Kanzo
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Volume 27, Issue 9
Displaying 1-23 of 23 articles from this issue
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  • Comparison of hepatitis B virus specific RNA, HBV DNA, and HBV antigenic proteins
    Osamu YOKOSUKA, Masao OMATA, Fumio IMAZEKI, Yoshimi ITO, Junko MORI, K ...
    1986 Volume 27 Issue 9 Pages 1231-1236
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1231
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    Hepatitis B virus RNA and DNA were studied by Northern and Southern blot hybridization method in 30 (17 HBsAg seropositive and 13 HBsAg seronegative) patients with chronic liver disease. HBV RNA was found in all 17 HBsAg seropositive cases, but in none of the 13 HBsAg seronegative cases. Two different species of HBV RNA (21S RNA and 29S RNA) were found. The 21S HBV RNA hybridized with s-gene specific probe and found in all 17 HBsAg seropositive cases. The 29S HBV RNA was found in 9 cases and this RNA hybridized with both s-gene and c-gene specific probe. Of these 9 cases, free HBV DNA was found in 7 cases and HBeAg was seropositive in 8 cases. Hepatic HBsAg was found in all 17 cases with 21S RNA, whereas hepatic HBcAg was found in 6 cases and these cases were all positive for 29S RNA. Thus, the 21S HBV RNA was thought to be the mRNA of HBsAg and the 29S HBV RNA was the viral pregenome RNA and mRNA of HBcAg and HBeAg.
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  • Studies on HBsAg specific and non-specific cellular immune response in HB vaccine inoculated cases and in cases with various liver disease
    Hitoshi TAKAGI, Akira YOSHIKAWA, Masayoshi YAMASHIKI, Toshiaki YOSHIDA ...
    1986 Volume 27 Issue 9 Pages 1237-1243
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1237
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    We investigated the cellular immune response to the HBsAg by the lymphocyte transformation test (LTT) in 40 cases inoculated with HB vaccine and in 46 cases with various liver disease. Vaccinated group had significantly higher stimulation index in LTT with HBsAg than control. In non responders who didn't have anti-HBs in their serum after vaccination of HB vaccine three times, some (5 in 16 cases 31.3%) were positive for LTT with HBsAg. In patients with acute viral hepatitis (type B) 5 in 7 cases (71.4%) became positive in convalescent phase before the elevation of the titer of anti-HBs. Asymptomatic HBsAg carrier didn't respond to the HBsAg at all, although 9 in 20 patients (45%) responded to it in HBsAg positive chronic liver disease.
    In HBsAg carriers, patients with chronic liver disease had low mitogen response, but asymptomatic carriers responded to them equally with normal control. Non responders had no abnormality in mitogen response and lymphocyte subpopulation, so they were supposed to have HBsAg specific defect in antibody formation.
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  • Evolution of HBeAg/anti-HBe system in relation to the histopathological activity of chronic hepatitis type B
    Shuichi WADA
    1986 Volume 27 Issue 9 Pages 1244-1253
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1244
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    Evolution patterns of HBeAg/anti-HBe system in sera of 118 cases with chronic hepatitis type B were studied in relation to the histological characteristics of the biopsied liver.
    Histology of the liver was assessed for its inflammatory activity according to the method by Knodell et al.
    The patients who had seroconversion from HBeAg to anti-HBe revealed liver histology of less activity in periportal necrosis and fibrosis, but of more prominent intralobular degeneration and focal necrosis than did those with persistently positive HBeAg.
    Sequentially biopsied liver histology of the cases with persistent HBeAg showed gradual increase in periportal fibrosis, whereas in seroconverted cases activity indexes in terms of perioportal and intralobular necrosis regressed, but with no significant changes in periportal fibrosis.
    These rusults indicate that patients with chronic type B hepatitis of mild peripotal inflammation and marked intralobular necrosis are much prone to show a seroconversion from HBeAg to anti-HBe and hepatocellular necrosis improves after the seroconversion.
