Nippon Saikingaku Zasshi

Rencent Advances in the Isolation and Identification of Pseudomonas aeruginosa and Related Gram-negative Rods

Sachiko GOTO, Shogo KUWAHARA, Taketoshi ARAI

1972 Volume 27 Issue 5 Pages 621-627
Published: September 25, 1972
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.27.621

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Studies on Pseudomonas aeruginosa Infection and Its in the Future

J.Yuzuru HOMMA

1972 Volume 27 Issue 5 Pages 629-640
Published: September 25, 1972
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.27.629

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Studies on Pseudomonas aeruginosa

I. Isolation of P. aeruginosa in Nature and Animals

Bunzo NOMIYA, Katsumi KAWAHARAJO, Tadahiro WATANABE, Sanae TAMURA

1972 Volume 27 Issue 5 Pages 641-648
Published: September 25, 1972
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.27.641

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In order to develop a suitable method of evaluation of chemotherapeutic agents against Pseudomonas aeruginosa, it is necessary to know a detection rate of this organism from healthy experimental animals maintained under laboratory conditions. In the present study, an attempt was made to isolate this organism from mice and guinea pigs, as well as dogs, cattle, and such natural materials as soil and water. The results obtained are summarized as follows.
1. The selectivity of NAC agar for P. aeruginosa (the number of P. aeruginosa strains/ the total
number of bacterial strains grown on the medium) was 85.7 to 100% in the case of specimens from such animals as mouse, guinea pig, dog, and cattle, and 12.5 to 44.7% in the case of samples from soil and water of pond, sewage, basin, ditch, etc.
2. A total of 5, 646 specimens were examined for the presence of P. aeruginosa. They consisted of 64 soil and 64 water samples, 5, 176 specimens from the nasal cavity, mouth, anus, and intestine of mice and guinea pigs, 168 specimens from the mouth and anus of dogs, 113 milk samples, and 61 pathological specimens of animals. The isolation rate of the organism was 26.3% for the guinea-pig mouth, 32.8% for water, 18.3% for the guinea-pig intestine, 13.6% for the stray-dog anus, 9.1% for the stray-dog mouth, 7.8% for soil, 3.5% for the mouse intestine, 2.0% for milk, 1.4% for the mouse mouth, 1.3% for the guinea-pig anus and 1.0% for the mouse anus.
3. When weekly examination was carried out over a period of 12 weeks, P. aeruginosa was detected sporadically from both mouth and anus of 1.0 to 3.0% of 100 mice. The organism was isolated constantly from the mouth of 16.0 to 32.0% and sporadically from the anus of 1.0 to 4.0% of 100 guinea pigs studied.

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Studies on the Toxin of Aspergillus fumigatus

III. Fraction Causing Dermonecrosis and Hemorrhage-Like Changes

Osamu SAKAGUCHI, Katsushi YOKOTA

1972 Volume 27 Issue 5 Pages 649-655
Published: September 25, 1972
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.27.649

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Investigation was made on the dermonecrotic activity of crude toxin of Aspergillus fumigatus and the hemorrhagic activity of a fraction isolated from the toxin by column chromatography. The dermonecrotic activity and heat stability of the toxic fraction were in parallel with the activity of protease, such as caseinase, gelatinase, and collagenase.
Neutralization of dermonecrotic activity with antitoxin serum resulted in the disappearance of proteolytic activity. Similar inactivation was seen in the toxic fraction by treatment with urea or monoiodoacetic acid.
When partial purification was done by DEAE-cellulose column chromatography, a fraction of sugar-containing protein with a low lethality was fractionated from the crude toxin. It was found to possess an activity to induce dermonecrosis and especially hemorrhage, without causing hemolysis.

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Studies on Staphylococcal L-Forms

I. Induction and Morphological Characteristics of Staphylococcal L-Forms

Tohru EDA, Shunsuke MATSUOKA, Ichiro TADOKORO

1972 Volume 27 Issue 5 Pages 657-664
Published: September 25, 1972
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.27.657

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Fifty strains of Staphylococcus aureus isolated from clinical specimens were examined for the induction of L-forms by the penicillin-disc technique. They were cultivated on L-form medium which was composed of brain-heart infusion broth, 5% NaCl, 1% special agar, and 15% horse serum. L-forms were successfully induced from a strain, Tasaki, which had been isolated from a patient with ear infection.
The staphylococcal L-forms thus induced were designated as STA-EMT-1 strain. L-colonies were detected initially in five to seven days. After several transfers on L-form media they appeared in a few days, exhibiting a typical, so-called “fried-egg” configuration on L-form medium. Microscopically, the L-colony consisted of dark central granular elements and light peripheral spherical cells, ranging from several to more than ten microns in diameter.
Staphylococcal L-forms could not grow on blood agar plate or any other medium routinely used for bacteria, but could easily be transferred to a fresh L-form medium by means of the agar block technique. They appear to be of stable type. No reversion to the parent bacterial form has occurred to them until the present time after about thirty transfers on L-form media without addition of penicillin.

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[title in Japanese]

1972 Volume 27 Issue 5 Pages 665-667
Published: September 25, 1972
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.27.665

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