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Volume 32 (1977)
- Issue 6 Pages 777-
- Issue 5 Pages 655-
- Issue 4 Pages 533-
- Issue 3 Pages 455-
- Issue 2 Pages 337-
- Issue 1 Pages 31-
Volume 32, Issue 4
Displaying 1-8 of 8 articles from this issue
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Part. 2 Electron microscopic studies, with special reference to the static morphologyZensaku YOSHII1977 Volume 32 Issue 4 Pages 533-558
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
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Toshio MIWATANI, Eiko YABUUCHI1977 Volume 32 Issue 4 Pages 559-569
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
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Junpei KOIKE1977 Volume 32 Issue 4 Pages 571-577
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe most adequate conditions for DNA-DNA hybridization were studied by using DNA isolated from Staphylococcus aureus.
1) Nonspecific adsorption of sheared denatured DNA (SD-DNA) to a membrane filter decreased remarkably by preincubation in a mixture of 1/2×PM (PM containing 0.02% each of ficoll, polyvinyl pyrrolidone and bovine serum albumin) and 6×SSC (SSC containing 0.15M NaCl and 0.015M trisodium citrate, at pH 7.0) at 60C for 6 hours.
2) A release of immobilized DNA (IM-DNA) on a membrane filter from the filter occurred only 15% during the hybridization reaction of 0.003M MgCl2 and 0.1% sodium dodecyl sulfate in 6×SSC at 60C for 20 hours.
3) The concentration of IM-DNA required was more than 6μg/filter against 0.1-0.5μg of SD-DNA.
4) The chain length of SD-DNA affected the ratio of DNA reassociation. About 300 nucleotide units showed the maximum ratio of DNA reassociation.
5) Incubation temperature gave the maximum ratio of DNA reassociation at 60C.
6) An incubation period of 20 hours gave rise to a maximum reassociation value.
The melting temperature (Tm) of reassociated DNA was 76.5C in the most adequate condition, while Tm of native DNA was 77.0C.View full abstractDownload PDF (725K) -
Junpei KOIKE, Kazuko A. KOIKE, Kikuo FUJIWARA1977 Volume 32 Issue 4 Pages 579-584
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDNA-DNA hybridization experiments were carried out in order to determine the genetic homologies among Staphylococcus aureus and related organisms. The strains of Micrococcus luteus, Escherichia coli and Streptomyces aureofaciens were examined as control organisms.
When nonenterotoxigenic S. aureus 209p was used as reference DNA, DNA reassociation experiments showed genetic homology values ranging from 52 to 75% with seven enterotoxigenic strains, from 87 to 100% with two nontoxigenic S. aureus and from 25 to 43% with five strains of S. epidermidis, while M. luteus belonging to the same Micrococcaceae exhibited a very low value of homology similar to those of E. coli and Str. aureofaciens.
On the contrary, when enterotoxigenic S. aureus S-6 (enterotoxin B) was used as reference DNA, the genetic homology value ranged from 80 to 100% with enterotoxigenic strains, from 63 to 69% with nontoxigenic S. aureus, and from 44 to 66% with S. epidermidis. It was 28% with M. luteus and 24.1% with E. coli and Str. aureofaciens.
These results indicate that S. aureus, S. epidermidis and M. luteus can be distinguished from one another on the basis of DNA homology value. In addition, they suggest that the genetic relatedness may be useful for the detection of enterotoxin-producing strains of S. aureus.View full abstractDownload PDF (703K) -
Kaoru KOSHIMIZU, Teruo MAGARIBUCHI1977 Volume 32 Issue 4 Pages 585-595
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSMicrobiologic examinations have been conducted on a total of 131 non-human primates imported from Southeast Asia, Africa and South America in an attempt to detect Ureaplasma (T-Mycoplasma) on five different occasions during a period from 1973 to 1976. The animals included 113 cynomolgus monkeys (Macaca irus), 9 green monkeys (Cercopithecus aethiops) and 9 common squirrel monkeys (Saimiri sciurea). Most of them were apparently healthy at the time of examination. Ureaplasmas were detected from cynomolgus monkeys (4/20: 20%), green monkeys (5/9: 56%), and from common squirrel monkeys (6/9: 67%) examined on the first occasion in 1973 only. When 215 specimens obtained from these 38 monkeys were examined, Ureaplasmas were isolated from 20 specimens (9%), which had been collected from the nasal cavity of 4 animals (11%), the oral cavity of 10 (26%), the male urethra of0 4 (17%) and rectal feces of 2 (5%). No Ureaplasma was isolated from the vagina, liver, kidneys, spleen, lungs, hilar lymph nodes, or blood.
The ureaplasmal isolates grew on solid medium, forming characteristically tiny colonies about 38μm in diameter. Electron microscopic observation revealed large and small particles about 200 to 500nm in diameter surrounded by a characteristic triple-layered unit membrane, devoid of a cell wall. The organisms did not grow in serum-free medium and were proved to have an ability to utilize urea contained in the medium actively for 24 hours of incubation. They formed a film and spots on a solid medium. Serological studies by the metabolism-inhibition test demonstrated an antigenic similarity among all the isolates, some of which showed an antigenic relationship to Black's serotypes I, III, VI, VII and VIII of U. urealyticum.
The biological and serological properties of the simian isolates indicate that it is appropriate to classify these isolates into the category of subspecies of U. urealyticum of human origin.View full abstractDownload PDF (8957K) -
Takayuki SASAKI1977 Volume 32 Issue 4 Pages 597-602
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSRabbits thymectomized within 2 months after birth presented a more marked anti-hapten antibody response against DNP-BGG than non-thymectomized control animals. A reduction in anti-hapten response was observed in rabbits injected with allogeneic thymocytes, although it was of short duration. A marked reduction in anti-hapten response was observed in rabbits pre-immunized with the carrier, showing a sharp contrast with an ordinary response observed in the mouse.
The results mentioned above seemed to show the possibility that the immunological function of the rabbit T cells might be qualitatively or quantitatively different from that of the mouse T cells.View full abstractDownload PDF (860K) -
Hiroo IIDA, Mitsuko SHIOZAKI1977 Volume 32 Issue 4 Pages 603-604
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
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1977 Volume 32 Issue 4 Pages 605-607
Published: July 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (421K)
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