Japanese Journal of Phytopathology

Studies on the Infection and the Movement of Plant Viruses

Tadao MISAWA

1973 Volume 39 Issue 3 Pages 181-183
Published: June 01, 1973
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.181

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Ecology of Soil-borne Pathogenic Fungi

Tadao UI

1973 Volume 39 Issue 3 Pages 184-185
Published: June 01, 1973
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.184

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[title in Japanese]

1973 Volume 39 Issue 3 Pages 186-245
Published: June 01, 1973
Released on J-STAGE: June 03, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.186

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Effect of Thiophanate-methyl on the Infection Process of Some Plant Pathogenic Fungi

Yasushi YASUDA, Akira NAKATA, Teruhisa NOGUCHI

1973 Volume 39 Issue 3 Pages 246-250_2
Published: June 01, 1973
Released on J-STAGE: April 03, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.246

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Effect of a new fungicide, thiophanate-methyl (Th-M) on the infection process of three fungi, Pyricularia oryzae, Colletotrichum lagenarium and Sphaerotheca fuliginea was investigated microscopically.
Th-M inhibited almost completely the development of hyphae of P. oryzae and C. lagenarium in host cells, whereas spore germination and appressorium formation were scarcely inhibited on the host treated with 500ppm Th-M. The growth of germ tube of S. fuliginea spore was also inhibited on 50ppm Th-M-treated cucumber leaves. Moreover, the swelling or breakage of spore and the distortion of germ tube of P. oryzae spore when directly contacted with 500ppm Th-M. On the other hand, the rice leaf sheath cells treated with Th-M revealed normal convex plasmolysis and the epidermal cell wall of treated cucumber leaves never showed morphological abnormality.
Methyl 2-benzimidazolecarbamate (MBC), a metabolite of Th-M also inhibited the development of invaded hyphae, but no difference between the effects of Th-M and MBC was observed in the infection process. It could be concluded that these acted characteristically rather in the process of penetration than in spore germination and appressorium formation on host cells.

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RNA Polymerase Activity in Purified Rice Dwarf Virus

Tadashi KODAMA, Naoji SUZUKI

1973 Volume 39 Issue 3 Pages 251-258
Published: June 01, 1973
Released on J-STAGE: April 03, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.251

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Purified rice dwarf virus (RDV) particles catalyze incorporation of H³-UMP from H³-UTP into an acid-insoluble polynucleotide product. Optimal synthesis of the polynucleotide occurs at 35C and within a pH range of 8.5-9.0. The H³-UMP incorporation is strictly dependent on all other three ribonucleoside triphosphates and Mg⁺⁺ ion, and is partially dependent on energy regenerating system. The reaction is stimulated slightly by chymotrypsin, not inhibited by actinomycin D or DNase, and strongly inhibited by RNase or pyrophosphate. The reaction proceeds for at least two hours without any detectable lag and is directly proportional to the amount of RDV particles. The polymerase activity cosediments with intact RDV particles in sucrose density gradient and little activity is associated with the empty shells of RDV. The reaction product is RNase-sensitive and is specifically hybridized with heat-denatured, unlabeled RDV-RNA but not with heat-denatured RNA from cytoplasmic polyhedrosis virus of silkworm.

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Effects of Some Chemicals on the Multiplication of Cauliflower Mosaic Virus

Nobuo TEZUKA, Takeshi TANIGUCHI

1973 Volume 39 Issue 3 Pages 259-265
Published: June 01, 1973
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.259

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The effects of a variety of chemicals on the multiplication of cauliflower mosaic virus (CaMV) were investigated by measuring the incorporation rate of ³²P into virus particles in infected Brassica perviridis leaves. Treatment with actinomycin D, 5-bromodeoxyuridine, 5-fluorodeoxyuridine and formycin B inhibited the multiplication of CaMV 20 days after inoculation, but not with 2-thiouracil and blasticidin S. The effects of the above chemicals on the multiplication of CaMV are discussed.

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Studies on Crown Gall

II. On the pigments produced in gall tissue

Ryoichi NISHIZAWA

1973 Volume 39 Issue 3 Pages 266-268_1
Published: June 01, 1973
Released on J-STAGE: April 03, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.266

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The pigments of galls in Sedum alboroseum and Bryophyllum daigremontianum inoculated with Agrobacterium tumefaciens were detected histochemically and spectrophotometrically. Carotenoid, anthocyan and chlorophyll were detected with both methods and flavonoid was only detected histochemically.

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Infectivity of Potato Leaf-roll Virus in Salivary Secretions from Viruliferous Aphids

Satoshi TANAKA, Hiroyuki SHIOTA

1973 Volume 39 Issue 3 Pages 269-271
Published: June 01, 1973
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.269

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Serological Relationship among Chrysanthemum Mild Mottle Virus, Tomato Aspermy Virus and Cucumber Mosaic Virus

Hiroshi TOCHIHARA

1973 Volume 39 Issue 3 Pages 272-274
Published: June 01, 1973
Released on J-STAGE: February 19, 2009

DOIhttps://doi.org/10.3186/jjphytopath.39.272

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