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Volume 26, Issue 10
Displaying 1-6 of 6 articles from this issue
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Observation on Chronic Otitis Media Infected with the PseudomonasAkiko TAKAI, Shiro NAGAO, Hiromu MORI1971 Volume 26 Issue 10 Pages 467-472
Published: October 25, 1971
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe authors made an attempt to determine whether or not humoral antibodies were present in patients of chronic otitis media with otorrhea which was positive for the presence of Pseudomonas aeruginosa. Sera from thirty-five patients were examined by Boyden's method for the hemagglutination reaction of tanned sheep red cells coated with an aqueous extract of the bacterial cells. It was common that these sera showed a positive reaction at a high titer (1/40 to 1/640; or higher titers being reached in some cases) when tested against the bacterial antigen of homologous origin or against that from their own otorrhea. Whereas, heterologous matchings usually presented much lower titers. Sera from healthy persons had inevitably very low titers (≤1/20) commonly against different antigens.
Then, the authors applied the present technique to sera from rabbits given intravenous injection with a heated suspension of the bacteria. Nine strains classified into different serological types by Homma's method were selected for the study designed. No appreciable antigenic overlapping was proved among these strains when examined by the cross absorption test and the cross inhibition test by the use of the present hemagglutination method.
These findings suggested that antigen or antigens which seemed to be specific for individual strains of P. aeruginosa might be responsible for the present reaction.View full abstractDownload PDF (692K) -
Toshizo SAKURAI, Kiyoshi TOHYAMA, Hiroshi ARAI1971 Volume 26 Issue 10 Pages 473-481
Published: October 25, 1971
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA phage, PLS-1, derived from Lactobacillus salivarius examined for several properties. Treatment with mitomycin C at a concentration higher than 2mcg/ml or ultraviolet (UV) irradiation using a 15-watt ultraviolet lamp at a distance of 90cm for 30 to 60 seconds (5.4ergs/cm2/sec) was proved to be required for the efficient induction of phage PLS-1 from its original lysogenic strain Sa-S. The “cured” strains derived from strain Sa-S were shown to be effective as indicators for phage PLS-1. Phage PLS-1 had a latent period of approximately 80 minutes and its burst size ranged from 100 to 130. Comparison was made on sensitivity to UV irradiation and serological specificity between phage PLS-1 and some other Lactobacillus phage.View full abstractDownload PDF (1955K) -
Kiyoshi TOHYAMA, Toshizo SAKURAI, Hiroshi ARAI1971 Volume 26 Issue 10 Pages 482-487
Published: October 25, 1971
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA temperate phage designated as PLS-1 was studied on transducibility and lysogenization. It had been derived from the lysogenic SaS strain of Lactobacillus salivarius. This paper deals for the first time with the transduction in Lactobacilli.
Survival or lysogenized fractions were affected remarkably by phage-adsorption medium and temperature. For instance, the survival fractions of PLS-1-adsorbed cells in International Lactobacillus Subcommittee (ILS) medium at 37C were 7, 9, and 20% at a multiplicity of infection 10, 5, and 1, respectively. These fractions increased to 33, 41, and 67% in correspondence with the multiplicity of infection mentioned above, by changing the adsorption temperature to 30C and the adsorption medium ILS to yeast peptone broth. This change resulted in an increase in fractions of lysogenized colonies in survivors from 25 to 50% at a multiplicity of infection 10. In any of the conditions examined, nonlysogenic and phage-PLS-1-sensitive colonies appeared in the survivors at a high frequency.
This phage carried out a generalized transduction in L. salivarius. Of all the auxotrophic mutants, those requiring lysine, prolin, or serine were transduced to prototrophy at a frequency of 10-7 to 10-8. A lactose-non-fermenting mutant was also transduced to a fermentable wild type at a similar frequency. Most of the transductants were proved to be sensitive to phage PLS-1 when a low multiplicity of infection (less than 2) had been applied to them.View full abstractDownload PDF (689K) -
Kaname SUZUKI, Seigo ISOGAI, Hajime HASHIMOTO1971 Volume 26 Issue 10 Pages 488-492
Published: October 25, 1971
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSEpidemiological investigations were performed using 214 strains of Escherichia coli from 114 pigs, which had been chosen randomly on 114 farms having more than 100 pigs. The results obtained are summarized as follows.
1. Of the 114 pigs, 96 (84%) had SM-, 91 (80%) TC-, 68 (60%) SA-, 23 (19%) KM-, 19 (17%) APC-, and 3 (3%) CM-resistant bacteria.
2. TC, SM, SA (25%) and TC, SM (24%) were the most frequent patterns of drug resistance.
3. Of 214 resistant bacteria isolated, 69 (32%) were possesed R factors; that is, their drug resistance could be transferred by mixed cultivation.
4. The incidence of transferable drug resistance was higher in strains with APC resistance than in those without it.View full abstractDownload PDF (483K) -
II. Pathogenicity for Mice and RabbitsMasahiro MURAKAMI1971 Volume 26 Issue 10 Pages 493-498
Published: October 25, 1971
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSEight freshly isolated strains of Staphylococcus epidermidis were examined for pathogenicity for mice and rabbits. Of them, four isolates had been obtained from the blood of patients suffering from chronic septicemia and the other four from purulent lesions. No strains exerted any lethal effect upon mice when inoculated by the intravenous route. Some of the inoculated mice were revented from gaining in body weight. The organism was proved to be able to propagate in the kidney and the bone marrow. It also produced suppurative lesions in mice when inoculated subcutaneously. When inoculated intracutaneously into rabbits, all the strains produced inflammatory lesions, without inducing any abscess formation.
These results suggest that all the virulent strains of S. epidermidis studied may have an ability to cause chronic infection in man.View full abstractDownload PDF (790K) -
Hideo KINEBUCHI1971 Volume 26 Issue 10 Pages 499-502
Published: October 25, 1971
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (3095K)
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