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Volume 50 (1984)
- Issue 5 Pages 565-
- Issue 4 Pages 455-
- Issue 3 Pages 295-
- Issue 2 Pages 149-
- Issue 1 Pages 1-
Volume 50, Issue 3
Displaying 1-18 of 18 articles from this issue
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Shoji YOSHIMURA1984 Volume 50 Issue 3 Pages 295-300
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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Hajimu KOMADA1984 Volume 50 Issue 3 Pages 301-303
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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Tomio YAMAGUCHI1984 Volume 50 Issue 3 Pages 304-306
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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Akira OGOSHI1984 Volume 50 Issue 3 Pages 307-309
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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Hiroshi HAGIWARA1984 Volume 50 Issue 3 Pages 310
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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Tomohide NATSUAKI1984 Volume 50 Issue 3 Pages 311
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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Kaoru Hanada1984 Volume 50 Issue 3 Pages 312
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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Seung Hun YU, Syoyo NISHIMURA, Takayuki HIROSAWA1984 Volume 50 Issue 3 Pages 313-321
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSMorphological variation of the conidia of Alternaria panax isolated from Panax schinseng and its pathogenicity were studied. The conidia of the isolates formed in natural habitats usually had long beaks and were more uniform in size than those produced in vitro. In aging or axenic growth, excessive cellular swelling was recognized characteristically in the fungus. Surface structure of the conidia and chain formation also varied with the culture media and the age of the colony. Based on the review of the literature and the result of the experiments, it is concluded that the description of the fungus described by Nakata and Takimoto in Korea and Japan does not coincide with A. panax. The isolates of A. panax incited foliar diseases not only in P. schinseng but also in other members of the Araliaceae tested. On the leaves of P. schinseng, Aralia elata and Schefflera arboricola, severe leaf spots and blights developed after artificial inoculation, but on the leaves of Fatsia japonica, symptoms were produced only around the wounded part and slowly enlarged. A. elata and F. japonica were found to be new hosts of the fungus.View full abstractDownload PDF (1841K) -
IV. Genetic Relatedness within AG-4Shiro KUNINAGA, Ryozo YOKOSAWA1984 Volume 50 Issue 3 Pages 322-330
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDNA base sequence homology among AG-4 isolates of Rhizoctonia solani was determined by DNA-DNA reassociation kinetics. Six of 14 isolates examined shared high homology ranging from 88.5 to 92.6% [AG-4 homogeneous group I (HG-I)]. There was also a high homology among the remaining 8 isolates, ranging from 88.7 to 101.3% [AG-4 homogeneous group II (HG-II)]. Relatively low values (30.9 to 47.9%) of DNA homology were found when the above two groups were compared. The genetic divergence between the two groups was also clearly demonstrated by the electrophoretic patterns of proteins and cultural appearances. These results indicate that AG-4 involved two types of isolates that are genetically divergent.View full abstractDownload PDF (1324K) -
Ichiro UYEDA, Teruo SANO, Eishiro SHIKATA1984 Volume 50 Issue 3 Pages 331-388
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSCucumber pale fruit viroid was purified from infected cucumber leaves and stems. Nucleic acids were extracted from the frozen tissue by phenol-CHCl3-SDS, and precipitated with ethanol. Polysaccharides were removed by ethylene glycol monomethyl ether treatment and phenolic substrances by cetyltrimethyl ammonium bromide. After 2M LiCl soluble fraction was treated with DNase, low molecular weight RNAs were obtained. The viroid was further purified by CF-11 cellulose chromatography and 15% polyacrylamide gel electrophoresis. Yield of the purified viroid was about 3-6μg/200g of tissue. Five percent polyacrylamide gel electrophoresis of the purified viroid in the presence of urea revealed two bands both associated with infectivity.View full abstractDownload PDF (1505K) -
Teruo SANO, Ichiro UYEDA, Eishiro SHIKATA1984 Volume 50 Issue 3 Pages 339-345
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSHop stunt viroid (HSV), cucumber pale fruit viroid (CPFV), and potato spindle tuber viroid (PSTV) were co-electrophoresed in polyacrylamide gel (PAGE) under both non-denaturing and denaturing conditions. On our experimental condition, they migrated at almost the same rate in 15% PAGE of Tris-acetate-EDTA buffer (non-denaturing condition). Under the 7M urea-denaturing condition, the viroids migrated differently in 5% or 7.5% PAGE of Tris-borate-EDTA buffer. The circular form of HSV and CPFV migrated apparently faster than that of PSTV. The circular form of HSV migrated slightly faster than that of CPFV. A linear form of HSV and CPFV were not detected by staining of the gel but its presence was indicated by infectivity assay. It was difficult to distinguish HSV and CPFV by electron microscopic examination. Mean length of HSV-and CPFV-circular molecules were 83±2.4nm and 82±4.2nm, respectively. The linear form was scarcely observed in both HSV and CPFV preparations.View full abstractDownload PDF (1065K) -
V. Genetic Relatedness within AG-6Shiro KUNINAGA, Ryozo YOKOSAWA1984 Volume 50 Issue 3 Pages 346-352
