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Volume 21, Issue 9
Displaying 1-7 of 7 articles from this issue
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Kazuo IWATA, Tsunehiko HIRATA1966 Volume 21 Issue 9 Pages 533-540
Published: September 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSMitochondria and intracytoplasmic membrane system of the yeast-like cells of Candida albicans were demonstrated by electron microscopy.
Fixation of the materials was done in a 2% aqueous solution of potassium permanganate for 48 hours at room temperature and for embedding was used a methacrylate mixture.
The mitochondria with cristae mitochondriales were surrounded by a continuous membrane and its profiles were surrounded by a continuous membrane and its profiles were circular or ellipsoidal. The cristae seemed to be formed by infolding the inner part of the limiting membrane.
The intracytoplasmic membrane system or the endoplasmic reticulum appeared to be closely connected with not only the cytoplasmic membrane but also the nuclear membrane.
These membrane systems consisted of three layers, at least; two electron dense layers and a less dense interspace. Each one was measured 35-50A in width.View full abstractDownload PDF (12112K) -
The Relationship Between the Chemical Structure of Trimethylammonium Derivatives and the Biological Action on the Multiple-drug-resistance TransferKohtaku HAYASHI, Tomiko KODAIRA, Kazuko KIKUCHI, Kumiko BABA1966 Volume 21 Issue 9 Pages 541-551
Published: September 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSTwenty three trimethylammonium (TMA) derivatives were tested for their action on the multiple-drugresistance (R) transfer from the R-resistant Shigella flexneri 3a strain MZ 17R to the sensitive E. coli strain K-12 C25S, and also on the inverse R-transfer from the R-resistant E. coli K-12 C17R to the sensitive Shigella flexneri 3a MZS.
Among the compounds tested, dodecyl-TMA, myristyl-TMA, cetyl-TMA, octadecyl-TMA, dodecylbenzyl-TMA, myristoyl-choline and benzethonium chloride inhibited the both R-transfer in a concentration of 10∼40mcg/ml, and they also showed some antibacterial activity. By a new evaluation method excluding the antibacterial activity, they were regarded to have the specific actions on the R-transfer.
Although propyl-TMA, butyl-TMA, pentyl-TMA, hexyl-TMA, octyl-TMA and benzoyl choline did neither inhibited the growth of Shigella and Escherichia, nor accelerated the growth of them, put these compounds accelerated the multiple-drug-resistance transfer from MZ 17R to C25S were observed.,
As for the relationship between the chemical structure and the biological action in a serial compounds of alkyl-trimethylammonium halogenides, the following fact was worth notice.
The compounds holding from 3 to 8 carbons in the side chain have an accelerating effect on the multiple-drug-resistance transfer and these holding from 12 to 18 carbons have an inhibitory effect.View full abstractDownload PDF (794K) -
Norimasa HIRANO, Kiyo SUSHIDA1966 Volume 21 Issue 9 Pages 552-556
Published: September 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSWe isolated 165 strains of mycobacteria from the sputum of tuberculous patients. Their biological characteristics and pathogenicity in guinea pigs were tested. The results were as follows.
1. Some mycobacteria were able to grow on the nutrient agar, but the others did not grow on that agar.
2. The biological characteristics of these isolated strains were almost identical with that of M. tuberculosis in respects to niacin test, neutral red test, catalase test and Kf test.
3. Both two kinds of strains, one could grow on nutrient agar and the other could not grow, caused difinite lesions in guinea pigs when these strain were inoculated subcutaneously into the animals. In some lesions caseous necrosis, epithelioid cells and giant cells were found, and it was difficult to distinguish these lesions from the true tuberculous lesions. From the above facts, we believe, that there is no doubt that acid-fast bacilli used in the tests and exhibiting properties not ordinarily associated with M. tuberculosis are nevertheless tubercle bacilli.
