All issues
Volume 32 (1977)
- Issue 6 Pages 777-
- Issue 5 Pages 655-
- Issue 4 Pages 533-
- Issue 3 Pages 455-
- Issue 2 Pages 337-
- Issue 1 Pages 31-
Volume 32, Issue 5
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Yoetsu HACHISUKA1977 Volume 32 Issue 5 Pages 655-669
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2396K) -
Ryosuke MURATA, Akira OHSAKA1977 Volume 32 Issue 5 Pages 671-689
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (7926K) -
I. Enhancement of Phagocytic and Bactericidal FunctionsToshimasa NITTA, Seiichi OKUMURA, Masayasu NAKANO1977 Volume 32 Issue 5 Pages 691-696
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSBacterionema matruchotii was examined for enhancing effects on the phagocytic function of the reticuloendothelial system and the bactericidal activity of peritoneal macrophages in mice by the carbon clearance test and the viable count of infecting salmonellae.
In mice injected intraperitoneally with 1mg of B. matruchotii, the clearance-rate of carbon in the circulating blood was increased significantly 48 hours after injection. Of three strains tested, the L2 strain had the highest, the #13 strain the second highest, and the ATCC 14266 strain the lowest ability to stimulate the phagocytic function in mice. Injection with 125 to 2, 000μg of the L2 strain could enhance the reticuloendothelial function of mice. The larger the dose injected, the higher the clearance-rate. In mice injected with 1mg of L2 strain, the clearance-rate was maintained at an increased level over a period from 6 hours to 20 days after injection. Especially, 2 and 7 days after injection, this rate was increased to a maximum. Either viable or heat-killed organisms of the L2 strain were capable of stimulating the phagocytic function of mice.
When injected intraperitoneally with 1mg of the L2 strain, mice showed a higher bactericidal activity over infecting organisms of Salmonella typhimurium LT2 than uninjected control mice 2 and 7 days after injection.
Peritoneal macrophages collected from them were also capable of killing infecting organisms of S. typhimurium LT2 in vitro. It was suggested that these macrophages might have acquired bactericidal capacity from the previous injection of B. matruchotii into those mice.View full abstractDownload PDF (824K) -
I. Fatty Acid Composition and Distribution in Dormant and Germinated Spores of Bacillus subtilisHiroo ISHIHARA, Hideo NAGANO, Tsutomu NISHIHARA, Masaomi KONDO1977 Volume 32 Issue 5 Pages 697-702
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDormant spores, germinated spores, and their subcellular fractions of Bacillus subtilis NRRL B558 were examined for the fatty acid composition by gas liquid chromatography. The lipid contents were 7.8% in dormant spores and 5.8% in germinated spores. More than 95% of the fatty acids consisted of saturated and branched-chain fatty acids, such as iso C15:0, anteiso C15:0, and iso C17:0 acid. Of them, 85% was odd-numbered and 15% even-numbered. The fatty acid composition, as well as lipid contents, was similar between dormant and germinated spores. Fatty acids were recovered almost always from the coat and core fractions. As for the fatty acid composition in the coat fraction of dormant spores, anteiso, iso, and normal acids were 42%, 55%, and below 3%, respectively. In the core fraction, iso acids were predominant, or 63%, and normal acids only 4%. After germination, the fatty acid composition did not so much change in each fraction. The amount of anteiso acids increased in the core fraction and decreased slightly in the coat fraction.View full abstractDownload PDF (576K) -
II. Changes in Fatty Acid Composition of Bacillus subtilis Cells during Growth and SporulationHiroo ISHIHARA, Hideo NAGANO, Tsutomu NISHIHARA, Masaomi KONDO1977 Volume 32 Issue 5 Pages 703-707
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSBacillus subtilis NRRL B558 cells were examined for changes in fatty acid composition during growth and sporulation by the aid of gas chromatography. C13-C18 acids were detected throughout the period of growth and sporulation, and iso C15:0, anteiso C15:0, iso C17:0, anteiso C17:0 and iso C16:0 were major components. More than 80% was odd-numbered, saturated and branched-chain acids, which were characteristic of this strain. Changes in amount of fatty acids, such as iso C15:0, anteiso C15:0, and iso C16:0, during the stage of sporu-lation were detected. They might be explained either by the difference in biosynthetic pathway between the mother cell and forespore fractions, or by selective transport to and accumulation in the forespore part.View full abstractDownload PDF (445K) -
III. Composition of Lipids in Vegetative Cells and Spores of Bacillus subtilisHiroo ISHIHARA, Hideo NAGANO, Tsutomu NISHIHARA, Masaomi KONDO1977 Volume 32 Issue 5 Pages 709-714
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe composition of lipids was compared between the vegetative cells and the spores of of Bacillus subtilis NRRL B558. Neutral lipids, phospholipids, and glycolipids were detected at the rate of 25%, 70%, and 5%, respectively, in the vegetative cells, and 43%, 56%, and 1%, respectively, in the spores. In the vegetative cells, cardiolipin, phosphatidyl glycerol, phosphatidyl ethanolamine, and unidentified ninhydrin-positive lipids were found as phospholipids. In the spores, cardiolipin was a major component of phospholipids (more than 70%) and the latter two lipids were minor ones. Hydroxy-fatty acid ester was found as one of the neutral lipids, and diglucosyl diglyceride demonstrated as a minor component in the vegetative cells.
These results suggested that the alteration of lipid metabolism might have induced physiological and morphological changes of the organism during the stage of sporulation.View full abstractDownload PDF (493K) -
1977 Volume 32 Issue 5 Pages 715-724
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1515K) -
1977 Volume 32 Issue 5 Pages 725-741
Published: September 25, 1977
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2752K)
- |<
- <
- 1
- >
- >|