All issues
Volume 35 (1980)
- Issue 6 Pages 721-
- Issue 5 Pages 673-
- Issue 4 Pages 581-
- Issue 3 Pages 517-
- Issue 2 Pages 405-
- Issue 1 Pages 36-
Volume 35, Issue 4
Displaying 1-5 of 5 articles from this issue
- |<
- <
- 1
- >
- >|
-
1980 Volume 35 Issue 4 Pages 581-603
Published: July 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (4101K) -
Differences in lesions caused between Staphylococcus aureus 308A-1 and the unique staphylococcal strain, E-97Toshiyuki YAMAZAKI1980 Volume 35 Issue 4 Pages 605-624
Published: July 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA lesion caused by experimental intraperitoneal infection in mice was compared betaween two staphylococcal strains. Staphylococci contaminating the surface of the abdominal organs were efficiently eliminated by soaking the organs into 3% cresol for 20 minutes. This treatment did not significantly affect the viability of bacteria in the interior of any organ.
When Staphylococcus aureus 308A-1 suspended in 5% mucin was injected into mice, it proliferated mainly in the peritoneal cavity and grew on the surface of the abdominal organs, where characteristic greyish white products were concurrently observed. Bacteriological and morphological studies revealed that the products had resulted from the colonization of 308A-1. In the blood, a few staphylococci were demonstrated in the early stage of infection and thereafter.
The unique staphylococcal strain, E-97 did not produce coagulase but formed diffusetype colonies on plasma-soft agar. In mice infected with it, no such products were observed on the surface of any organ. Strain E-97 proliferated mainly in the peritoneal cavity, as well as 308A-1. It penetrated, however, into the blood to a much larger extent than 308A-1. Furthermore, a large number of staphylococci was found in the reticuloendothelial cells of the liver and the spleen in the early stage of infection and thereafter, and a few of them in the interior of the organs of mice infected with 308A-1.View full abstractDownload PDF (9002K) -
Tsutomu USHIJIMA, Yoshikatsu OZAKI1980 Volume 35 Issue 4 Pages 625-637
Published: July 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDetailed biochemical tests were performed for the characterization of 5 strains labeled of Bacillus subtilis, 3 strains of Bacillus natto and 10 strains isolated recently from natto in the market.
1) All the strains tested were identified as Bacillus subtilis on the basis of Bergey's Manual (8th edition).
2) On the basis of 13 characteristics, all the strains were divided into 2 groups, A and B. Group A consisted of 4 strains labeled of B. subtilis (ATCC6051 neotype strains, ATCC6633, and NIHJ-PCI219) and B. natto (SC530123). Group B was composed of 2 strains labeled of B. subtilis (IFO3009 and IFO13169), and 12 strains isolated from natto.
3) Key characters for differentiation between the two groups were acid production from glycogen, complete hydrolysis of starch, and utilization of histidine. The strains of group A were all positive and those of group B all negative for them.
4) Only the strains of group A produced an antibacterial substance which characteristicallyinhibited the growth of Gram-positive bacteria but not that of Gram-negative ones.View full abstractDownload PDF (3414K) -
Masaaki HAYAMA1980 Volume 35 Issue 4 Pages 639-649
Published: July 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe induction of delayed type hypersensitivity was demonstrated clearly in mice immunized with viable group A streptococci. Female BALB/c mice were inoculated subcutaneously with 1×107 viable cells weekly for 8 weeks and used for experiments 7-10 days after the last immunization. The streptococci inoculated in mice were group A streptococcus pyogenes types 3 and 12 and non-typable strains and group D streptococci.
Positive foot pad reactions were observed with homologous and heterologous antigens. Reactivity was transferred by immune lymphocytes, but not by ordinary lymphocytes, from normal mice or by sera from infected mice. The infiltration of mononuclear cells was observed among histological changes caused by the foot pad reaction. An increase was demonstrated in 3H-TdR incorporation by lymphocytes obtained from infected mice when homologous and heterologous antigens were added to the cells. When the cells were treated with anti-thymocyte serum and complement, the proliferative response and passive transfer were remarkably reduced in the foot pad reaction.
Crude antigens were prepared from streptococcal cell walls. They were further fractionated by hydroxyl apatite column chromatography and stepwise elution with 0.01, 0.1 and 0.3M sodium phosphate, pH 6.8. The resulting fractions were designated P-1, P-2 and P-3, respectively. All of them, but P-3, showed homologous and heterologous responses in 3H-TdR incorporation. P-3 showed only a homologous response in 3H-TdR uptake. It was suggested that two kinds of cell wall protein might have appeared to cause delayed type hypersensitivity.View full abstractDownload PDF (1641K) -
Hidenobu OKAZAKI, Toshiyuki KOOZAI, Shigeki YAMANISHI, Tadao YAMAMOTO, ...1980 Volume 35 Issue 4 Pages 651-652
Published: July 25, 1980
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (219K)
- |<
- <
- 1
- >
- >|