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Volume 21, Issue 10
Displaying 1-7 of 7 articles from this issue
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Momoe SOEDA, Michiko OTOMO, Mieko OME, Kazuko KAWASHIMA1966 Volume 21 Issue 10 Pages 609-614
Published: October 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSCape aloe is a dried product of juice of Aloe ferox Miller or of the cross-bred between Aloe ferox Miller and Aloe africana Miller, usually brownish black to dark brown in color. When it is heated to evaporize and crushed after cooling, it becomes to dark red to brownish yellow powder which is called Aloe powder in the Japanese Pharmacopoeia.
On the other hand, Aloe juice producee by Mamiya Pharmaceutical Co. is an yellow liquid obtained from original plants by means of repeated freezing and thawing. When it is passed through Seitz filter and then freeze-dried, it becomes to fine yellowish powder which we call freeze-dried Aloe powcer.
Cape aloe or Aloe powder has been used as a peptic or a laxative in medicine, and Aloe juice is now widely used as a medicine for gastro-intestinal disturbances or as an ointment for burns or frostbites in popular remedies.
In an attempt to clarify the basic mechanism by which Aloe Juice exerts its beneficial effect on the skin lesions such as burns, frostbites and insectbites, its anti-bacterial and antifungal activity against representative 16 strains and its therapeutic effect on experimental trichophytiasis produced on the back skin of guinea pigs as well as on 12 human cases with trichophytiasis have been tested. Although the bactericidal and fungicidal activity of Aloe juice is not so noteworthy, it exerts a cidal action against a wide variety of bacteria and fungi, particularly against Pseudomonas aeruginosa and Proteus vulgaris.
Aloe ointment containing 5% Aloe freeze-dried powder proved to be considerably effective in treatment of experimental or human trichophytiasis, when applied once a day for about 10 days. The test on therapeutic effect of Aloe juice on burns experimentally infected with Pseudomonas aeruginosa is now in progress, which will be reported on in the near future.View full abstractDownload PDF (743K) -
III. Mycobacterium marinumMichio TSUKAMURA, Shoji MIZUNO, Haruo TOYAMA, Sumio TSUKAMURA1966 Volume 21 Issue 10 Pages 615-618
Published: October 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSCharacters of Mycobacterium marinum, based on tests of six strains, are as follows:
Colonial Morphology: Usually rough, but it may be smooth. Colonial pigmentation: Negative, when cultivated without light. Some strains are photochromogenic. Nitrate reduced. Two-week-arylsulfatase activity: Positive. Urease: Positive. Allantoinase: Usually positive. Other amidases of Bönicke's ten amidases, acetamidase, benzamidase, isonicotinamidase, nicotinamidase, pyrazinamidase, salicylamidase, succinamidase and malonamidase: Negative. Catalase: Positive. Peroxidase: Negative. Niacin: Negative.
Growth on 0.5mglml and 1.0mg/ml sodium salicylate-Ogawa egg medium: Positive. Growth on 0.2% PAS-Ogawa egg medium: Positive. Growth on 0.25mg/ml. NH2OH-HCl-Ogawa egg medium: Positive. Growth on 0.25mg/ml 8-azaguanine-Ogawa egg medium: Positive.
Growth rate: Slow; growth at 7 days on egg media; growth at 7 to 14 days on Sauton agar.
Growth at 28°C and 37°C, but no growth at 45°C.
Utilization of carbohydrates as sole carbon source: Acetate, citrate, succinate, malate, pyruvate, benzoate, malonate and fumarate not utilized; glucose, fructose, sucrose, ethanol, propanol, propylene glycol, 1, 3-, 1, 4- and 2, 3- butylene glycols not utilized; mannose, galactose, arabinose, xylose, rhamnose, trehalose, raffinose, inositol, mannitol and sorbitol not utilized in the presence of ammoniacal nitrogen. No acid from glucose, mannose, galactose, arabinose, xylose, rhamose, trehalose, raffinose, inositol and sorbitol.
Utilization of nitrogen compounds as sole, simultaneous carbon and nitrogen source; L-Glutamate utilized usually; L-serine, glucsamine-HCl, acetamide, benzamide, monoethanolamine and trimethylene diamine not utilized.
Utilization of nitrogen compounds as sole nitrogen source: L-Glutamate, urea, succinamide and nitrate utilized; L-serine, pyrazinamide and nicotinamide utilized by three of six strains; L-methionine, acetamide, benzamide, isonicotinamide and nitrite not utilized.