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  • Clinical and electron-microscopical studies on hepatic microstructure in obstructive jaundice
    with special reference to cases whose jaundice was prolonged after biliary decompression
    Tetsuo TAKEUCHI
    1986 Volume 27 Issue 9 Pages 1254-1264
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1254
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    Changes of hepatic microstructure in obstructive jaundice were investigated qualitatively and quantitatively, especially on case whose jaundice was prolonged after biliary decompression, with the following results. With prolonged jaundice, the enlargement and increase of mitochondria (Mt) in hepatic cells and the decrease in the area of cristae membranes were observed. Marked enlargements of the bile canaliculi, marked decrease and shortening of the microvilli and hypertrophy of bile canalicular microfilaments (BCMF) were also found. More remarkable were cases complicated with chronic hepatic diseases and/or cholangitis, accompanying a thinning of the ectoplasm and a decrease of the BCMF. Furthermore, Kupffer cells were hypertrophied and occupied the sinusoid, resulting in circulatory disturbance. In summary, the marked changes of Mt in hepatic cells disturbed energy production and the hypertrophy of Kupffer cells caused circulatory disturbances in sinusoid, leading to decreased hepatic cellular function and delayed recovery after biliary decompression accompanying with disturbances of the bile canaliculi, which caused prolonged jaundice.
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  • Studies on the action mechanism of cholestatic factor: The effect of colchicine on intrahepatic cholestasis induced by cholestatic factor
    Yoshihide SAKAGAMI, Toukan SHIN, Yasuhiro MIZOGUCHI, Keiji MIYAJIMA, Y ...
    1986 Volume 27 Issue 9 Pages 1265-1271
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1265
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    The cholestatic factor, a kind of lymphokines, was produced from the lymphnode cells of tuberculine-sensitized guinea pigs by stimulation with the purified protein derivatives (PPD) in vitro. When this lymphokine (which induces microfilament dysfunction) was injected into the mesenteric vein of a normal rat, a marked reduction of bile flow was induced. Colchicine (a microtubular blocking agent) alone did not affect the bile flow. However, colchicine in combination with cholestatic factor resulted in a greater reduction in bile flow than that seen with cholestatic factor alone. These results support the hypothesis that the "microtubularmicrofilamentous system" is necessary for hepatic bile formation and also suggest that the dysfunction of this system induced by cholestaic factor can produce intrahepatic cholestasis.
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  • Clinical features of primary biliary cirrhosis
    a comparison between symptomatic and asymptomatic patients
    Kiyoshi YAMAZAKI, Kazuyuki SUZUKI, Yoshiaki MIURA, Kazuyoshi ISHIKAWA, ...
    1986 Volume 27 Issue 9 Pages 1272-1280
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1272
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    Twenty-three patients with primary biliary cirrhosis were evaluated for their clinical features, to clarify the differences between symptomatic and asymptomatic patients. Fifteen were symptomatic and 8 were asymptomatic. symptomatic patients had a higher value of serum GOT and γ-GTP (p<0.05) and a higher titer of anti-mitochondrial antibod (p<0.05) than those of asymptomatic patients. Sjögren's syndrome was the most commonly associated disease. Sicca symptom was observed in 55% of all patients and was observed more commonly in symptomatic patients (p<0.05). It was noteworthy that no patients had the positive results for both SS-A and SS-B antibodies, which were known to be specific for Sjögren's syndrome. Although biochemical and immunological data among symptomatic or asymptomatic patients did not differ significantly according to the presence of Sjögren's syndrome, it remains to be determined whether the presence of Sjögren's sysdrome affects the clinical features of primary biliary cirrhosis.
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  • Estimation of risk for hepatocellular carcinoma among HBsAg carriers and heavy drinkers in Japan
    Hideaki TSUKUMA, Isaburo FUJIMOTO, Akira OSHIMA, The Liver Cancer Stu ...
