Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
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Volume 88, Issue 2
Displaying 1-4 of 4 articles from this issue
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  • Quantitative PCR to determine density of Ceratocystis ficicola and understand resistance of BC1 line Reikodai 1 go derived from hybridization between common fig, Ficus carica, and wild fig, F. erecta
    N. SHIRAKAMI, T. MORITA, S. JIKUMARU, S. SUGAWA, H. YAKUSHIJI
    2022 Volume 88 Issue 2 Pages 91-97
    Published: May 25, 2022
    Released on J-STAGE: May 31, 2022
    DOIhttps://doi.org/10.3186/jjphytopath.88.91
    JOURNAL FREE ACCESS
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    BC1 line Reikodai 1 go, derived from interspecific hybridization of Ficus carica and F. erecta, has resistance to Ceratocystis canker and thus is expected to be used as a new rootstock for fig trees. As a first step in elucidating the resistance mechanism, we used qPCR to quantify the density of Ceratocystis ficicola, the causal agent of Ceratocystis canker, in cuttings of Reikodai 1 go and in susceptible common fig variety Houraishi over time after needle-wound inoculation. Between 1 and 180 days after inoculation, samples were collected at the inoculation site and 2 and 4 cm above it. The amount of DNA of C. ficicola was limited in Reikodai 1 go cuttings relative to that in Houraishi. By 29 days, all Houraishi cuttings were dead, but all Reikodai 1 go cuttings were still alive after 180 days. These results indicate that the resistance of Reikodai 1 go prevents the migration and propagation of the C. ficicola pathogen.

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  • A method to mechanically inoculate tomato plants with tomato chlorosis virus
    S. KIMURA
    2022 Volume 88 Issue 2 Pages 98-104
    Published: May 25, 2022
    Released on J-STAGE: May 31, 2022
    DOIhttps://doi.org/10.3186/jjphytopath.88.98
    JOURNAL FREE ACCESS
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    In 2021, yellowing disease of tomatoes caused by tomato chlorosis virus (ToCV, a crinivirus) was found in two production areas in Kyoto Prefecture, Japan. The disease is emerging as a serious problem for field and greenhouse growers. The virus is known to be transmitted by whiteflies (Bemisia spp. and Trialurodes vaporariorum) and has been assumed to not be mechanically transmissible. For developing management methods for diseases caused by whitefly-transmitted viruses, a mechanical inoculation method is desirable. Here we thus developed a method for mechanical inoculation using a toothbrush that had been dipped in an extract of leaves ground in 4 volumes of 100 mM potassium phosphate buffer (pH 7.0) containing 0.1% (v/v) 2-mercaptoethanol. After 17 days, characteristic symptoms appeared on tomato plants, and ToCV was confirmed in leaf tissue using a one-step reverse transcription-polymerase chain reaction. Transmission efficiency using the method was 81.0%. This finding is the first report of sap transmission for ToCV.

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  • Control efficacy of mixing difenoconazole with protectant fungicides against Japanese pear scab
    Y. IDE, Y. TOMITA, T. OTANI, E. MIYAZAKI, N. TASHIRO
    2022 Volume 88 Issue 2 Pages 105-114
    Published: May 25, 2022
    Released on J-STAGE: May 31, 2022
    DOIhttps://doi.org/10.3186/jjphytopath.88.105
    JOURNAL FREE ACCESS
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    Control efficacy of difenoconazole mixed with protectant fungicides (oxine-copper, ziram-thiram, and iminoctadine albesilate) against Japanese pear scab was evaluated using a meta-analysis. Based on the result of tests conducted in different areas and fields over multiple years, the efficacy of a mixture with oxine-copper or ziram-thiram was inferior to that of difenoconazole alone. On the other hand, the efficacy of difenoconazole mixed with iminoctadine albesilate was superior to that of difenoconazole alone.

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