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Volume 36 (1981)
- Issue 6 Pages 719-
- Issue 5 Pages 663-
- Issue 4 Pages 617-
- Issue 3 Pages 557-
- Issue 2 Pages 445-
- Issue 1 Pages 42-
Volume 36, Issue 6
Displaying 1-11 of 11 articles from this issue
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Takushi TADAKUMA, Tatsuji YASUDA1981 Volume 36 Issue 6 Pages 719-724
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
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Masahiro NAKAMURA1981 Volume 36 Issue 6 Pages 725-740
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
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Yoshiki UCHIDA, Tsutomu NISHIHARA, Tomio ICHIKAWA, Masaomi KONDO1981 Volume 36 Issue 6 Pages 741-745
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSWhen treated with p-chloromercuribenzoate (PCMB), spores of Bacillus megaterium ATCC 19213 were changed to be able to germinate with glucose. When further treated with 8M urea, the PCMB-treated spores lost their ability to germinate with glucose. The ratio of germination reaction to alanine-inosine solution remained high after the two treatments. In the urea treatment, 50% of the mercury contained was released from the PCMB-treated spores.
The urea extract of the PCMB-treated spores exhibited at least two fractions of mercury by Sephadex G-25 column chromatography. The greater portion of the mercury contained was found in one fraction consisting of low molecular compounds which were ninhydrin-positive, and the remaining portion in the other fraction consisting of high molecular compounds. These results suggest that the PCMB-bound components eluted in the extract may play an important role in a specific reaction of spores against germinants.View full abstractDownload PDF (596K) -
GC-MASS AnalysisYoji NAKAGAWA1981 Volume 36 Issue 6 Pages 747-755
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSTwo types of diacetylated chloramphenicol 1, 3-O-O-di [1-12C] acetyl chloramphenicol and 1, 3-O-O-di [1-14C] acetyl chloramphenicol, were subjected to GC-mass analysis. The former showed the presence of prominent mass peaks at m/e 195, 212, 214 and 216, and the latter at m/e 197, 214, 216 and 218. Thus, the fragment ions of the compound diacetylated with. [1-14C] acetyl CoA were detected at a higher mass field by m/e 2 than those of the one diacetylated with [1-12C] acetyl CoA. The C1-C2 bond of diacetylated chloramphenicol was subjected to cleavage. The mass peaks at m/e 195 and 197 and those at m/e 212, 214, 216 and 218 were produced by the fragment containing C-1 and that containing C-3, respectively. The technique was applied to identify the acetoxy group attached to C-1 or C-3 of diacetylated chloramphenicol.
To clarify the exact position acetylated by chloramphenicol acetyltransferase in chloramphenicol, the [1-14C] acetyl group wes introduced into 3-O-[1-12C] acetyl chloramphenicol, by using [1-14C] acetyl CoA. The mass peaks of diacetylated chloramphenicol appeared at m/e 195, 212, 214, 216 and 218. Especially, the relative intensity at m/e 214 increased. No peaks occurred, however, at m/e 197. The result described above indicated that the [1-12C] acetyl group was attached to C1-OH and the [1-14O] or the [1-12C] acetyl group to C3-OH in chloramphenicol.
From these result and those of kinetic studies, it was considered that both mono- and diacetylation or chloramphenicol by chloramphenicol acetyltransferase might take place in the hydroxy group attached to C-3 of chloramphenicol.
The presence of the [1-14C] acetyl group in monoacetylated chloramphenicol suggested a mutual exchange between the [1-12C] acetyl and the [1-14C] acetyl groups.View full abstractDownload PDF (3177K) -
Takayoshi YAMAGISHI, Kenichi KONISHI, Kenichi SAKAMOTO, Shinya SAKURAI ...1981 Volume 36 Issue 6 Pages 757-765
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe fate of Clostridium perfringens was observed in the intestine of guinea pigs having ingested this organism orally. Vegetative cells of C. perfringens ingested were found in the feces between 4 and 15hr later, but not 24hr later. From the number of these cells contained in the feces it was suggested that C. perfringens might not have proliferated in the intestine. When spores of this organism were administered to guinea pigs, they appeared in the feces 3hr later and were detected for 4 days, but disappeared on the 5th day after administration. The ratio of heat-resistant cells to total cells found in the feces indicated that some of the spores germinated but did not proliferate in the intestine.
From experiments on the survival of the organism in a medium made from the intestinal juice, it was suggested that the bacterial flora and some substance(s) in the juice, which were filtrable through Millipore filter but destroyed by heating, might kill C. perfringens or inhibit the growth of it. This organism was detected from the ileum, colon and rectum of guinea pigs 24hr after administration with its spores, but not from any part of the intestine of control animals. In guinea pigs given spores of C. perfringens no marked changes were observed in any constituent of the intestinal flora, except C. perfringens.View full abstractDownload PDF (1180K) -
(10) Role of Surface Metals in Germination of Bacillus megaterium SporesTsutomu NISHIHARA, Yoshie KANOH, Yoshiki UCHIDA, Masaomi KONDO1981 Volume 36 Issue 6 Pages 767-774
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSWhen spores of Bacillus megaterium QM B1551 were incubated in acidic buffer containing citric acid, they released such surface metals as Ca, Mg, K, Mn and Fe, and were converted to the hydrogen form (H-spore) which could not germinate with KNO3 plus glucose. The loss of germinability was correlated with the amount of Ca released during the treatment. The germinability of the H-spore was recovered by the subsequent treatment with cations, of which divalent cations, especially Ca ions, were more effective then monovalent cations. These phenomena occurred reversibly when the treatment was repeated. Electron probe x-ray microanalysis of ashed Sr-spores showed that Sr was distributed only in the coat, while Ca was in the coat and the core as that of ashed native spores showed. Ca-spores had an increased germinability to germinants, especially to KNO3. These results suggest that metals, especially Ca, in the coat, which are exchangeable, may play an important role in the initiation of ionic germination.View full abstractDownload PDF (3568K) -
Hirobumi MIYOSHI, Toshirou UESAKA, Kouichi SANO, Yasumori TAKAYAMA, Ma ...1981 Volume 36 Issue 6 Pages 775-777
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
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Glucose Phosphotransferase SystemShiro IUCHI, Susumu KIMURA, Shuji TANAKA1981 Volume 36 Issue 6 Pages 779-780
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
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Masanori ISHIBASHI, Yoshio KINOSHITA, Yoshiaki YANAI, Hisao ABE, Yoshi ...1981 Volume 36 Issue 6 Pages 781
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
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Akira TAMURA, Hiroshi URAKAMI1981 Volume 36 Issue 6 Pages 783-785
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
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1981 Volume 36 Issue 6 Pages 787-797
Published: November 25, 1981
Released on J-STAGE: February 19, 2009
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