The participation of sporangial cytoplasm in dipicolinic acid biosynthesis was studied. When lysozyme was added to sporulating culture of B. subtilis at various stages, the further synthesis of dipicolinic acid was immediately and almost completely inhibited. It was also found that, when isolated forespores were incubated in culure supernatant obtained from sporulating culture, dipicolinic acid was not synthesized. The changes in the distribution of the diketopimelate oxidation system in cells were studied. The oxidative activity was found in the soluble fraction, and not in the residue fraction during all stages of sporulation. The activity was not detected in mechanical disintegrates of forespores and also in lysozyme lysates of the vegetative cells, and the activity suddenly emerged just before the onset of sporulation. The active substance was supposed to be low molecular organic substance by qualitative analyses. From the above results, it was concluded that the cytoplasm surrounding the developing spore is required for dipicolinic acid synthesis.
A series of studies has been conducted about the antigenic structure of the Reiter Strain of Treponema pallidum using a refined Reiter protein antigen purified by lower phase solvent adapting the Agar Gel Diffusion test and Complement Fixation test. Through these studies, the existence of the common antigen between the Reiter strain and pathogenic treponema (Nichols strain) was observed and furthermore this chemical component was acknowledged as the protein. However, the removal of appearance of the nonspecific reaction was nearly impossible at the RPCF test using this purified Reiter antigen. This should be considered, as the author has refered previously, to be caused by the common antigen of the saprophytic treponema existing in the healthy human. On the other hand, the positive reaction was observed at the serologic test for the human serum from the syphilitic individual absorbed by the Reiter strain using the pathogenic treponema (Nichols strain) as an antigen, and from this results, it was found the Reiter strain has only the common antigen which is exist among treponemes except own specific antigen, and the pathogenic treponema has the specific antigen other than the Reiter strain. From the test aforementioned the usage of the Reiter strain as an antigen at Complement Fixation test is not appopriate, and rather it should be used as an absorption antigen for nonspecific reaction caused by the common antigen among treponemes at the test involving the Fluorescent Treponemal Antibody test where the pathogenic treponema is being used as an antigen.
Three hundred sixteen strains of mycobacteria, including tubercle bacilli, other acid-fast pathogens, and several saprophytic mycobacteria were examined for the presence a 70°C heat stable acid phosphatase. The acid phosphatase test, devised by the authors, was performed on filter paper, using the “SM TEST” (Ishizu Pharmaceutical Co., Ltd.) and 10 minute incubation time at 21-25°C. Among the slowly growing mycobacteria, M. kansasii gave a positive reaction, whereas none of mammalian tubercle bacilli, M. avium and M. intracellulare gave positive reaction. It is worthy of note that, among the Group III mycobacteria, all strains of M. gastri, Subgroup “V” and most of M. terrae and M. novum were positive to this test. These finding are helpful for differentiating M. avium and M. intracellulare from Group III saprophytes. Among the rapidly growing acid-fast bacilli, M. fortuitum and M. marinum, which gave positive reactions may be distinguished from the others, which gave negative reactions. This test is one of the differential keys between M. fortuitum and M. runyonii, which are similar in many biological and biochemical properties, as well as the pathogenicity for mice. This test for acid phosphatase determination is rapidly and easily carried out in the laboratory. The results of the test, except in rare cases, were not ambiguous and reproducible in all cases. For these reason, it is felt that the method provides an additional biochemical criterion to identify and classify various strains of mycobacterium.
A strain of Corynebacterium was isolated from a patient suffering from unknown febrile disease which persisted for about 4 months. The strain, named Saito 60422, was identified as Corynebacterium pyogenes according to the morphological, cultural and pathogenical characteristics. Aggultination reaction was positively performed with the strain and the patient serum even in dilution of 1:1, 280, suggesting that the strain was an etiological agent of the disease. Concerning the antibiotic-resistance, the strain was slightly sensitive to only sulfathiazole and resistant to chloramphenicol, tetracycline, streptomycin and penicillin.