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Volume 19, Issue 7
Displaying 1-3 of 3 articles from this issue
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1. Lysogenization of Myc. sp. jucho by the phages Y 13-L and -SYasuo MIZUGUCHI1964 Volume 19 Issue 7 Pages 169-174
Published: July 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSLysogenization rates of Mycobacteriaby mycobacteriophageswere investigated using the systems of the rapidly growing Mycobacterium, strain Jucho, and the two phages, Y 13-L and-S. Changes of bacterial characters accompanied with the lysogenization, especially phage susceptibilities and sugar availabilities, were also studied.
The lysogenization rate by Y 13 S was high, 27 %, and that by Y 13 L was rather low, 4.3 %. It was shown that the phage spontaneously released from the bacterium previously lysogenized by Y 13 L, was more temperate than the original with a higher lysogenization rate of 31-47 %.
The rate of spontaneous phage release of Myc. jucho (Y 13 L) was 3.5 ×10-1 and that of Myc. jucho (Y 13 S) was 1.1×10-3.
The host range was studied comparatively between the orginal Y 13 L phage and its more temperate one described above, against 119 strains of rapidly growing Mycobacteria, using the RTD spot technique, and only slight differences were observed.
Following changes in the phage susceptibilities of the lysogenized Mycobacteria from that of the original one were observed: Myc. jucho (Y 13 L) was resistant to the phage A 6 and D 4, and Myc. jucho (Y 13 S) was partially resistant to the phage HC, though the original strain was sensitive to those phages. Neutralization test by anti-phage sera has shown the closed relation among Y 13 L, A 6 and D 4 and between Y 13 S and HC, respectively.
Acid production patterns from carbohydrates of the lysogenized mycobacteria showed no differnce from those of the original.
Attempts of the phage induction of the lysogenized bacteria employing UV irradiation were carried out with no success.View full abstractDownload PDF (983K) -
Kohtaku HAYASHI1964 Volume 19 Issue 7 Pages 175-180
Published: July 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSClassification of microorganisms should be based on the cross-relationship of related species.
In this point of view, similarity value and a new concept “center species” are considered to be necessary for an ideal classification.
“Center species” are defined as follows: a species which has the most typical morphological and biological characteristics, thus it has the highest similarity value to other species being located in the center of a group of related species.
“Center species” is calculated from the reciprocal mean similarity value or the reciprocal mean dissimilarity value.
The use of a “center species” and the conditions for selecting a “center species” from a group of related species are discussed.
Provisional taxonomic ranks calculated from the similarity value and “center species” are proposed by the author.View full abstractDownload PDF (1086K) -
Kohtaku HAYASHI, Miyako KIMURA, Junko TANGO, Hiroko SANOGAWA, Nobuko S ...1964 Volume 19 Issue 7 Pages 181-187
Published: July 25, 1964
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSProteus, Morganella, Rettgerella and Providencia act on dl-phenyl alanine and produce phenyl pyruvic acid (PPA). From culture filtrate of above microorganisms, crystalline phenyl pyruvic acid was detected.
The authors intended to utilize this PPA production of microorganisms above described for the differentiation of the family Enterobacteriaceae and investigated into the composition of a new medium.
From results obtained, a new confirmatory medium which can also serve as differential medium was dev iced.
By the use of this medium it is possible to observe PPA-reaction, acid and gas production by fermentation of glucose or lactose and hydrogen sulphide production simultaneously. Proteus, Morganella, Rettgerella and Providencia produce a dark red-purple color on the slant surface of this medium.
Hence, the differentiation of Salmonella and Shigella from Proteus, Morganella, Rettgerella or Providencia which was impossible on Kligler's medium became possible.
Use of this new medium alone, therefore, will enable confirmation and differentiation of the family Enterobacteriaceae and it is considered useful as a routine clinical test.View full abstractDownload PDF (1134K)
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