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Volume 27 (1972)
- Issue 6 Pages 679-
- Issue 5 Pages 621-
- Issue 4 Pages 563-
- Issue 3 Pages 411-
- Issue 2 Pages 191-
- Issue 1 Pages 5-
Volume 27, Issue 6
Displaying 1-9 of 9 articles from this issue
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Takeshi YOKOTA1972 Volume 27 Issue 6 Pages 679-695
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
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Yoetsu HACHISUKA1972 Volume 27 Issue 6 Pages 697-708
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
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1972 Volume 27 Issue 6 Pages 709-735
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
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1972 Volume 27 Issue 6 Pages 737-790
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
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II. Isolation of an Active Compound Catalyzing the Synthesis of Dipicolinic Acid from the Diketopimelate-NH3 ComplexJun SAKURAI, Junko MIKI, Takao KONISHI, Masaomi KONDO1972 Volume 27 Issue 6 Pages 791-794
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSAn active fraction catalyzing the synthesis of dipicolinic acid from the diketopimelate-NH3 complex was extracted from sporangium. Its chemical properties were studied. To obtain the active compound, the cell-free extract of sporulating cells was fractionated by charcoal celite column chromatography and gel filtration on a column of Sephadex G-15 and G-25. The active fraction was subjected to preparative acrylamide gel electrophoresis. The active compound isolated was characterized by its partial resemblance to a related compound of peptidoglycan consisting of muramic acid, glycine, and phosphorus.View full abstractDownload PDF (426K) -
II. Growth and Morphological Characteristics in Liquid MediumTohru EDA, Shunsuke MATSUOKA, Ichiro TADOKORO1972 Volume 27 Issue 6 Pages 795-800
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSAn attempt was made to grow staphylococcal L-forms of strain STA-EMT-1 which had been produced at the authors' laboratory from a Staphylococcus aureus strain in a liquid culture medium. It was successful to grow L-form organisms collected from a solid medium in such liquid medium as consisting of brain heart infusion, 5% NaCl, and 10% horse serum. The L-form organisms were subcultured successfully in the liquid medium. It was demonstrated that the L-forms produced coagulase in the liquid medium. Growth in the liquid medium was slow initially, but after several transfers in the liquid medium the growth was found to be more rapid, reaching its maximum in 48 hours.
Macroscopically, the growth in the liquid medium appeared as a slime-like material sedimented at the bottom of the tube, but could be dispensed homogeneously by vigorous shaking. Microscopically, the liquid culture consisted of large spherical cells ranging from several to more than ten microns in diameter, fine granular elements, and amorphous substance. The minimal size of growth unit was less than 0.45 microns, as measured by the membrane filtration.
The staphylococcal L-forms seemed to be a stable type. Until the present time it has not reverted to the parent bacterial form during about thirty transfers in the liquid medium containing no penicillin. The staphylococcal L-forms presented the same sensitivities to various antibiotics as the parent strain, except that it showed a resistance to antibiotics which inhibit bacterial cell wall synthesis.View full abstractDownload PDF (4769K) -
III. Effects of Horse Serum, Sodium Chloride and Sucrose on Growth of Staphylococcal L-formsTohru EDA, Shunsuke MATSUOKA, Ichiro TADOKORO1972 Volume 27 Issue 6 Pages 801-807
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSHorse serum, sodium chloride, and sucrose were investigated for effect on growth and colonial characteristics of staphylococcal L-forms, strain STA-EMT-1 produced at the authors' laboratory, in comparison with those of Escherichia coli L-forms.
1. The best growth of staphylococcal L-forms was obtained on a solid medium containing more than 5% horse serum. With a decrease in the concentration of horse serum, their growth turned to be worse, although slight growth was still noticed even on a medium containing no horse serum at all.
Colony forms grown on solid media containing different concentrations of horse serum were as follows: Colonies assumed a typical “fried-egg” configuration on a 10% horse serum medium. Each colony had a rather small dark central area with an indefinite border on a 3% horse serum medium. Colonies had no central dark area, and consisted of an irregular-shaped material on a 1% horse serum medium.
2. No growth of staphylococcal L-forms was observed in a medium with sucrose added as a stabilizer of osmotic pressure, while E. coli L-forms could grow in this medium.
3. The best growth of staphylococcal L-forms was obtained by addition of NaCl at a concentration more than 3%. With a decrease in NaCl concentration, their growth diminished. No growth was observed at a concentration of NaCl less than 1%. On a medium containing 5% NaCl colonies showed a typical configuration: On a medium containing 2% NaCl the central area became smaller in each colony.
From the results mentioned above, it is recommended for the isolation of staphylococcal L-forms to use a medium consisting of brain heart infusion, 10% horse serum, 5% NaCl, and 1% special agar.View full abstractDownload PDF (4867K) -
Takashi SUNAGA, Taketoshi ARAI1972 Volume 27 Issue 6 Pages 809-815
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA simple and reliable assay method was devised for determination of the phenol oxygenase activity of Pseudomonas aeruginosa. One loopful of an overnight nutrient agar culture of a test organism was inoculated into 1ml of an assay medium consisting of 0.2% KH2PO4, 0.01% MgSO4⋅7H2O, 0.5% NaCl, 0.05% (NH4)2SO4, and an appropriate concentration of phenol compounds (final pH 7.0). In the case of protocatechuic acid (PA, 0.05%), protocatechualdehyde (PAl, 0.01%), salicylic acid (SA, 0.005%), gentisic acid (Ge, 0.08%), or catechol (Ct, 0.005%), 1ml of 1% FeCl3 solution was dropped into a 24-48 hours' culture of test microbes in the assay medium. A light yellow color appeared as a result positive for phenol oxygenase. When the test compound remained unchanged, a specific colonization took place to exhibit green-purple-black immediately after the addition. In the case of gallic acid (Gl, 0.0025%), pyrogallol (Py, 0.0025%), resorcinol (Re, 0.001%), phloroglucinol (Phl, 0.001%), phenol (Phe, 0.0025%), or o-cresol (Cr, 0.00025%), one or two drops of 2% phosphomolybdic acid solution and 2 to 4 drops of 10% aqueous ammonia were placed into the assay culture. A colorless reaction appeared as a result positive for phenol oxygenase. When the test compound remained unchanged, a blue colorization took place.
P. aeruginosa degraded Ge, PA, PAl, and Ct after incubation at 37C for 24-72 hours. P. fluorescens and other pseudomonads degraded PA, PAl, and Ct at 25C for 24 hours, but not at 37C for 72 hours. Ge was degraded by P. aeruginosa, but not by any other pseudomonads at 25C or 37C. Therefore, the degradation of Ge was highly specific to P. aeruginosa.
All the tested strains of Enterobacter B and Klebsiella degraded PA and PAl. Most of the Enterobacter B strains and about 50% of the Klebsiella strains degraded Ct. Most of the Enterobcter B strains and three of the Klebsiella strains degraded Ge after incubation at 37C for 72 hours. The other Gram-negative rods tested, however, failed to degrade any of the phenol compounds.View full abstractDownload PDF (720K) -
1972 Volume 27 Issue 6 Pages 821-844
Published: November 25, 1972
Released on J-STAGE: February 19, 2009
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