    1986 Volume 27 Issue 9 Pages 1281-1289
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1281
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    Age-specific incidence rates of hepatocellular carcinoma (HCC) in Japan (1980) were estimated by sex, HBsAg status and drinking history, using existing population-based data. Based on them, relative risk and cumulative risk of HCC (30 to 74 years old) were investigated.
    1) The incidence rates of HCC among HBsAg carriers increased exponentially with age from the thirties to the former half of the forties in both sexes. Thereafter the ratio of increase slowed down and ceased.
    2) For male carriers aged 30-44 years, the relative risk for HCC was about one hundred. Such a high relative risk was also shown for female carriers aged 30-49 years.
    3) The cumulative risk of HCC was 20% for male carriers, and 5.6% for female carriers. Meanwhile, it was 1.7% for male non-carriers and 0.45% for female non-carriers.
    4) The male non-carriers' cumulative risk was 5.0% for drinkers and 1.2% for non-drinkers. The male carriers' risk was 40% for drinkers and 16% for non-drinkers.
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  • Production of aminoterminal peptide of type III Procollagen by established human Hepatoma cell lines
    Terumasa HATAHARA, Shogo IGARASHI, Naoya FUNAKI, Hidekazu NAKABAYASHI, ...
    1986 Volume 27 Issue 9 Pages 1290-1295
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1290
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    One cell line from human hepatoma, PLC/PRF/5, was found to produce type III procollagen, while four other ones, huH-1, huH-4, Huh-6c15 and Huh-7, were not. For the assay of aminoterminal peptide of type III procollagen, a radioimmunoassay was used. The productive rate of this peptide by PLC/PRF/5 was one-fortieth of that of human embryonal fibroblasts. The spent media of the above hepatoma cell lines showed stimulative activity on human embryonal fibroblast for collagen synthesis, and the activity remained in the nondialyzable fraction.
    The surrounding tissue composed of fibroblasts might be mainly responcible for the increase of this peptide in the sera of patients with liver cancer.
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  • Confirmation of the presence of lymph channels in hepatoma nodes for use in the treatment of hepatocellular carcinoma
    Hideki SAITSU, Kouji OKUDA, Kouji YOSHIDA, Michiyasu NONAKA, Mitsuharu ...
    1986 Volume 27 Issue 9 Pages 1296-1302
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1296
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    Four patients with hepatocellular carcinoma were injected with an adriamycin-Lipiodol emulsion (ADR-Lip) by direct puncture. In 3 of them, ADR-Lip helped to demonstrate the outline the lymph vessel running form the tumor to the coeliac lymph glands. This was confirmed by plain roentgrams and computed tomograms of the abdomen. Of the 3 patients, 2 were studied by collecting the lymph glands at necropsy or operation. A great number of foreign giant cells surrounding fat droplets of sizes which are considered Lipiodol were observed. Further, the administration of adreamycin at a dose level 3 to 5 times higher than the usual dose caused tumors to be markedly necrotized and reduced in size and alpha-fetoprotein levels to be maintained in a state of equilibration or decreased. Suggestion has been made that this therapy might be of value to patients not tolrating TAE.
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  • Studies on the kinetics of hepatocellular proliferation in regenerating liver
    Takeshi MATSUDA, Shizuo ODASHIMA
    1986 Volume 27 Issue 9 Pages 1303-1312
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1303
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    In order to clarify the growth mechanisms of normal hepatocytes, the kinetics of hepatocellular regeneration after 70% hepatectomy and after hepatic injury induced by Dgalactosamine was studied.
