Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 45, Issue 5
Displaying 1-6 of 6 articles from this issue
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  • Molecular genetic studies on the capsule plasmid of Bacillus anthracis
    Ikuo UCHIDA, Sou-ichi MAKINO
    1990 Volume 45 Issue 5 Pages 821-832
    Published: September 25, 1990
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.45.821
    JOURNAL FREE ACCESS
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  • Asymptomatic infection of Legionella pneumophila in four cases with pulmonary diseases
    Hajime FUKUNAGA, Katsumi AKAGI, Eiko YABUUCHI
    1990 Volume 45 Issue 5 Pages 833-840
    Published: September 25, 1990
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.45.833
    JOURNAL FREE ACCESS
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    In view of the wide-spread existence of legionellae in cooling-tower and other environmental water, asymptomatic infection of this organism could occur. In order to verify the possibility of colonization of legionellae at lower respiratory tract of patients with various pulmonary diseases, a total of 22, 036 sputum samples from in- and out-patients at National Sanyoso Hospital were examined during a five-year period from September, 1984 to August, 1989. Four (0.073%) out of 5, 502 cases were culture-positive for L. pneumophila. L. pneumophila strains were isolated from expectorated, subsequently washed sputum samples of two male and two female patients with respiratory tract diseases. The identification of the isolates was genetically confirmed by the fluorometric microplate DNA-DNA hybridization method. The serogroup (SG) and viable counts of L. pneumophila per ml of sputum of each patient were as follows: 73 y/o female K.H., SG-6, 103 CFU; 75 y/o male H.J., SG-5, 104 CFU; 61 y/o female M.S., SG-5, 105 CFU; and 77 y/o male M.G., not-agglutinable against SG-1∼6 antisera, 104 CFU. None of the four patients was clinically suspected of legionellosis and antibody titer of paired sera remained 1:64 or lower than 1:32. From these findings, we concluded that L. pneumophila can cause, though quite rarely, asymptomatic infection in human respiratory tract. None of the environmental samples obtained from in- and out-side of the Hospital was culture-positive for legionellae. Thus, the source of infection has remained unknown.
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  • Spore-Spheroplast Transformation of Bacillus megaterium
    Yoshihiro TAKUBO, Junichi NISHIKAWA, Katsuji TANI, Tsutomu NISHIHARA, ...
    1990 Volume 45 Issue 5 Pages 841-843
    Published: September 25, 1990
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.45.841
    JOURNAL FREE ACCESS
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    A new method for transformation of Bacillus megaterium was developed by modification of Chang and Cohen's method. In our method, spore spheroplasts were used as recipent cells instead of the protoplasts of vegetative cells. Longer incubation (60min) of spore spheroplasts and plasmid DNA before treatment with polyethylene glycol remarkably increased the efficiency of transformation. The frequency of transformation was about 104 per μg of plasmid DNA. A shot-gun-type cloning of chromosome DNA of B. megaterium ATCC 12872 was available in B. megaterium ATCC 19213 strain by this transformation method.
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  • Induction by Staphylococcus aureus L-form of tumor necrosis factor-alpha from macrophages
    Ikuko MATSU-URA, Koichi KUWANO, Akiko UMEDA, Kazunobu AMAKO, Yasumichi ...
    1990 Volume 45 Issue 5 Pages 845-849
    Published: September 25, 1990
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.45.845
    JOURNAL FREE ACCESS
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    Induction of tumor necrosis factor-α (TNF-α) by Staphylococcus aureus L-form was investigated. The supernatant of a macrophage culture mixed with S. aureus L-form showed a potent cytotoxic activity to L cells. Addition of anti TNF-α antibody inhibited completely the cytotoxic activity of the supernatant, indicating that the activity might be due mostly to TNF-α. To investigate localization of TNF-α production, the membranes of hypotonicity treated L-form were layered on a step-gradient composed of an upper and lower layers of 35% and 50% sucrose, respectively. The membranes were banded at the interface of 35% and 50% of sucrose. The activity of TNF-α production of the membrane fraction was 10-times higher than that of the soluble fraction.
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  • Quantitative microdilution plate hybridization to determine genetic relatedness among baterial strains
    Takayuki EZAKI, Shatha ADNAN, Masami MIYAKE
    1990 Volume 45 Issue 5 Pages 851-857
    Published: September 25, 1990
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.45.851
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1990 Volume 45 Issue 5 Pages 859-864
    Published: September 25, 1990
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.45.859
    JOURNAL FREE ACCESS
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