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Volume 24, Issue 5
Displaying 1-6 of 6 articles from this issue
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1. Phagocytic Activity of Rabbit AIveoIar Macrophage on Various MicroorganismsKensuke Ozu1969 Volume 24 Issue 5 Pages 221-227
Published: May 25, 1969
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSMacrophages collected from the rabbit lung by the method of Myrvic showed a high degree of uniformity both morphologically and functionally; more than 95% of the cells consisted of large monocytes and 90 to 95% of the cells had an activity of phagocytosis in vitro.
A study on the phagocytosis of these cells against Staphylococcus epidermidis revealed that the phagocytic action required normal rabbit serum as an active component in the reaction mixture, and that when added at the rate of 10%, the serum exhibited a full activity. The active component contained in normal rabbit serum was found to react directly with Staphylococcus epidermidis by opsonization so that the organism might easily be picked up by the macrophages. No washing of the opsonized organism exerted influence on the phagocytic index of the macrophages. The pretreatment of the macrophages with the active component enhanced the phagocytic action of these cells to some extent, but far less than the same treatment of the microorganism.
Kinetic experiments made on this system showed that the phagocytic reaction apparently proceeded on the basis of the first order reaction, and that the time required for 50% reaction was 14 minutes in a given conditionView full abstractDownload PDF (874K) -
Activities of Extracellular Enzymes in Culture FiltrateKiyoaki KAMIJO, Sutemi INOUE1969 Volume 24 Issue 5 Pages 228-233
Published: May 25, 1969
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSStudies were carried out on 3 staphylococcal strains with special reference to the α- and β-hemolysin, coagulase, lysozyme, and deoxyribonuclease activities in the fractions of their concentrated culture filtrates. The strains used were the Terashima, E-97 and E-46. The virulence and some biological characteristics of them were described in the previous report. Each strain was cultured in modified Chesbro's medium, from which a filtrate was obtained at 72 hours of incubation at 37°C. Results are summarized as follows.
1) The coagulase and lysozyme activities were reliable parameters of the virulence, but the production of hemolysin, both α- and β-hemolysin, was not.
2) Coagulase activity was not observed in strain E-97 or E-46 by the ordinary method, but was shown markedly in concentrated culture filtrates of both strains.
3) Deoxyribonuclease activity was distinct in strains Terashima and E-97, but not so marked in strain E-46, though strains E-97 and E-46 were almost equal in virulence.
4) The coagulase activity was always seen in fraction I in all the cases, suggesting that the essential nature of the enzyme might be the same in all the strains. The other enzyme activities appeared in fraction I in some cases and fraction II in another, suggesting that the nature of these enzymes might be different from one strain to another.View full abstractDownload PDF (590K) -
3. Skin Reaction and Intracutaneous Staphylococcal Infection of Rabbits Previously Treated with the Live OrganismYoshio Sawai, Takuya Ishii, Akira Nozawa, Masashi Watanabe1969 Volume 24 Issue 5 Pages 234-239
Published: May 25, 1969
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSAfter injected intramuscularly with living cells of 2 given strains of Staphylococcus aureus, rabbits were observed for the skin reaction and behavior to intracutaneous infection with the same organism.
The sensitized rabbits showed acceleration of the skin reaction. Three weeks after the sensitization, they were challenged intracutaneously by the same staphylococcal strain. In these rabbits, the redness around the abscess was accelerated, whereas the abscess was absorbed more quickly, without showing any ulceration, than in unsensitized controls. A decrease in local reaction and acceleration of recovery occurred in the sensitized rabbits after intravenous injection with vaccine or toxoid.
These results suggested that the effect of staphylococcal toxoid might be derived not from the antitoxic effect but from the desensitizing capacity.View full abstractDownload PDF (906K) -
Purification and Protein Extraction with Phage P22Kiyoaki KAMIJO, Michiko HORIKOSHI1969 Volume 24 Issue 5 Pages 240-245
Published: May 25, 1969
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSPhage P22 was purified by the concentration in vacuo, ultracentrifugation and ECTEOLA-cellulose column chromatography from its lysate. Yield of the purified Phage was 10ml (1013 phages per ml) obtained from original lysate of 3l. The protein preparation was carried out by acetic acid degradation and its yield was 10ml (2.2mg/ml) from 50ml of the purified phage. The protein showed the mobility of 8.293mm in the electrophoresis on cellulose acetate strip, while the mobilities of the components of rabbit serum were 23.771, 16.271, 10.771 and 8.443mm, respectively. The values of the host flagella and host protein were 3.650mm and 0.000mm, respectively. Consequently, the phage protein obtained was of a single component and differed from the host protein. According to the amino acid analysis, histidine, leucine, lysine, and phenylalanine were found more abundantly in phage protein than in host protein. In general, alanine, aspartic acid, glutamic acid, glycine and leucine were predominant of the other amino acids. Proline was found only in the phage protein and was not in the host protein.View full abstractDownload PDF (765K) -
Keiji OGIMOTO, Shigeo NAMIOKA, Tsuneji SUTO1969 Volume 24 Issue 5 Pages 246-251
Published: May 25, 1969
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSSerological grouping of Veillonella alcalescens strains isolated from the alimentary tract was performed by cross agglutination. There were complicated antigenic relationships among these strains, which were divided basically into two groups. One group consisted of strains of rumen origin and the other of strains of lower alimentary tract origin.
When Veillonella alcalescens was examined for antigenic structure, it possessed heat-labile antigen in addition to somatic antigen. Furthermore, it was clarified that formolized antigen was closely related to heat-labile antigen. This heat-labile antigen corresponded to K-antigen of enterobacteria. It resembled C-type antigen found in Proteus, but did not resemble B-type antigen of Neisseria gonorrhoeae.View full abstractDownload PDF (651K) -
1969 Volume 24 Issue 5 Pages 252-256
Published: May 25, 1969
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (712K)
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