    The liver parenchyma of adult animals is mixed population of cells with differing DNA content. Cytofluorometric analysis revealed that DNA histograms of normal rat liver cells had two peaks in 2c (41%) and 4c (51%) of relative DNA content, and that these cells were arrested in the G0 or G1 phases of the cell cycle. A surgical removal of 70% of the liver in rats induced polyploidization of the hepatocytes, but only slight polyploidization was observed during regeneration after an administration of 1g/kg·bw D-galactosamine. Chromosomal analyses of regenerated livers showed that no hepatocyte had diplochromosomes, thus revealing that the polyploidization of hepatocytes was not due to endoreduplication.
    Moreover, a comparison between the regeneration of the remaining cells after partial hepatectomy and those after D-galactosamine hepatic injury suggests that the growth kinetics of hepatocytes differs depending on the type, grade and strength of stimulation, and that the somatic and focal regulatory mechanisms function in the growth of hepatocytes.
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  • Clinical study on hepatic extraction rate of ICG in patients with portal hypertension
    Kazuhiro TSUKADA
    1986 Volume 27 Issue 9 Pages 1313-1322
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1313
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    Hepatic extraction rate of ICG (ER) was measured by the technique of the hepatic vein catheterisation and single injection method of ICG of 0.5mg per body weight kg in 91 patients with esophageal varices, that consisted of 70 patients with cirrhosis and 21 with idiopathic portal hypertension. All of them underwent the direct interruption surgery.
    ER in cirrhosis (46.3%)was lower than that in idiopathic portal hypertension (60.1%) and that in control (75.9%). ER was correlated with K-ICG, estimated hepatic blood flow, wedged hepatic vein pressure and weight of excised spleen, but not correlated with portal pressure. ER was correlated with several hepatic function tests (A/G, γ-G, ZTT, Ch-e). Moreover ER showed a significant correlation with magnitude of post-operative change of K-ICG level.
    ER was considered useful in determining operative indication and also predicting postoperative prognosis in patients with esophageal varices.
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  • Investigation of portosystemic collaterals by scintiphotosplenoportography
    Masaaki SAITO, Kunihiko OHNISHI, Hideo TANAKA, Masayuki SAITO, Hidetak ...
    1986 Volume 27 Issue 9 Pages 1323-1330
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1323
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    Scintiphotosplenoportography using 99mTc-macroaggregated albumin and ultrasonography were perfomed in 33 patients with chronic liver diseases. In 25 of these patients percutaneous transhepatic portography was also carried out, and in other 8 patients celiac angiography was done.
    Scintiphotosplenoportography was compared with other image diagnoses in the detection of portosystemic collaterals.
    Scintiphotosplenoportography detected the left gastric veins in 75% of the patients, the detection rate being significantly better than 32% by ultrasonography. Scintiphotosplenoportography and ultrasonography were almost the same in the detection of the paraumbilical vein, splello-renal shunts and short gastric veins. Scintiphotosplenoportography was performed before and after percutaneous transhepatic variceal obliteration, or endoscopic variceal sclerotherapy in 7 patients with esophageal varices. The effects of the therapy for varices could be assessed from RI images and changes of hepatic shunt index by scintiphotosplenoportography. Thus, scintiphotosplenoportography proved to be a useful diagnostic tool in the investigation of portosystemic collaterals.
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  • Studies on changes in hilar splenic vein-ear circulation after obliteration of gastroesophageal verices
    Shigeo SUGANO, Yasukiyo SUMINO, Hiroo HOSAKA, Tohru ABEI
    1986 Volume 27 Issue 9 Pages 1331-1335
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1331
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    The changes in hilar splenic vein-ear circulation (S.V-E) was observed after obliteration of gastroesophageal varices in 11 cases with rupture of esophageal varices.
    S.V-E time and concentration change at ear was measured by a bolus injection of indocyanine green (ICG) through hilar splenic vein and the time and concentration was measured by a dye densitogram taken from an ear-piece. The time till the appearance of ICG (AT), the time from AT to the maximum concentration (PT), the sum of AT and PT (CT) and the maximum concentration of the dye at the ear-piece were taken as the indicators.
