Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 46, Issue 5
Displaying 1-5 of 5 articles from this issue
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  • Biological and pharmacological properties of Clostridium perfringens α toxin
    Yoshio FUJII
    1991 Volume 46 Issue 5 Pages 817-826
    Published: September 25, 1991
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.46.817
    JOURNAL FREE ACCESS
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  • Studies on Fundamental Mechanisms in Host-Parasite Relationships with Special Reference to Etiology of Nontuberculous Mycobacteriosis
    Haruaki TOMIOKA, Hajime SAITO
    1991 Volume 46 Issue 5 Pages 827-837
    Published: September 25, 1991
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.46.827
    JOURNAL FREE ACCESS
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  • Antibacterial and bactericidal activities of tea extracts and catechins against methicillin resistant Staphylococcus aureus
    Masako TODA, Sachie OKUBO, Yukihiko HARA, Tadakatsu SHIMAMURA
    1991 Volume 46 Issue 5 Pages 839-845
    Published: September 25, 1991
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.46.839
    JOURNAL FREE ACCESS
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    We examined tea extract, (-) epigallocatechin gallate (EGCg) and theaflavin digallate (TF3) for their antibacterial and bactericidal activities against methicillin resistant Staphylococcus aureus (MRSA) and food poisoning strains of S. aureus. Twenty percent tea extract (50μl), EGCg (63μg) and TF3 (125μg) added to one ml of culture medium each inhibited the growth of all strains of MRSA and food poisoning S. aureus tested. Tea extract showed also a bactericidal activity against MRSA even at the same concentration of as in ordinarily brewed tea. EGCg at a concentration of 250μg/ml showed a bactericidal activity against MRSA but not against food poisoning S. aureus, but at 500μg/ml reduced markedly the viable number within 48h. These results suggest that tea and catechin can be used as prophylactic agents against MRSA infection.
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  • Augmentation of activities of peripheral granulocytes and of resistance against bacterial infection after administration of G-CSF into mice
    Nariyoshi SHINOMIYA, Sumiaki TSURU, Yoshiya KATSURA, Masayuki NORITAKE ...
    1991 Volume 46 Issue 5 Pages 847-854
    Published: September 25, 1991
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.46.847
    JOURNAL FREE ACCESS
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    We evaluated the metabolic capability of murine peripheral granulocytes after administration of recombinant human granulocyte colony-stimulating factor (rhG-CSF) by quantitative flow cytometric assay for H2O2-dependent oxidative product formation. Intraperitoneal administration of a daily dose of 10μg of rhG-CSF for 5 days induced doubling of the leukocyte population. Differential counting of peripheral leukocytes and scattergram by flow cytometry showed an increased mature granulocyte population. After stimulation with phorbol myristate acetate, the granulocytes of the rhG-CSF-administered mice demonstrated some hyperresponsive population and an increased H2O2 production. The hyperresponsive population showed H2O2 production 4-6 times higher than did normal cells. Granulocytes from the G-CSF-treated mice revealed an augmented phagocytic activity and an increased expression of Mac-1 molecules. Moreover, mice treated with G-CSF showed an enhanced resistance against intravenous infection with a lethal dose of E. coli. Granulocytes showing such markedly increased oxidative metabolism may be a significant component of the host defence to various infective organisms.
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  • Cross reactive antigens of Acholeplasma laidlawii and L-form of Staphylococcus aureus
    Takanori KAWASHIMA, Koichi KUWANO, Ikuko MATHU-URA, Satoshi FUKUSE, Su ...
    1991 Volume 46 Issue 5 Pages 855-860
    Published: September 25, 1991
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.46.855
    JOURNAL FREE ACCESS
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    We demonstrated that the membrane of Acholeplasma laidlawii PG8 and L-form of Staphylococcus aureus, both of which induce cellular immunity in BALB/c mice, were antigenically related each other. Foodpad responses of the mice immunized with a mixture of either antigen and Freund's complete adjuvant showed clearly a cross reaction when challenged with the other antigen. Cross responses to incorporate 3H-thymidine to the spleen lymphocytes of the mice immunized with either antigen occured in the presence of the other antigen.
    Furthermore, the purified T cells, but not B cells, of the spleen were activated in the presence of antigen-presenting cells. These antigens existing in the membrane fractions of both microorganisms were purified by Razin's method. Finally, these membrane components of A. laidlawii and L-form of S. aureus were subjected to gel electrophoresis and transferring to nitrocellulose membrane and used to stimulate the spleen lymphocytes of the mice immunized with A. laidlawii or of non-immunized mice. The fractions representing molecular weights of approximatly 45kD, 25kD, and 13kD of both microorganisms consistently stimulated the lymphocytes of the immunized mice but not those of non-immunized mice.
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