Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 27, Issue 4
Displaying 1-4 of 4 articles from this issue
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  • Recent Advances in the Research of Fungal Toxins with Special Referrence to High Molecular Weight Toxins
    Kazuo IWATA
    1972 Volume 27 Issue 4 Pages 563-576
    Published: July 25, 1972
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.27.563
    JOURNAL FREE ACCESS
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  • Studies on the Relationship between Growth and Substrate Oxidation Activity in Pseudomonas fragi and Pseudomonas aervginosa
    Yotaku GYOBU, Shigeichi YAMAZAKI, Kentaro KUBOTA
    1972 Volume 27 Issue 4 Pages 577-584
    Published: July 25, 1972
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.27.577
    JOURNAL FREE ACCESS
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    The effect of temperature on the growth and substrate oxidation activity in Pseudomonas fragi (as a psychrophile) and P. aeruginosa (as a mesophile) was investigated. The results obtained are summarized as follows.
    1. Both organisms showed essentially the same temperature-characteristic patterns of growth rate, except that the temperature-optima for growth of P. fragi and P. aeruginosa were 25 and 35C, respectively.
    2. Both organisms resembled each other with regard to the temperature-characteristic patterns of malate or succinate oxidation and those of several dehyrogenase activities within a range from 5 to 35C.
    3. In a higher temperature range, for instance, at 45 C, P. aeruginosa oxidized pyruvate, ethanol, acetate, and malate more effectively than P. fragi did, and vice versa in a lower tempetaure range, except for malate oxidation.
    44. The temperature-characteristic patterns of malate oxidation activity in intact cells were similar to those in dried cells of each Pseudomonas species.
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  • A New Serologic Diagnostic Method of Syphilis with Treponema pallidum Cell Components Adsorbed in Sensitized Carbon Particles as Antigen
    Hayashi AWANO
    1972 Volume 27 Issue 4 Pages 585-591
    Published: July 25, 1972
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.27.585
    JOURNAL FREE ACCESS
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    A new serologic method was devised for the diagnosis of syphilis. It was an indirect agglutination reaction for which sonicated virulent Trepanema pallidum (TP) cell components served as antigen after adsorbed in carbon particles 5-10 microns in diameter (prepared from charcoal by refining). This reaction was studied for conditions of occurrence.
    This antigen consisting of TP-sensitized carbon particles was mixed with human or rabbit syphilitic serum adsorbed in coarse carbon powder sensitized with TP Reiter strain cell components, or with solutions of γ and β-globulin separated from syphilitic serum. Then, a typical accumulation shape of antigen-antibody combination, discernible by the naked eye, was formed at the bottom of the test tube held at 22C for approximately 30-40 minutes.
    This antigen did not react at all to human or rabbit non-syphilitic serum when subjected to the absorption process. It did not produce an accumulation shape, but precipitated at the bottom of the test tube to form an antigen film.
    It is believed that this method is characterized by excellent specificity, because it is handy, requires only short reaction time, and dispenses with the introduction of serum or macromolecular chemical compounds of high concentration into the reacting solution.
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  • [title in Japanese]
    1972 Volume 27 Issue 4 Pages 593-602
    Published: July 25, 1972
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.27.593
    JOURNAL FREE ACCESS
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