Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
Search
Advanced search
OR
Browse
Search
Volume 42, Issue 2
Displaying 1-6 of 6 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • Reconstruction of the taxonomy of Genus Peptococcus and Peptostreptococcus and study on their identification-system
    Takayuki EZAKI
    1987 Volume 42 Issue 2 Pages 471-478
    Published: March 25, 1987
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.42.471
    JOURNAL FREE ACCESS
    Download PDF (727K)
  • Coagulase-negative staphylococci and Staphylococcus hyicus subsp. hyicus
    Akira SHIMIZU
    1987 Volume 42 Issue 2 Pages 479-497
    Published: March 25, 1987
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.42.479
    JOURNAL FREE ACCESS
    Download PDF (2129K)
  • A serogrouping scheme for Campylobacter jejuni by slide agglutination method with formalin-treated antigens
    Kahiko SAITO, Ichiro TAKANO, Masaki TAKAHASHI, Yoshitoki YANAGAWA, Tak ...
    1987 Volume 42 Issue 2 Pages 499-512
    Published: March 25, 1987
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.42.499
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    A serogrouping scheme for Campylobacter jejuni based on the slide agglutination technique with formalin-treated cells was developed.
    Cross-agglutination experiments between autoclaved (at 121C for 120min) cells prepared from six different strains of various souces and their antisera demonstrated no remarkable immunological specificity among the strains. While, the experiments using formalin-treated cells of various strains and their respective antisera revealed remarkable specificities among them, i.e., each of these antisera showed the highest agglutination titer against the homologous antigen, and cross reactions with heterologous antigens were weak, if any, even when unabsorbed antisera were examined. The cross reactions were removed by absorption. These results indicate that this organism has at least two kinds of antigenic factors, heat-stable and relatively heat-labile. The latter antigenic factor (s) responsible for agglutination in the system using formalin-treated antigen were not completly inactivated by heating at 100C for 60min.
    Thus, agglutination tests using formalin-treated antigens and their corresponding antisera were found to be a useful tool for serological grouping of this organism, and the slide agglutination technique was satisfactory for routine use. By this system, 33 serogroups, numbered from TCK 1-TCK33, have been identified.
    With these single grouping antisera, 1, 768 of 2, 305 or 77% of C. jejuni isolates from human gastroenteritis cases and 455 of 767 or 59% of those from animal sources were found typable; 6.3% of the human isolates and 3.7% of the animal isolates reacted with two or more grouping sera. The other 15% of the former and 37% of the latter were untypable, not reacting with any of the grouping sera. They are under further investigation.
    In a family outbreak, the serogroup of the isolates from patients coincided with that from the food incriminated epidemiologically as a common vehicle. In most of the community outbreaks studied, good serological correlation of the isolates was observed.
    The serogrouping system of C. jejuni by slide agglutination with formalin-treated cells is of great value in the studies of epidemiology of C. jejuni gastroenteritis and ecology of the organism.
    View full abstract
    Download PDF (1279K)
  • Studies on the Measurement of Antibody against C-Polysaccharide Antigen of Group A Streptococcus by the Enzyme-Linked Immunosorbent Assay
    Yuko TODOME, Hisashi OHKUNI, Kozo YOKOMURO, Shigeyuki HAMADA
    1987 Volume 42 Issue 2 Pages 513-516
    Published: March 25, 1987
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.42.513
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The present paper describes the measurement by ELISA of antibody against group A streptococcal specific C-polysaccharide in the immunized rabbit and human sera with trypsinpronase treated whole cells as antigen.
    The optimal concentration of the enzyme-treated whole cells for coating to wells was 2×107cells/well. Absorption tests with whole cells of group A streptococcus, purified C-polysaccharide and N-acetylglucosamine indicated that the antibody specific to C-polysaccharide was detected by ELISA. There was a highly significant correlation between the anti-C-polysaccharide antibody titrated with enzyme-treated whole cells and that with purified C-polysaccharide as antigen. When the antibody titers (IgG class) against the enzyme-treated whole cells of the sera of patients with acute post-streptococcal glomerulonephritis and rheumatic fever were compared with those of the sera of healthy individuals by ELISA, the former was significantly higher than the latter.
    The results suggest that anti-C-polysaccharide antibody can be measured by ELISA with enzyme-treated whole cells as antigen.
    View full abstract
    Download PDF (399K)
  • [title in Japanese]
    1987 Volume 42 Issue 2 Pages 517-524
    Published: March 25, 1987
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.42.517
    JOURNAL FREE ACCESS
    Download PDF (1022K)
  • [title in Japanese]
    1987 Volume 42 Issue 2 Pages 525-533
    Published: March 25, 1987
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.42.525
    JOURNAL FREE ACCESS
    Download PDF (1209K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top