Nippon Saikingaku Zasshi
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
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Volume 47, Issue 4
Displaying 1-4 of 4 articles from this issue
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  • Salmonella vaccines and immunity
    Eiji KITA
    1992 Volume 47 Issue 4 Pages 599-606
    Published: July 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.599
    JOURNAL FREE ACCESS
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  • Detection and identification of pathogenic bacteria by polymerase chain reaction with primers from DNA sequence of ribosomal RNA
    Naoto SAWADA, Yukio IWAMURA, Tohru SHIMIZU, Hideo HAYASHI
    1992 Volume 47 Issue 4 Pages 607-616
    Published: July 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.607
    JOURNAL FREE ACCESS
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    Applicability of the polymerase chain reaction method for identification of pathogenic bacteria was examined with the primers synthesized from the ribosomal RNA gene sequence containing both homologous and species-specific regions of bacterial species from Mycoplasma to Mycobacteria.
    Two out of the nine sets of promoters prepared, each covering about 650 nucleotides spanning from 16S RNA to 23S RNA regions, produced the corresponding DNA fragments from all the strains tested, and another set did so from all species but Mycoplasma. This method enabled one to detect and identify E. coli in a sample containing 2×102 CFU.
    The restriction enzyme patterns of the PCR products obtained with Hae-III, Hha-I, Mbo-I, Msp-I, Rsa-I and Taq-I were so characteristic as to differentiate one species from another. Ten strains of E. coli showed identical restriction patterns and 10 of S. aureus also showed identical patterns indicating that the restriction pattern is species-specific. The method may be applicable to detection and identification of a certain species bacteria which are suspected to be consealed in water or food samples, or clinical specimens, especially when the consealed bacterial genus or species can not be predicted.
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  • Phosphoenolpyruvate
    hexose phosphotransferase systems in Lactobacillus species
    Hiroshi NAGASAKI, Kazuyo ITO, Shigenobu MATSUZAKI, Shuji TANAKA
    1992 Volume 47 Issue 4 Pages 617-624
    Published: July 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.617
    JOURNAL FREE ACCESS
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    The substrate range of phosphoenolpyruvate: hexose phosphotransferase systems (hexose-PTSs) in Lactobacillus casei subsp. casei LAC3 and L. acidophilus LAC5 was examined. Strain LAC3 demonstrated PTS activities for glucose (Glc), mannose (Man), glucosamine (GcN), 2-deoxyglucose (2DG) and fructose (Fru), while strain LAC5 showed the activities only for Man and Fru. These activities were all constitutive. Growth of both strains was strongly inhibited by 2DG. 2DG-resistant mutants DG329 and DG504 were isolated, respectively, from strains LAC3 and LAC5. Mutant DG329 grown on Glc was defective in all the above-described activities observed with strain LAC3, whereas no defect in PTS activities was found in mutant DG504. Mutant DG329, however, showed some inducible activities for Man and Fru when grown on Man, Fru or Scr. These results strongly suggest that strain LAC3 has inducible PTS (s) specific for Man and/or Fru besides the well-known, broadly specific, constitutive Man-PTS, and also that strain LAC5 lacks the Man-PTS, but has other constitutive PTS (s) specific for Man and/or Fru.
    L. fermentum LAC12 had the Man-PTS as reported previously (Nagasaki et al., 1992), but had no inducible activities like those found in strain LAC3.
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  • Effect of pH on preferential Antibacterial-activity of Ethylenediaminetetraacetic acid (EDTA)
    Nori KIDA, Sosuke SUZUKI, Takaaki YAMANAKA, Kazuo FURUYAMA, Fumiaki TA ...
    1992 Volume 47 Issue 4 Pages 625-629
    Published: July 25, 1992
    Released on J-STAGE: February 19, 2009
    DOIhttps://doi.org/10.3412/jsb.47.625
    JOURNAL FREE ACCESS
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    Ethylenediaminetetraacetic acid (EDTA), a chelating agent, was examined for the antibacterial activity against 15 species of bacteria by treating with a 10mM solution at pH adjusted to 5.0, 7.0 or 9.0. All bacterial species tested were classified into three groups; tentatively named the pH 5 EDTA-sensitive group comprising Vibrio cholerae and Staphylococcus aureus, the pH 9 EDTA-sensitive group comprising Escherichia coli and Pseudomonas aeruginosa and the EDTA-nonsensitive group comprising Proteus mirabilis. The EDTA-sensitivity grouping may be used as a tool for preferential decontamination of certain bacteria in live edible fishes, although further experiments are needed to characterize more strains and also species of bacteria.
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