All issues
Volume 33, Issue 5
Displaying 1-10 of 10 articles from this issue
- |<
- <
- 1
- >
- >|
-
Ryotaro MOHRI, Choji KASHIMA, Taketoshi GASHA, Noboru SUGIYAMA1967 Volume 33 Issue 5 Pages 289-293
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe biosynthesis of altenin is discussed in this paper. The altenin toxicity of culture broth appeared and reached to a maximum, after the speed of growth of Alternaria Kikuchiana Tanaka slowed down. The culture broth contained uronic acid, the content of which increased with the period of growth of the cell, and then decreased to a minimum on the eighteenth day, when the toxicity was a maximum. A solution of glucuronic acid did not exhibit the altenin toxicity, but when the cellfree extraction of this fungus was added, the toxicity was observed after one day. From these facts, it seems reasonable to assume that, in this cultivation, uronic acid was produced as an intermediate, and then changed to altenin by the enzyme which is produced in the cell.View full abstractDownload PDF (479K) -
Tokito TOMINAGA1967 Volume 33 Issue 5 Pages 294-300_1
Published: December 30, 1967
Released on J-STAGE: April 03, 2009
JOURNAL FREE ACCESSIn December, 1965, in the warm South-Western part of Japan, an explosive outbreak of unknown plant disease was observed on the fall sown suger beet field, causing a considerable damage. Again in June, 1966, in the cold North-Eastern part, a plant disease suddenly broke out on the spring sown suger beet field, just after the passage of a typhoon. Its symptoms were a little different from those of the former.
In the former case, remarkable streaks were seen on the midlib and petiole, though few ring spots appeared on the leaf-blade. Moreover, the vascular bundles of the root turned black and the crown of the plant, suffering a great damage, was rotten during winter.
In the latter, many ring spots could be seen on the leaf-blade. Some cases of streaks on the midlib and petiole and blackend vascular bundles were observed, but no decay of the crown.
It has been found out that both the diseases were caused by the same pathogen, though there were some differences between them in the time of outbreak, the symptoms on the plant, the rottenness of the crown, etc.
Bacteriological characters of the causal organism resemble those of Pseudomonas aptata or Ps. coronafaciens. Considering, however, the fact that it is pathogenic to beet, nasturtium, and green pepper but not to oats, the auththor cannot but conclude that the pathogen was Ps. aptata (Brown et Jamieson) Stevens.
One of the reasons of the occurrence of the disease, he supposes, was that the beet varieties E-4 (Donyu-2), E-5 (Tsukisappu) etc. are susceptible to the organism. The outbreak of this disease had never been seen in Japan before.
The bacteriological characters of the pathogen are as follows. It is an aerobic, Gram-negative rod, occurring singly, in pairs, with 1 to 2 mono- or bipolar flagella. A green fluorescence is produced in King's agar. On agar the colonies are dirty white, round, convex, smooth, moist, glistning and butyrous. It grows feebly in Uschinsky's solutions, but not in Cohn's. Gelatin is liquefied, but nitrate is not reduced. Milk is neither coagulated nor peptonized, and litmus is reduced. Ammonia is formed but indole and hydrogen sulphide are not produced. Acid but no gas is formed from arabinose, rhamnose, xylose, glucose, fructose, galactose, mannose, sucrose, raffinose, mannitol and sorbitol and, by certain strains, from glycerol also in Ayers, Johnson agar with BTB as indicator; neither acid nor gas from lactose, maltose, starch, inulin, or dextrin or, by certain strains, from glycerol also. Starch is not hydrolysed. It tolerates the addition of 5% sodium chloride solution. The thermal death-point is at 49°C.View full abstractDownload PDF (2381K) -
Satoshi WAKIMOTO1967 Volume 33 Issue 5 Pages 301-310
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSForty one isolates of Xanthomonas citri and forty two isolates of the related phage were obtained from the diseased citrus leaves collected from various citrus areas in Japan.
The phage isolates were classified into 2 groups according to their characteristics. One is a virulent phage named CP1, and the other could be a temperate one named CP2. CP1 phage is the typical one which is widely distributed in Japan. The electronmicroscopic study revealed that CP1 phage has a spherical head of 68mμ in diameter, with a tail of 160×15mμ in length and width. At 55°C after 10min., the phages are almost completely inactivated.
CP1a phage could be a mutant from CP1 and they are distinguishable from one another in the progressive effect of serum albumin and some other proteins on the efficiency of plating. The host range, morphological and serological characteristics, and thermal inactivation point of CP1a are the same as that of CP1.
CP2 phage originally isolated from the culture of the bacterial isolate N6006 produces turbid plaques on the plates seeded with the bacteria presenting a strictly reverse host range from CP1. The particle of CP2 phage consist of a polyhedral head with no visible tail, the diameter of which is 70mμ. The thermal inactivation point of this phage is equal to that of CP1.
The bacterial isolates collected from various localities of Japan were classified accord-ing to phage sensitivity as follows:
1. Sensitive to CP1 but not to CP2…………Lysotype A
2. Sensitive to CP2 but not to CP1…………B
3. Resistant to both CP1 and CP2…………C
Lysotype A and B are widely distributed in Japan. Most of the isolates belonging to A decompose mannit to produce acid. Those belonging to B usually do not, but there are some exceptions.
In the needle inoculation test, not any differentiation in pathogenicity was observed among the isolates according to their localities or source hosts.View full abstractDownload PDF (2024K) -
Hikotoshi TSUMAGARI1967 Volume 33 Issue 5 Pages 311-312
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1043K) -
1967 Volume 33 Issue 5 Pages 313-319
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1308K) -
1967 Volume 33 Issue 5 Pages 320-323
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (761K) -
1967 Volume 33 Issue 5 Pages 324-328
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (952K) -
1967 Volume 33 Issue 5 Pages 328-342
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (2858K) -
1967 Volume 33 Issue 5 Pages 342-348
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1308K) -
1967 Volume 33 Issue 5 Pages 348-353
Published: December 30, 1967
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (1131K)
- |<
- <
- 1
- >
- >|