1. The growth habits of the mycelium on sixteen different media were compared. Among those media used, the apricot decoction and also the apricot decoction agar proved to be the best, being followed by both the agar and liquid media of the dilute Japanese soy with onion decoction and the cornmeal decoction with 1% cane sugar. The aerial mycelium grown on artificial media was almost yellow in color. 2. The relation of temperature to the growth of the fungus in question was studied by growing the mycelium on poured plates of apricot decoction agar, of potato decoction agar and of soy agar, according to SAITO'S formula, incubated at different temperatures. It was found that the fungus in culture grows vigorously at from 24°C. to 32°C. The lowest limit of temperature for its growth seems to lie at a temperature a little higher than 4°C. and the highest limit at from 36°C. to 40°C. 3. By the cultural experiment using BAVENDAMM'S method, the writer classified the fungus as belonging to the group of lignin-dissolving fungi. In the same experiment the best mycelial growth of the fungus was obtained in the cultures containing 0.05-0.1% tannic acid or gallic acid.
1. Puccinia culmicola DIET. Aeciospores of Aecidium Berberidis-Thunbergii P. HEXN. on Berberis Thunbergii DC. var. Maximowiczii FRANCH. et SAV., obtained from three different localities, were inoculated on wheat, but no infection was noted. Telia on Agropyron semicostalum NEES. (Fig. 1) were used to inoculate the barberry. Two weeks after sowing, abundant pycnia developed, being followed by aecia three weeks later (Fig. 2). Remarkably, the mature pycnia smell of putrid fish or glue. Inoculations on the leaves of Agropyron and rye from these aecia produced uredia, quite, identical with those of Puccinia culmicola DIET. On this account and from its morphological resemblance it may be stated that P. culmicola represents a form of P. graminis PERS. 2. Puccinia Zoysiae DIET. Telia of this rust on Zoysia japonica STEUD. were sown on Paederia chinensis HANCE. After twenty-four days accia, to be identified with Aecidium Paederiae DIET. (Fig. 3) were obtained.
A description is given of the intracellular bodies in the leaves of Lilium speciosum f. rubrum affected by mosaic disease. They are usually conspicuous, can be observed in living cells, and are vacuolate and perhaps of plasmic nature; vary considerably in shape and size. They are usually round to ellipsoid, but not commonly very irregular in shape. They range from 6.9 to 34.3μ in width and 8.9 to 68.6μ in length, in many cases larger than the host nuclei themselves. On the seriously affected plant, they are abundantly seen in all epidermal cells of leaves, but in early stage of development of the disease, they are either entirely absent or scanty. In the latter case they are mostly in close contact with the host nuclei, round in shape, without vacuoles, and look like the aggregates of particles. The intracellular bodies are easily stained by several dyes, acidic or basic, of which Heidenhein's iron-alum haematoxylin give most excellent result. When they are treated with 0.5 per cent safranin and then differentiated by N/5 hydrochloric acid, they are stained coral pink and the host nuclei tyrian rose, so that both structures are easily distinguishable from each other. As to the affinity against the dyes the bodies are generally weaker than the nuclei. Treated by osmic acid, the bodies turn yellow but not black after 18 hours in its 1 per cent solution. The MILLON'S reaction is always negative. The bodies remain intact in 15 per cent sulphuric acid for 5 minutes, and are not soluble in alcohol, ether or chloroform. But in N/5 caustic soda solution, they disolve rapidly. The determinations of the hydrogen-ion concentration of these bodies and the host nuclei were made by colorimetric method, as described in detail by SMALL. The result of this experiment has indicated that both are evidently acidic, and their pH lies between almost 4.0 and 4.8.