Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 33, Issue 3
Displaying 1-6 of 6 articles from this issue
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  • Cytology of Spirochetal Cell
    Part. 3 Electron Microscopic Studies, with Special Reference to the Dynamic Morphology
    Zensaku YOSHII
    1978 Volume 33 Issue 3 Pages 505-526
    Published: May 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.505
    JOURNAL FREE ACCESS
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  • Comparison of Cell Body and Cell Wall Compositions between the Yeast-like and Mycelial Phases of Geotrichum candidum
    Keiichi EBINA, Shinobu TAKASHITA, Arihide KAMAGUCHI, Katsushi YOKOTA, ...
    1978 Volume 33 Issue 3 Pages 527-538
    Published: May 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.527
    JOURNAL FREE ACCESS
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    Cells of yeast-like (Y) and mycelial (M) phases were obtained from cultures of Geotrichum candidum on basal media containing 1% glucose and 0.5% glycerol, respectively. Their cell walls were fractionated by successive treatment with sonic vibration and centrifugation.
    The Y-phase cells contained slightly larger amounts of protein, RNA and phosphorus (12.0%, 1.9%, and 0.8%) than the M-phase cells (9.0%, 1.2%, and 0.6%), whereas the hexosamine content of the M-cells (9.7%) was slightly greater than that of the Y-cells (8.0%).
    There was no significant difference between the two phases in the amount of total sugar (Y: 57%, M: 60%), acid-or alkali-soluble polysaccharides, or lipids (Y: 6%, M: 5%). The amounts of galactose and mannose in the supernatant obtained after sonic vibration were found larger in the M-phase cells than in the Y-phase cells. The ratio of the amount of galactose plus mannose in the supernatant to that of glucose in the cell wall was approximately five percent of the molar ratio.
    The wall lipid of the M-cells extracted with CHCl3-MeOH contained fatty acids, such as C14, C15, C16, C16:1, C17, C17:1, C18, C18:1, C18:2 and C19:1, at a molar ratio of 1:2:17:2:2:3:4:38:30:1. Therefore, the cell wall contained three times as much unsaturated fatty acids as saturated fatty acids.
    Amino acid analyses of the wall protein of cells of both phases revealed that the Y-cells had a little more aspartic acid, glutamic acid and glycine (9.0%, 9.7%, and 8.1%) than the M-cells (8.2%, 8.5%, and 5.9%), and that the M-cells had a little more alanine (12.8%) than the Y-cells (9.8%).
    These results suggest that there may be no qualitative difference in the chemical composition of the cell wall between the cells of both phases, although small differences may be seen in the contents of several components and/or the composition between these cells.
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  • Salt Tolerance Mechanism of Staphylococcus aureus
    Energy Metabolism at High Salinity
    Hideo HAYASHI, Izumi KOUJIMA, Ken-ichiro KIGUCHI, Yasuhiro KANEMASA
    1978 Volume 33 Issue 3 Pages 539-546
    Published: May 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.539
    JOURNAL FREE ACCESS
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    The salt tolerance mechanism of Staphylococcus aureus was examined from a view point of energy metabolism in the cell membrane. Growth and oxygen consumption were reduced in rate and pyruvate kinase activity was enchanced in S. aureus in the presence of 1.8M NaCl in medium. The membrane fraction isolated from the cells grown in medium containing 1.8M NaCl, however, showed almost the same activity of NADH-, succinic-, lactate-oxidase, Mg-ATPase and ouabain-insensitive Na/K-ATPase as that from the cells grown in medium at normal salinity. There was little difference in the effects of inhibitors (Na2 HAsO4, KCN, and 2, 4-DNP) on the membrane between the cells grown in the two media of different salinity, Cytochrome contents and SDS-polyacrylamide gel electrophoresis patterns were also alike. These results show that energy metabolism in the staphylococcal cell membrane was accomplished in the same manner at high salinity as at normal salinity.
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  • Note on the Staining Effects of Dyes for Treponema pallidum (Pathogenic Nichols Strain)
    Yoshihiro SAIJO, Ichiro HIRATA, Hiroko ONODA
    1978 Volume 33 Issue 3 Pages 547-550
    Published: May 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.547
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1978 Volume 33 Issue 3 Pages 551-564
    Published: May 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.551
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1978 Volume 33 Issue 3 Pages 565-578
    Published: May 25, 1978
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.33.565
    JOURNAL FREE ACCESS
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