Nippon Saikingaku Zasshi

Transposable Element

Masanosuke YOSHIKAWA

1982 Volume 37 Issue 6 Pages 895-910
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.895

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Asakawa Prize and Dr. Asakawa

Tsunesaburo FUJINO

1982 Volume 37 Issue 6 Pages 911-914
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.911

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I love the microbiology more and more

Motoki YAMANAKA

1982 Volume 37 Issue 6 Pages 915-918
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.915

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On my research history

Keizo NAKAMURA

1982 Volume 37 Issue 6 Pages 919-922
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.919

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Adsorbing activity to guinea pig erythrocytes and mouse virulence of the K-9 strain of Klebsiella pneumoniae and its variants possessing different cell surface structure

Masami TAKAHASHI, Yukio OHSHIMA, Yukio USUI, Chizuko YOSHII, Tomohide ...

1982 Volume 37 Issue 6 Pages 923-930
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.923

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Mutant A' was newly isolated from strain K-9 of Klebsiella pneumoniae in addition to variants A, B and C reported previously, and subjected to examinations. Colonies of mutant A' were large and round with streaming tails in soft agar medium and large and nonmucoid on MacConkey agar plates. Small capsule-like structure was demonstrated with this mutant by negative staining under a light microscope. Such surface structure was found by electronmicroscopy being consisting of pili. Adsorption to guinea-pig erythrocytes and virulence to the mouse were compared among mutant A', the parent strain possessing large capsule, variant C possessing neither capsule nor pili and strain K-12 of Escherichia coli possessing pili alone, and the following results were obtained.
Variants A' and C and strain K-12 were capable of agglutinating the erythrocytes either on the slide glass within 10min or in the test tube within 2 hours, however, the parent strain agglutinated the erythrocytes only in the test tube in more than 10min. This erythrocytes agglutination by strain K-12 and mutant C was inhibited with 0.01% and 10% mannose, respectively, while no such inhibition was observed with variants A' and the parent strain. When the erythrocytes-bacteria aggregate was observed by scanning electronmicroscopy stringlike appendages were observed on the cell surfaces between the erythrocytes and the organisms of the parent strain and mutant A'. However, this was not the case with mutant C or strain K-12. All the mice were killed with 10² and 10⁸ cell of the parent strain and variant A', respectively. No animal was killed even with 10⁸ cells of either variant C or strain K-12, suggesting that the pili act as a pathogenic factor in addition to the capsule.

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A relationship between R plasmids and increased incidence of nonpigmenting drug-resistant Serratia marcescens from clinical sources

Yumiko MUTO, Akiyoshi TSUJI, Yasuko KANEKO, Sachiko GOTO

1982 Volume 37 Issue 6 Pages 931-937
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.931

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We confirmed that nonpigmenting strains of Serratia marcescens were better recipient of R plasmid pMT1 than the pigmenting strains isolated from various clinical sources. Further, R plasmids N-3, R446b, S-a, R471a and R1 (incompatibility groups N, M, W, L, and F11, respectively) were transferred more frequently to a nonpigmenting strain No.1C1 than to a pigmenting strain No.1-1. On the other hand, R plasmids R16 and R6K (incompatibility groups O and X, respectively) were transferred to neither the pigmenting strain No.1-1 nor the nonpigmenting strain No.1C1.
Transfer of R plasmids N-3, R446b R471a or R1 from E. coil J53 to the pigmenting strain No.1-1 caused spontaneous loss of the pigment producibility at a relatively high frequency, whereas such a spontaneous loss of the pigment producibility was not observed in the culture of the parent strain possessing no Rplasmid. On the other hand, transfer of R plasmid S-a did not cause such a spontaneous loss of the pigment producibility.
The above results may explain why so many nonpigmenting, drug-resistant strains of S. marcescens are isolated frequently from clinical sources.

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Morphological Difference between Campylobacter jejuni and C, fetus

Tatsuya MOROOKA, Akiko UMEDA, Kazunobu AMAKO

1982 Volume 37 Issue 6 Pages 939-941
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.939

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A new method of the continuative collection of blood samples and drug injection by rat and mouse jugular veins

Choshun KANETAKE

1982 Volume 37 Issue 6 Pages 943-947
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.943

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[title in Japanese]

1982 Volume 37 Issue 6 Pages 949-955
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.949

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[title in Japanese]

1982 Volume 37 Issue 6 Pages 956-972
Published: November 25, 1982
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3412/jsb.37.956

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