Two types of M. marinum were differentiated according to the pattern of utilization of carbohydrates, in the presence of glutamate-nitrogen or trimethylene diamine-nitrogen. The first biotype utilized (showed growth) glucose, acetate and pyruvate in the presence of glutamate-nitrogen and utilized acetate and pyruvate in the presence of trimethylene diaminenitrogen. To this type, three strains received as M. marinum and M. balnei belonged. Another biotype utilized none of glucose, fructose, sucrose, acetate, citrate, succinate, malate, pyruvate, malonate and fumarate in the presence of one of the above nitrogen sources. Three strains received as M. ranae, M. piscium and M. platypoecilus belonged to the latter type.View full abstractDownload PDF (384K) -
I. Counts of the Organisms in Unheated and Heated Fecal SpecimensKiyoto AKAMA, Sho OTANI, Shoichi KAMEYAMA, Akiharu ITO, Ryosuke MURATA1966 Volume 21 Issue 10 Pages 619-625
Published: October 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe distribution of Clostridium perfringens in the normal human intestinal tracts was investigated in order to provide the proper interpretation of the results of bacteriological examinations to be undertaken when a food poisoning breaks out. Cl. perfringens was detected in almost all the fecal specimens from healthy persons. The actual count ranged from 101 to 108 per one gram of feces. The total count of the organisms detected in both heated and unheated feces fluctuated considerably, even in specimens from the same person. The fluctuation of the count fell within a certain range, although the counts often varied depending upon each individual.
The counts of the organisms detected in the specimens heated at 80C correlated to a certain extent to those in the corresponding unheated specimens, but such a correlation was not found when the specimens were heated at 90C or higher temperature. Such organisms that resisted against the heating at 100C for 60 minutes were detected in the specimens from healthy persons at about 50 per cent, and the count ranged from 101 to 106 per one gram of the feces.
The organisms lost the heat resistance when grown in a medium containing carbohydrate. General failure encountered in isolating Cl. perfringens from the foodstuffs responsible for outbreaks of food poisoning may be explainable from the action of carbohydrate included in the foodstuffs.View full abstractDownload PDF (730K) -
Yoko SASAGAWA, Shiyohei FUJINUMA, Mozo SAGAWA1966 Volume 21 Issue 10 Pages 626-628
Published: October 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe titration of diphtheria antitoxin was carried out a method using collodion paticles sensitized with diphtheria toxin. The results coincides well with these obtained by the hemagglutination.
In addition, this titration was 10 to 100 times more sensitive than the intracutaneous reaction.View full abstractDownload PDF (283K) -
Masahiro KONDO, Kanji TSUCHIYA1966 Volume 21 Issue 10 Pages 629-632
Published: October 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe influence of 5-n-buthyl-1-cyclohexyl-2, 4, 6-trioxoperhydropyrimidine (BCP) on hemolysin formation against sheep red cells in mice was observed in comparison with that of cortisone. The drug was administered subcutaneously once daily for 4 days, starting from the 3rd day before (I), at the same time with (II) and on the 3rd day after (III) the intraperitoneal sensitization. While BCP gave no influence upon the antibody formation in (I), (II) and (III), cortisone suppressed markedly the hemolysin titer in (I) and (II), but showed no influence in (III).View full abstractDownload PDF (364K) -
Studies on the Tribe Neisserieae in the Family Coccaceae and Isolation and Certification of Species in a New Genus Halococcus Induced TheoreticallyKohtaku HAYASHI, Tomiko KODAIRA, Kumiko BABA, Kazuko KIKUCHI1966 Volume 21 Issue 10 Pages 633-639
Published: October 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSStudy on the classification and reorientation for the classical family Neisseriaceae, being based on the Adanson's thinking, Sneath's similarity value and the author Hayashi's concept of center species was made and the following results were obtained.
1) The classical family Neisseriaceae should be subordinated tribe Neisserieae which consists of three genera Neisseria, Veillonella and Halococcus.
2) A new genus Halococcus was induced theoretically in accordance with the relationship and classification of the whole cocci group.
3) The correlated features of genus Halococcus were as follows: Gram negative, pair, aerobic, halophilic and do not ferment glucose.
4) From the general consideration of cocci group according to the above method and the concepts, the author proposed that cocci group should be integrated as one family Coccaceae which includes four tribes, Micrococceae, Sarcineae, Streptococceae and Neisserieae.
5) The classification of family Coccaceae and the key features of each tribes and genera, presented by the authors, are as follows.View full abstractDownload PDF (649K) -
1966 Volume 21 Issue 10 Pages 640-644
Published: October 25, 1966
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (607K)
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