Blasticidin S (BcS) and puromycin (PM), inhibitors against protein synthesis in fungi and higher organisms, inhibited tobacco mosaic virus (TMV) multiplication in tobacco leaf-discs, whereas chloramphenicol (CM), inhibitor against bacterial protein synthesis, did not inhibit. TMV produced by antibiotics treatment had the same infectivity as that produced by untreatment. BcS and PM inhibited
14C-leucine incorporation into the cytoplasmic protein of tobacco leaf-discs, but CM did not. CM reduced more rapidly the chlorophyll content in leaf-discs than BcS and PM.
14CO
2-incorporation into infected leaf-discs was strongly inhibited by CM, but not by BcS and PM. Although, under BcS and PM treatments, the rate of
14C-amino acids and
14CO
2-incorporations into TMV was in proportion to the amount of TMV produced, the rate of the former incorporation rather increased by CM under both light and dark conditions and that of
14CO
2-incorporation was decreased, whereas the amount of TMV was not altered by CM. Most of
14CO
2 incorporated into TMV located in TMV protein. Site of TMV synthesis in tobacco leaf-discs and the role of photosynthetic activity of chloroplasts are discussed.
View full abstract