Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 36, Issue 2
Displaying 1-11 of 11 articles from this issue
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  • Structure and action of staphylococcal alpha-toxin and leukocidin
    Iwao KATO
    1981 Volume 36 Issue 2 Pages 445-457
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.445
    JOURNAL FREE ACCESS
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  • Incidence and survival of Clostridium perfringens in soil
    Kenichi KONISHI, Takayoshi YAMAGISHI, Shintaro ISHISAKA, Kenichi SAKAM ...
    1981 Volume 36 Issue 2 Pages 459-464
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.459
    JOURNAL FREE ACCESS
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    The incidence of Clostridium perfringens was 100% in soil specimens collected from formerly developed areas, such as cultivated field, public park and playground. On the contrary, it was 10 to 90% in those collected from newly developed areas, such as soccer ground, baseball ground, tennis court, turf and the site of construction. A high incidence of the organism was observed in the soil of areas well cultivated, frequently utilized or trodden by people. The number of C. perfringens cells found in one gram of soil ranged from 7 to 900. The isolates from soil specimens were examined for survival in a sterilized 50% soil suspension. Some of them rapidly formed thermostabile cells resistant to heating at 75C for 15min. The others were not heat-resistant. Both types of the isolates survived for more than 4 weeks, although the heat-resistant cells survived relatively long with a slight decrease in number. The isolates from soil were examined for survival in a 50% soil suspension, α-toxigenicity and salt tolerance, in comparison with those from human feces, but no differences were observed between both groups of isolates. The finding of the present study suggests that no multiplication of C. perfringens may occur in soil.
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  • Transferable R Plasmids Isolated from Vibrio cholerae
    Takako KANOH
    1981 Volume 36 Issue 2 Pages 465-474
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.465
    JOURNAL FREE ACCESS
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    This report deals with the genetic and molecular characteristics of four transferable R plasmids isolated from Vibrio cholerae (biotype El Tor) which was isolated in Nigeria and East Asia in 1977 and 1978, respectively.
    1) The R plasmids conferred a resistance to all or some of chloramphenicol (CP), tetracycline (TC), streptomycin (SM), sulfonamide (SA), kanamycin (KM) and ampicillin (ABPC) on the host bacteria. The levels of resistance conferred by them were high to SM, KM, SA, and ABPC, moderate to CM, and markdely low to TC.
    2) All the R plasmids tested were conjugative. Their frequencies of transfer per donor by 2-hour conjugation were approximately from 10-4 to 10-5 for pJT-17 pJT-45, and from 10-2 to 10-3 for pJT-12 and pJT-13.
    3) The plasmids were non-thermosensitive in both transferability and replication.
    4) The plasmids belonged to the incompatibility C group.
    5) The plasmids were stable in both Vibrio cholerae and Escherichia coli even after ten subcultures in drug-free media.
    6) The molecular weights of CCC DNA plasmids, pJT-17 and pJT-45, were approximately 90 and 99 megadaltons, respectively, and those of pJT-12 and pJT-13 were both 106 megadaltons.
    7) Two R plasmids, pJT-17 and pJT-45, showed a temporary but dramatic increase (10, 000-fold) in transfer frequency during the logarithmic phase, as compared with the stationary phase. This phenomenon during the logarithmic phase was accompanied with the formation of multiple sex pili on the surface of Escherichia coli negative for general pili.
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  • A New K Serovar of Vibrio parahaemolyticus
    Masaaki TSUNO, Kenji OHTA, Shigeru MATSUSHITA, Senzo SAKAI
    1981 Volume 36 Issue 2 Pages 475-476
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.475
    JOURNAL FREE ACCESS
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  • Erratum to : A medium for the rapid glucose fermentation test to distinguish coagulase-negative staphylococci from micrococci
    1981 Volume 36 Issue 2 Pages 477
    Published: 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.2_477
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1981 Volume 36 Issue 2 Pages 479-489
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.479
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1981 Volume 36 Issue 2 Pages 490-498
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.490
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1981 Volume 36 Issue 2 Pages 499-516
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.499
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1981 Volume 36 Issue 2 Pages 517-528
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.517
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1981 Volume 36 Issue 2 Pages 529-543
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.529
    JOURNAL FREE ACCESS
    Download PDF (2547K)
  • [title in Japanese]
    1981 Volume 36 Issue 2 Pages 544-546
    Published: March 25, 1981
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.36.544
    JOURNAL FREE ACCESS
    Download PDF (437K)
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