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Volume 33 (1978)
- Issue 6 Pages 715-
- Issue 5 Pages 673-
- Issue 4 Pages 607-
- Issue 3 Pages 505-
- Issue 2 Pages 355-
- Issue 1 Pages 33-
Volume 33, Issue 2
Displaying 1-14 of 14 articles from this issue
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Katsutoshi MISE, Kenji SUZUKI, Rintaro NAKAYA1978 Volume 33 Issue 2 Pages 355-367
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 369-387
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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Kenji KOHSAKA, Masanao MAKINO, Tatsuo MORI, Tonetaro ITO1978 Volume 33 Issue 2 Pages 389-394
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA lepromatoid lesion developed in a nude mouse inoculated with Mycobacterium leprae was previously reported by the authors. The secondary passage of M. leprae which had proliferated in the lesion of the first infected nude mouse into other nude mice was confirmed experimentally. The reproducibility of animal transmission with nude mice was also proved.
Successive transmission of M. leprae was carried out three times by the foot pad technique with the organism which had proliferated in a nude mouse. M. leprae derived from 5 lepromatous patients was also inoculated into foot pads of nude mice. Infected animals were maintained in vinyl (plastic) isolators under an SPF condition.
Swelling was found macroscopically in infected foot pads of all the animals in the 10th month after infection. A lepromatoid lesion was seen at the site of inoculation. At the same time, a bacterial harvest amounted to 3.6×108 from a foot pad of the mouse. The nude mouse (BALB/c-nu/nu) and its normal littermate (BALB/c-nu/+) were examined for body temperature with an electronic thermometer. There was no significant difference in body temperature between the nude mouse and the normal. M. leprae was detected from the skin of low-temperatured parts of the body, but not from the skin of high-temperatured parts, in the 10th month after infection. It was seen in lung, liver and spleen, but not in the kidney. M. leprae organisms derived from 5 different patients were successfully transmitted into the foot pads of nude mice. The maximum yield of M. leprae was 1.1×1010 in a foot pad in the 8th month after infection.View full abstractDownload PDF (2377K) -
Yoshiko HIRATA1978 Volume 33 Issue 2 Pages 395-402
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe growth of Streptococcus mutans was inhibited by 1.5% sodium citrate added to some basal media. Such inhibition was also recognized in trypticase soy broth medium containing 5% sucrose and 1.5% citrate. Sodium citrate had no inhibitory effects on the growth of any other streptococcal type strain (S. sanguis, S. salivarius, S. mitis, S. MG, Group A S. pyogenes, or Group K streptococci) or Staphylococcus aureus.
When added to trypticase soy broth medium where S. mutans had been incubated, sodium citrate showed an inhibitory effect on the streptococcal growth in the early stage. No such inhibition was observed in a culture medium which contained sodium citrate and to which divalent cations, such as Mn++, Ca++, and Mg++, had been added. EDTA, a potent chelating agent, was also able to inhibit the growth of S. mutans. Sodium citrate did not inhibit glucose utilization by S. mutans.
Sodium citrate inhibited the growth of 96-100% of the strains which had been isolated from the dental plaques of healthy individuals and from carious lesions and which had fermented both mannitol and sorbitol. It did not inhibit the growth of 70% of the strains which failed to ferment mannitol and sorbitol.
From these results, it seems that sodium citrate may have inhibited the growth of S. mutans, since it has a chelating activity on divalent cations which are essential for the growth of S. mutans. In addition, these findings suggest that the failure of growing in the presence of sodium citrate may be one of the biological and biochemical properties of S. mutans useful for the identification of this organism.View full abstractDownload PDF (898K) -
Izumi MOCHIZUKI1978 Volume 33 Issue 2 Pages 403-413
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThis study made it clear that for the initial isolation and subculture of Bacteroides melaninogenicus CO2 gas at a concentration of 50% or more was needed in addition to the standard conditions. With regard to black colony formation, laked rabbit blood agar was superior to an agar preparation containing blood of any other animal (sheep, horse, man, or chicken). When ovine, equine or human blood was used, many strains, even those of B. melaninogenicus, failed to form black colonies.
To isolate B. melaninogenicus from various specimens, Brucella agar (BBL) with 5% laked rabbit blood cultured by the steelwool method (CO2 50%, N2 50%) for 1 week was used. The isolated strains were subcultured in PRAS-semisolid GAM medium (Nissui) and identified according to the VPI Manual and Bergey's Manual (8th edition).
B. melaninogenicus ss. asaccharolyticus was isolated from vaginal swabs, B. melaninogenicus ss. intermedius from the gingival sulcus (58.3%), vaginal swabs (33.3%), and feces (8.4%), and B. melaninogenicus ss. melaninogenicus from the gingival sulcus alone.
Biochemicral characteristics of all the isolates were determined. A scheme was developed for the subspecific classification of B. melaninogenicus.View full abstractDownload PDF (1226K) -
Shoichiro OHARA, Tadashi SATO, Morio HOMMA1978 Volume 33 Issue 2 Pages 415-420
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA total of 112 strains of Francisella tularensis isolated from different countries and from different sources were tested for susceptibility to various antibiotics. All of them were uniformly not susceptible to penicillin, cephalosporin C and polypeptide antibiotics, but were all susceptible to aminoglycoside, chloramphenicol and tetracycline. As to macrolide antibiotics, however, they belonged to either highly sensitive or highly resistant group. The former group consisted of strains isolated from China, Italy, Japan, and the U.S.A., and the latter one of strains from the U.S.S.R. and East European countries. These results are quite consistent with those reported by Russian workers, who observed that the susceptibility of the organism to erythromycin differed according to the place from which the organism had been isolated.
The results mentioned above seem to show that the susceptibility of F. tularensis to macrolide antibiotics may be a genetic marker which is independent of the virulence and other biochemical properties of the organism and may offer a basis for understanding the origins of various strains of the organism.View full abstractDownload PDF (630K) -
Hidenobu OZAKI, Yuuji TERADA1978 Volume 33 Issue 2 Pages 421-422
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 423-429
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 431-438
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 439-448
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 449-460
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 461-471
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 472-484
Published: March 25, 1978
Released on J-STAGE: February 19, 2009
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1978 Volume 33 Issue 2 Pages 501
Published: 1978
Released on J-STAGE: February 19, 2009
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