Using a membrane filter and a selective medium,
Burkholderia plantarii, causal agent of bacterial seedling blight, was detected in farmpond water used in a nursery affected by bacterial seedling blight.
B. plantarii was detected in surface water of the farmpond, but was hardly detected in water at a depth of 2m or at the bottom of the pond. In the summer,
B. plantarii was detected in the farmpond at about 300cfu/100ml at maximum, but in the winter the concentration was less than 5cfu/100ml. In several cases, however, the concentration increased drastically after stirring the water around Gramineae weeds.
B. plantarii was detected from the roots of perennial Gramineae weeds in winter season, suggesting that
B. plantarii may overwinter on Gramineae weeds.
B. plantarii was also detected in every farmpond tested in this area. In addition, application of the farmpond water naturally containing
B. plantarii at about 300 to 750cfu/100ml to seedling boxes caused seedling blight, suggesting that the farmpond water may be an important source of inoculum.
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