Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
Search
Advanced search
OR
Browse
Search
Volume 25, Issue 4
Displaying 1-6 of 6 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • Studies on the Bacterial Spore Coat
    1) Morphological Structure of the Spore Coat of Bacillus megaterium
    Chikataro KAWASAKI, Tsutomu NISHIHARA, Masaomi KONDO
    1970 Volume 25 Issue 4 Pages 209-214
    Published: April 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.209
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The spore coat structures of two strains of Bacillus megaterium were studied. The coat residues isolated from the dormant spores of both strains were fractionated successively by alkali-treatment, sonication, and enzymatic digestion. Then morphological changes of the residues were observed electron-microscopically at each step of the treatment. From the observation, it was suggested that the spore coats of both strains might consist of two layers, and that the inner layer might be composed of a fibrillar skeleton structure containing alkali-soluble material as a structural component between the two layers.
    View full abstract
    Download PDF (3863K)
  • Studies on the Bacterial Spore Coat
    2) Chemical Structures of Fractions Isolated from the Dormant Spore Coat of Bacillus megaterium
    Masaomi KONDO, Tsutomu NISHIHARA
    1970 Volume 25 Issue 4 Pages 215-221
    Published: April 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.215
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The chemical structures of three characteristic fractions each isolated from the dormant spore coats of Bacillus megaterium QM B1551 and B. megaterium ATCC 19213 were studied. The paracrystal fractions corresponding to the fibrillar structure of the inner coat composed mainly of keratin-like substance and alkali-soluble fractions consisted of acidic protein (isoelectric point: pH 4) which mainly contained aspartic acid and glutamic acid. No remarkable differences were seen chemically or physically between those fractions isolated from both strains. The resistant fraction corresponding to the outer spore coat of B. megaterium QM B1551 was composed mainly of phosphomucopeptide, but the fraction isolated from B. megaterium ATCC 19213 had a different chemical structure in phosphorus and hexosamine contents.
    View full abstract
    Download PDF (733K)
  • Selective Isolation Media for Strictly Anaerobic, Non-Sporulating Gram-Negative Rods
    Fumishige OHTANI
    1970 Volume 25 Issue 4 Pages 222-232
    Published: April 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.222
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Many media have been developed for the isolation and cultivation of strictly anaerobic, non-sporulating Gram-negative rods. In this study, some of them were modified and improved for the selective growth of the genera Bacteroides, Sphaerophorus and Fusobacterium, using a number of freshly isolated and stock strains.
    The following media were proved to be the most satisfactory for this purpose.
    1) As a selective cultivation medium for Bacteroides: CNBGT (colistin-neomycin-brilliant greenglucose-taurocholate) medium which was derived from Mitsuoka's medium.
    2) As a selective cultivation medium for Sphaerophorus and Fusobacterium: Modified FM medium (containing pepsin-digested cow's blood and neomycin) which was derived from FM medium (Difco; containing sheep blood and streptomycin).
    View full abstract
    Download PDF (1120K)
  • [title in Japanese]
    [in Japanese]
    1970 Volume 25 Issue 4 Pages 233-238
    Published: April 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.233
    JOURNAL FREE ACCESS
    Download PDF (873K)
  • [title in Japanese]
    1970 Volume 25 Issue 4 Pages 239-258
    Published: April 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.239
    JOURNAL FREE ACCESS
    Download PDF (3016K)
  • [title in Japanese]
    1970 Volume 25 Issue 4 Pages 259-267
    Published: April 25, 1970
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.25.259
    JOURNAL FREE ACCESS
    Download PDF (1261K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top