4. When the sputum of tuberculous patients, which was treated one or two hours with one per cent NaOH, was immediately planted on the nutrient agar, we found a very small transparent colony by hand lens. This colony had grown a little larger after successive transfers on the nutrient agar and the niacin test of this strain yielded a positive result; and this strain was pathogenic in guinea pigs.View full abstractDownload PDF (627K) -
Kiyoto AKAMA, Shoichi KAMEYAMA, Akiharu ITO, Ryosuke MURATA1966 Volume 21 Issue 9 Pages 557-563
Published: September 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSElek's method has been generally used to examine the toxinogenicity of Corynebacterium diphtheriae in vitro, but the results of tests were not always satisfactory. We succeeded to produce a peptone suitable for toxin production of C. diphtheriae. We also found that papain digests of sera promoted greatly the growth of the organisms on agar media in the absence of serum. These observations made us possible to prepare a new medium for Elek's test. This medium consists of: 1.2% digested sera, 2.5% peptone (papain digest of the whale meat), 0.2% yeast extract, 1.0% maltose, 0.1% NaCl and 1.5% agar. The pH is adjusted to 7.6 and the medium is sterilized for 20 minutes at 120°C. The medium can be stored in a powdered form. The elimination of the serum facilitated the preparation and sterilization of the medium. It was also demonstrated that the antitoxin could be stored in a dried state on the filter paper.View full abstractDownload PDF (2468K) -
Kiyoto AKAMA, Sho OTANI, Shoichi KAMEYAMA, Akiharu ITO, Ryosuke MURATA1966 Volume 21 Issue 9 Pages 564-573
Published: September 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSIn Japan, glucose blood agar plate has been widely used for the isolation of clostridia including Clostridium perfringens. Recently, egg yolk media with Neomycin sulphate were recommended for this purpose. However, the utility of such media is largely dependent on the basal medium as well as the accessory reagents added. We prepared a reproducible medium containing Kanamycin and studied the effects of various constituents of the medium on the isolation of Cl. perfringens. The basal medium consists of 0.5% heart infusion (paste), 1.0% peptone prepared from meat, 1.0% peptone prepared from milk casein, 0.5% NaCl, 1.0% lactose, 0.005% phenol red, 0.02% Kanamycin sulphate and 2.0% agar. The pH was adjusted to 7.6. This basal medium is added with 50% suspension of egg yolk to 10 per cent. Kanamycin should be omitted for the examinations of heated samples, because of the poor growth of the heat treated organisms on the media with Kanamycin.
The basal medium has the following advantages:
(a) It can be used for egg-yolk agar as well as blood agar, since the dye containing in it does not interfere with the developement of green colour of the colony of Cl. perfringens on the latter medium. (b) No difference was found in the ability to detect the organisms between blood agar and egg yolk agar, so long as this basal medium was used. The results were also comparable with those by cooked meat media. (c) This egg yolk medium may also be used as a medium for identification of Cl. perfringens when combined with the specific antitoxin.View full abstractDownload PDF (1006K) -
Kyosuke OZAWA, Tsuneo MEGURO, Kazuko TAKEUCHI1966 Volume 21 Issue 9 Pages 574-581
Published: September 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSI. In the incubational test on several human feces using human feces medium treated with SM., the following two cases are observed:
1) Yeast like bacteria are seen to increase extraordinary.
2) The increase of yeast like bacteria can not be seen, but numerous anaerobic bacilli, especially gram positive bacilli are detected.
On the other hand, even in case anaerobic bacilli are not detected, it can be recognized that the extraordinary increase of yeast like bacteria is remarkably checked by the administration of drug resistant Str. fecalis.
II. The crossing incubational test on two kinds of human feces different in anaerobic bacilli flora in mediums made from each kind of feces above shows that, in the medium made from feces including many anaerobic gram positive bacilli, the same bacilli can not increase, and the extraordinary increase of yeast like bacteria is observed.
According to the result, it can be inffered that anaerobic gram positive bacilli have cheking power on the increase of yeast like bacteria.View full abstractDownload PDF (677K) -
1966 Volume 21 Issue 9 Pages 582-605
Published: September 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (3761K)
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