1) From the heated cell extract of B. anthracis, an antigenic substance was isolated, through trichloracetic acid treatment, the removal of lipids by ether, aceton fractionation and zone electrophoresis, which was suggested to be homogeneous by the analytical centrifugation and agar diffusion test. 2) This substance reacts with anti-anthracis serum in 32000-fold dilution, but not with anti cereus and anti-subtilis serum; it is specific in the above sense. 3) This substance was positive in Biuret reaction and Folin reaction and showed the absorbtion of maximum in 280 mu, while negative in sugar and nucleic acid reaction. These facts strongly suggest this substance to be protein like.
The biological characteristics of two kinds of TC-resistant strains, one of which was obtained by transduction with phage 81, using as a donor strain the tetracyclin-resistant S. aureus H-37, and as recipient the tetracyclin-sensitive S. aureus H-56 and H-64, and the other by the step-by-step method, were compared. Betweela the original strains and transductants, there were no differences in the production of coagulase and alphalysin, the contents of beta-carotin, phosphatase activity, toxicity to mice and the growth curve. The tetracyclinresistant strains of H-56 and H-64 obtained by the step-by-step method, however, showed the marked decrease in the production of coagulase, and alpha-lysin and in the toxicity to mice, and no trace of bata-carotin and phosphatase and more delayed growth curve.
In the previous work, the authors reported on the clinical and pathological findings in four monkies introduced with dysentery becilli into the stomach and the intestinal lumen. This report describes on the histopathological evaluations of these animals. Ml was operated upon, and bacilli were injected directly into the proximal duodenum; M 4 into the proximal caecum. They produced lesions similar to those seen in human acute small intestinal dysentery. M 2 was administered directly into the stomach. The animal revealed the lesions developed especially in the large intestine, showing acute pseudomembranous enteritis. In these animals, various degenerative changes were presented in other organs including stomach, intestinal tract, heart, lung, kidney, brain, liver and spleen. Degenerative changes in the brain were dilatation of Robin's space, degeneration of pyramidal cells, pyknosis of glianucleus, pseudoneuronphagy and karyoklasis of Purkinje's cells. In the liver, degenerative atrophia of nucleus and diffuse fatty droplets around sinusoids in the liver cells were observed. In the spleen, slight hyaline degeneration of central artery was observed.
For the purpose of differentiating various kinds of acid-fast bacilli, especially M. tuberculosis var. hominis and atypical mycobacteria, we performed studies on their respiration using Warburg's manometer. The acid-fast bacilli we examined are: M. tuberculosis var. hominis (three strains), BCG, M. avium (strain Kirchberg), non-pathogenic mycobacteria (five strains), atypical mycobacteria (five strains) isolated by us, and stypical mycobacteria originated in U. S. A. Glycerine, lactic acid, succinic acid, pyruvic acid and dodecan are added as a substrate in manometer and oxygen absorption rate of each group are observed. The oxygen absorption rate of atypical mycobacteria showed remarkable increase, only when dodecan is added. The rate of M. tuberculosis var. hominis and non-pathogenic mycobacteria, on the contrary, showed rather lower levels than their self-respiration rate. On the cases of BCG and Kirchberg's strain, the rate increased not only with dodecan, but also with glycerine and salicylic acid. As the results of these findings, we can conclude that M. tuberculosis var. hominis, atypical mycobacteria, and non-pathogenic mycobacteria might be easily differentiated from the aspects of respiratory metabolism, if we compare their respiration rate under dodecan, glycerine and salicylic acid.