Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 47, Issue 6
Displaying 1-5 of 5 articles from this issue
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  • Active site of heat-labiel enterotoxin (LT) produced by enterotoxigenic Escherichia coli
    Takao TSUJI
    1992 Volume 47 Issue 6 Pages 729-745
    Published: November 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.729
    JOURNAL FREE ACCESS
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  • Significance of endogenous cytokines in host defense against Listeria monocytogenes
    Akio NAKANE
    1992 Volume 47 Issue 6 Pages 747-757
    Published: November 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.747
    JOURNAL FREE ACCESS
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  • Newly developed MRSA medium
    Fumiaki TAGUCHI, Tatsuo SAITO-TAKI, Shunji OKUDA, Ritsuko KIKUNO
    1992 Volume 47 Issue 6 Pages 759-765
    Published: November 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.759
    JOURNAL FREE ACCESS
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    We have developed a new selective medium, tentatively named MR(SA)2, for rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) from clinical specimens. MR(SA)2 medium contained modified Müller-Hinton agar supplemented with 75g of NaCl, 10g of mannit, 20mg of bromcresol purple, 20g of egg-yolk, 4mg of oxacillin, and 12.5mg of ceftisoxime per 1, 000ml. There were no differences between the growth of MRSA strains on this medium at 30C and that of 37C. This medium can detect the egg-yolk reaction instead of the coagulase reaction. By single streaking of a test material on the surfaces of MR(SA)2 agar, MRSAs can easily be distinguished from methicillin-resistant coagulase-negative staphylococci (MR-CNS), other bacteria and fungi from their colony morphologies in a quantitative manner. A few MRSA strains would not form colonies on this medium because of their susceptibilities to ceftisoxime, but this may not inpede its use, since most MRSA strains isolated from clinical materials showed resistance to ceftisoxime. From the above results, the MR(SA)2 medium may be suituable for rapid detection of MRSA and MR-CNS.
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  • Proposal for the Nosocomial Infection Control of Methicillin- Resistant Staphylococcus aureus (MRSA)
    F. TAGUCHI, T. SAITO-TAKI, S. OKUDA, M. AOKI, T. MATSUZAKI, M. TOMIOKA ...
    1992 Volume 47 Issue 6 Pages 767-775
    Published: November 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.767
    JOURNAL FREE ACCESS
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    To determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in hospital environments, quantitative isolation of MRSA from environmental and human specimens was performed.It was found that as many as 6×104 colony forming units (CFU)/100cm2 of MRSA and also methicillin-resistant coagulase-negative Staphylococcus (MRCNS) were isolated from 1) 25∼100% of the bed-mattresses tested in 10 out of 11 (90%) hospitals, and also 2) bed sheets for patients, floors of patient wards, laundry, bath-room, toilet and laundry storage-room. And seven and eight palms of 20 patients were contaminated with MRCNS and MRSA, respectively, and MRCNS contamination was revealed in six of medical staffs. These results indicate that hospital environments, especially the mattresses and hospital floors are highly contaminated with MRCNS and MRSA, and sanitation and cleanliness of mattresses and floors are necessary to prevent the dissamination of both MRCNS and MRSA in hospital.
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  • Chemical and serological study of lipopolysaccharide isolated from Shigella flexneri 88-893 possessing a new type-antigen
    Yumiko TOSHIMA, Takehiro IGUCHI, Seiichi KONDO, Shigeru MATSUSHITA, Ya ...
    1992 Volume 47 Issue 6 Pages 777-781
    Published: November 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.777
    JOURNAL FREE ACCESS
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    The O-specific polysaccharide chain which represents a new type-antigen in lipopolysaccharide (LPS) of Shigella flexneri 88-893 was investigated. The O-polysaccharide chain was found to be composed of repeating units comprising rhamnose, N-acetylglucosamine and glucose (3:1:2). In the passive hemolysis test, group-6 antiserum of S. flexneri exhibited a high hemolytic titer (50% hemolysis titer: 7, 900) against sheep red blood cells (SRBC) sensitized with intact 893 LPS, but virtually no hemolytic activity against SRBC sensitized with alkali-treated 893 LPS. None of the type-specific antisera (I-VI), showed any significant hemolytic titer against SRBC sensitized with either intact or alkali-treated 893 LPS. Thus, 893 LPS contained both the group-6 antigen and a new type-antigen which is distinct from any known type-antigen of S. flexneri.
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