSU, Kiseko KAMEI, Keisuke MIZUMA, Yoshio KATANIWA, Yo ...1977 Volume 32 Issue 2 Pages 363-368
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSLincomycin (2μg/ml)-colistin (20U/ml) medium (LC medium) devised by Shiozawa and Tsunematsu (1967) was investigated for usefulness in comparison with vancomycin-colistimethatenystatin medium (VCN medium) developed by Thayer and Martin (1966). Cervical and rectal specimens from 97 street prostitutes in Tokyo were used in the present study with the following results.
(1) The total number of infected persons was 15 (15.5%). Cervical specimens taken from 13 persons yielded gonococci on both media, and those taken from 2 persons gave positive results on LC medium only. Three rectal specimens yielded gonococci on LC medium, but one of them failed to do so on VCN medium. Rectal infection was found only among persons with cervical infection. (2) The growth on LC medium of contaminant bacteria, especially of Gram-positive ones, was comparable with that on VCN medium. (3) MIC of lincomycin was 8μg/ml against the 2 most susceptible strains of 26 gonococcal strains originated from the prostitutes and male patients. MICs for vancomycin ranged from 1.0 to 64μg/ml. Three of 22 strains showed MICs below 3μg/ml.
On the basis of the findings mentioned above, lincomycin was assumed to be a preferable substitute for vancomycin, should it be used at a lower concentration (2μg/ml or so) than those adopted by other workers (5-10μg/ml).View full abstractDownload PDF (866K) -
Tsutomu SHIMONO1977 Volume 32 Issue 2 Pages 369-391
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSBCG cells were grown homogeneously in a 1% Tween 80-casamino acid medium by shaking. They were submitted to gentle fractionation procedures by use of a Ribi refrigerated cell fractionator equipped with the French pressure cell and detergents (Triton X-100 and SDS) in order to isolate the cell surface components, cell walls, cell membranes, and cytoplasm. Each of the subcellular structures and components isolated in this manner was found to exhibit characteristic immunological and chemical properties.
The cell-wall fraction obtained by extraction of the cell envelope with detergents was shown to contain an antigen protein the presence, role, and significance of which had been overlooked in the cell walls purified by protease digestion.View full abstractDownload PDF (20779K) -
Mituo TOKORO, Kiichi GOTO, Fuzizo YAMADA1977 Volume 32 Issue 2 Pages 393-394
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (203K) -
1977 Volume 32 Issue 2 Pages 395-402
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1250K) -
1977 Volume 32 Issue 2 Pages 403-419
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2789K) -
1977 Volume 32 Issue 2 Pages 421-430
Published: March 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1593K)
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