    The mean AT, PT and CT were significantly prolonged (p<0.01) after obliteration of gastroesophageal varices. In 10 cases hemostases were successful, and in which there were 3 cases who showed prolongations of these three parameters and lower maximum concentrations. In these 3 cases, collateral pathway were obvious only in the vessels which were obliterated. In one case, in which obliteration was not successful, short gastric vein was remainded patent. PT did not change and maximum concentration was increased.
    The changes in the dye densitogram seemed to reflect well the occluded state after obliteration of gastroesophageal varices.
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  • A case with Budd-Chiari syndrome associated with pregnancy
    Kazutoshi YANO, Takatoshi INOUE, Kazuhiro HAYASHIDA, Eisuke YOKOTA, Sh ...
    1986 Volume 27 Issue 9 Pages 1336-1340
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1336
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    We treated a 34-year old woman with Budd-Chiari syndrome which occurred following antepartum hemorrhage. The patient had genital bleeding from a partial placenta previa at the 32nd week of pregnancy and caesarean section was done. Ten days following the operation, her abdomen became extended with massive ascites, which resisted the treatment with diuretics. A peritoneoscopic examination demonstrated a dark brownish-colored liver with an uneven irregular surface. A biopsy specimen disclosed centrizonal dilatation of sinusoids, suggesting of a congestion. Inferior venacavography revealed complete occlusion from the right iliac vein up to the intrahepatic portion of inferior vena cava and the diagnosis of Budd-Chiari syndrome was made. It is presumed that tissue thromboplastin formed as a result of hemorrhage of the partial placenta previa, and cesarean section accelerated the formation of thrombus in the inferior vena cava. She was treated conservatively with diuretics and paracentesis and her recent status is not worsened.
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  • The autopsy case of primary biliary cirrhosis associated with idiopathic thrombocytopenic purpura
    Masuyo NOMURA, Shigeo MATSUKAWA, Toru INAGAKI, Seiji OHTANI, Kohji YAM ...
    1986 Volume 27 Issue 9 Pages 1341-1346
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1341
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    This is the first case of primary biliary cirrhosis (PBC) associated with idiopathic thrombocytopenic purpura(ITP)as far as we examined.
    The patient:57 year-old female, was admitted to our hospital because of liver dysfunction in 1974. The laboratory data revealed serum total birirubin 1.6mg/dl, Al-P 61.1 K.A.U., moderate elevation of transaminase and marked elevation of IgM. Antimitochondrial antibody (AMA) was positive. In 1975, a gingival and nasal bleeding appeared. The platelet count was 2000. Bone marrow examination revealed normocellular marrow and slight increase of megakaryocytes. Treatment with predonisolone was markedly effective for thrombocytopenia and bleeding tendency. She underwent splenectomy and liver wedge biopsy in 1977. The histological findings showed those of CNSDC, compatible with stage I of PBC (Sheuer). Splenectomy was not effective for thrombocytopenia and she was given predonisolone again. On the basis of clinical course and laboratory findings, diagnosis of ITP was established. She died on Nov. 1983. Autopsy showed stage IV of PBC and tuberculous endometritis.
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  • An acidic isozyme of hepatic glutathione S-transferase in malignant tumor patients
    Motonobu SUGIMOTO, Naoya YOSHIDA, Nagaki SHIMADA, Yasushi OGIWARA, Kat ...
    1986 Volume 27 Issue 9 Pages 1347-1348
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1347
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1986 Volume 27 Issue 9 Pages 1349
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1349
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1986 Volume 27 Issue 9 Pages 1350
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1350
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1986 Volume 27 Issue 9 Pages 1351
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1351
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    1986 Volume 27 Issue 9 Pages 1352
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1352
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    1986 Volume 27 Issue 9 Pages 1353
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1353
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1986 Volume 27 Issue 9 Pages 1354
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1354
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1986 Volume 27 Issue 9 Pages 1355-1361
    Published: September 25, 1986
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.27.1355
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