Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 25, Issue 12
Displaying 1-3 of 3 articles from this issue
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  • Studies on Acinetobacter anitratus
    I. TB-MacConkey Agar Plate as a Screening Tool for A. anitratus
    Takamasa SHIMIZU, Kazuya UCHIMURA
    1970 Volume 25 Issue 12 Pages 641-644
    Published: December 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.641
    JOURNAL FREE ACCESS
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    The TB-MacConkey agar plate, prepared from commercial MacConkey agar medium supplemented with 0.4% tributyrin, was proved to be useful for detecting Acinetobacter anitratus from contaminated chick embryos. Almost all the colonies showing lipolysis on this plate were those of A. anitratus.
    When this selective medium was used for bacterial detection from other materials, such as soil and water, some Gram-negative bacilli, e.g., Pseudomonas, Alcaligenes, Flavobacterium and Aeromonas, also formed colonies surrounded with zones of opacity. They were easily discriminated from A. anitratus by a set of screening tests; namely, O-F test, oxidase test, properties on KI medium and motility test.
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  • Studies on Acinetobacter anitratus
    II. Contamination of Hatchery Environments with A. anitratus
    Takamasa SHIMIZU, Kazuya UCHIMURA
    1970 Volume 25 Issue 12 Pages 645-650
    Published: December 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.645
    JOURNAL FREE ACCESS
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    Acinetobacter anitratus was isolated predominantly, at a rate of 23 to 55%, from dead-in-shell chicks and weak chicks originated from three out of four separate commercial hatcheries. The frequency of isolation of this organism rose in accordance with the advance in embryonic development within the test material. This organism was isolated from none of 575 samples collected from the fourth hatchery tested in 8 surveys during two years.
    The laying hen, pig, dog and rabbit, as well as man, were proved not to be frequent carriers of A. anitratus.
    The organism was revealed to be distributed most commonly in the soil. Some of the samples collected from fresh water, fish, meat, and crabs were also contaminated with A. anitratus.
    The results of the test of the isolates for susceptibility to a virulent phage suggested that a specific type of A. anitratus might persist to contaminate a hatchery environment.
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  • [title in Japanese]
    1970 Volume 25 Issue 12 Pages 651-686
    Published: December 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.651
    JOURNAL FREE ACCESS
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