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDNA base sequence homology among AG-6 isolates of Rhizoctonia solani was determined by DNA-DNA reassociation kinetics. Five of 10 isolates examined shared high DNA homology ranging from 91.8 to 97.7%. This group was classified as AG-6 homogeneous group I (HG-I). DNA homology among the remaining 5 isolates was 55.4 to 66.2%. They showed only 47.5 to 62.9% DNA homology with AG-6 HG-I isolates. These isolates were designated AG-6 genotypic variation (GV) isolates. Each of them appeared to have gene structures that differed by nucleotide substitutions. The genetic divergence among AG-6 isolates was also clearly demonstrated by the electrophoretic patterns of proteins and appearances of the cultures. These results suggest that AG-6 consists of strains that are phylogenetically related but have significant genetic polymorphism.View full abstractDownload PDF (1150K) -
III. Resistance Expression and Loss of Capability for Plasmolysis in Inner Epidermal Cells of Leaf-SheathHironori KOGA, Osamu HORINO1984 Volume 50 Issue 3 Pages 353-360
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSLight and electron microscopic studies were carried out to clarify the relationships be tween cytoplasmic granulation and hypersensitive cell death in the inner epidermal cells of rice leaf-sheaths inoculated with an incompatible race of Pyricularia oryzae Cav. Hypersensitive cell death, as determined by the loss of capability for plasmolysis, was clearly observed by electron microscopy when the epidermal tissues were treated with a hypertonic sucrose solution. In the incompatible combination between race 037 and var. Toride 1 (resistance gene Pi-zt), fragmentation of plasma membranes and destruction of the unit membrane structure were observed in infected epidermal cells that did not plasmolyze. Disruption of the plasma membrane was also observed in non-plasmolyzed cells, suggesting that membrane disruption was not caused by the plasmolysis treatment. Infected epidermal cells in the incompatible combination were found to be alive until invagination and lomasome-like structures were formed in plasma membranes. In the compatible combination between race 037 and var. Koshihikari, ultrastructural changes were not observed in plasma membranes of infected epidermal cells and normal plasmolysis occurred. Thus, it is suggested that hypersensitive cell death may occur when plasma membranes invaginate and form lomasome-like structures then followed by disruption into vesicles which are observed light-microscopically as cytoplasmic granules.View full abstractDownload PDF (2259K) -
Kaoru HANADA1984 Volume 50 Issue 3 Pages 361-367
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSPurified particles of fourteen cucumovirus isolates were analyzed by polyacrylamide gel electrophoresis. Five isolates of cucumber mosaic virus (CMV), five of soybean stunt virus (SSV), three of peanut stunt virus (PSV) and one of chrysanthemum mild mottle virus (CMMV) were used. Each isolate gave only one main peak. The five CMV isolates had the same particle mobility. SSV, PSV and CMMV isolates had mobilities which were different from one another and from CMV. Differences were also found between isolates of PSV and SSV. The usefulness of electrophoretic analysis of virus particles for virus detection and identification is discussed.View full abstractDownload PDF (653K) -
Eishiro SHIKATA, Shinji KAWANO, Toshihiro SENBOKU, Emmanuel R. TIONGCO ...1984 Volume 50 Issue 3 Pages 368-374
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe spherical virus-like particles, 35nm in diameter, were isolated from the rice plants infected with rice ragged stunt, dwarf, black-streaked dwarf, grassy stunt viruses, as well as from “healthy” plants which were not inoculated with those viruses. The size and shape of the particles were also found in the dip preparations of the rice tarsonemid mites and eggs, Steneotarsonemus spinki Smiley (Acarina, Tarsonemidae). The mites were parasitic on the inside surface of the rice leaf sheaths, associating with brown discoloration. Antiserum against the 35nm particles purified from the sheaths after carefully removing the mites and eggs by brushing, reacted with the particles appeared in the dip preparations from the mites and eggs. The purified preparations of the 35nm particles showed typical absorption profile of nucleoprotein.
The particles were detected by ELISA in the leaf sheaths and in the hulls of rice grains, but not in the leaves, roots and paddy water. Ultrathin sections of the leaf sheaths revealed large amount of the 35nm particles in the inner epidermis and the adjacent parenchymatous cells. This is the first report of the virus-like particles associated with the tarsonemid mites that seems to propagate in the rice plants.View full abstractDownload PDF (1308K) -
IV. The Interface between Invading Hyphae and Host Cytoplasm in Epidermal Cells of Leaf-SheathHironori KOGA, Osamu HORINO1984 Volume 50 Issue 3 Pages 375-378
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe fine structure of invading hyphae of Pyricularia oryzae Cav. and invaded inner epidermal cells of rice leaf-sheath, with a compatible combination of race 037 and var. Koshihikari, is observed. The cell wall of the invading hyphae was directly in contact with the cytoplasm of the epidermal cells, suggesting that the invading hyphae penetrated the plasma membrane of the host. Fibrillar structures were occasionally observed at the surface of the cell wall of the invading hyphae.View full abstractDownload PDF (1022K) -
Nobuaki MATSUYAMA, Satoshi WAKIMOTO1984 Volume 50 Issue 3 Pages 379-382
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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1984 Volume 50 Issue 3 Pages 383-446
Published: July 25, 1984
Released on J-STAGE: February 19, 2009
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