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Volume 45 (1979)
- Issue 5 Pages 591-
- Issue 4 Pages 415-
- Issue 3 Pages 283-
- Issue 2 Pages 135-
- Issue 1 Pages 1-
Volume 45, Issue 2
Displaying 1-23 of 23 articles from this issue
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Takeshi TANIGUCHI, Takao GOTO1979 Volume 45 Issue 2 Pages 135-141
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA purification procedure was devised for obtaining another inhibitor of virus infection than that reported previously occurring in Chenopodium amaranticolor. Frozen leaves were homogenized with neutral buffer and expressed through gauze. The expressed juice was centrifuged at a low speed. The supernatant was brought to 50% saturation with solid ammonium sulfate added slowly and centrifuged. The supernatant was then brought to 100% saturation with solid ammonium sulfate and centrifuged. The inhibitor was further purified from the obtained precipitate by Sephadex G100 and G50 gel filtration and DEAE-Sephadex ion exchange column chromatographies. The ultraviolet absorption spectrum of the purified inhibitor solution showed the typical curve of protein with a maximum absorption at about 280nm. The molecular weight of the inhibitor was determined by two physical methods. The protein may consist of a single polypeptide chain of an approximate molecular weight of 32, 000. The value of this inhibitor was larger than that of the inhibitor which had been obtained from the precipitate with lower concentration of ammonium sulfate13). The electrofocusing profile of the inhibitor showed the presence of two components having isoelectric points at pH 1.8 and 3.7 by using ampholine covering the pH range of 2.5-4.View full abstractDownload PDF (724K) -
Multi-Dish Tray MethodToshihiro SENBOKU, Soon Hyung LEE, Makoto KOJIMA, Eishiro SHIKATA1979 Volume 45 Issue 2 Pages 142-146
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSMicroprecipitin method for serological reaction was modified by using multidish trays instead of petri-dishes. Multi-dish disposo trays (Linbro Chemical Co., Inc.) for tissue culture vessel with 96 holes (φ6mm) made by plastic, were used. Each 0.025ml of antigen and antiserum was mixed by semiautomatic pipettes (Micro selectapette; Clay Adams Co., Inc.). After mixing the mixtures by shaking the trays, paraffin oil (about 0.03ml) was added to prevent drying.
A dark field microscope was used to observe the reaction at magnification 16 or 25. Crude sap or purified preparations of five plant viruses, potato virus X, potato virus Y, potato virus S, cucumber green mottle mosaic virus and rice dwarf virus, were examined. The results indicated that this method was as sensitive as interface ring test. Advantage of this method is that very small amounts of antigen and antiserum are enough to detect the reaction, and one can handle easily many specimens in one tray. It was shown that serological reactions of PVX, PVY, PVS, CGMMV and RDV could be clearly observed and dilution end point of antiserum could be easily determined by this method under a dark field microscope.View full abstractDownload PDF (1110K) -
Reversible Change of Plaque-forming Efficiency in Bdellovibrio by Passage through Different HostsTsutomu UEMATSU, Kan-ichi OHATA1979 Volume 45 Issue 2 Pages 147-155
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSSince plaque-forming efficiency (PFE) of Bdellovibrio isolate BdN6801 was found to vary depending upon the kinds and conditions of indicator bacteria used, the factors causing those variations were studied by using three kinds of bacteria, Erwinia carotovora, Xanthomonas oryzae and Enterobacter aerogenes.
When E. carotovora was used as an indicator, PFE of BdN6801 was markedly influenced by incubation temperature. High titer of PFE was observed on E. carotovora cultured at 20C and the titer was almost the same as that obtained with X. oryzae. Remarkable decrease in PFE was observed when E. carotovora grown at 30C was used. PFE of BdN6801 obtained with E. aerogenes was lower as compared with that with X. oryzae or E. carotovora even under the optimum conditions for plaque formation.
Bdellovibrio BdN6801 obtained from single plaque on X. oryzae was successively cultured on each of the three hosts mentioned above, and the variations in PFE and plaque appearance were examined. Remarkable variation in PFE was not observed in the case of successive transfers on X. oryzae. Conspicuous decrease in PFE was observed on the lawn of X. oryzae when BdN6801 was successively cultured on E. carotovora. When transferred back on X. oryzae, PFE estimated with X. oryzae almost recovered its original level, though plaques were somewhat obscure. On the other hand, when BdN6801 was successively transferred on E. aerogenes, no remarkable change in PFE was observed on the lawn of X. oryzae. However, it produced much clear plaques on the lawn of E. aerogenes as compared with the cells successively transferred on X. oryzae. The results indicate that the variations in PFE and in plaque appearance of Bdellovibrio possibly occur by successive transfers on a single host, and the PFE characterization is reversible.View full abstractDownload PDF (1029K) -
Akira SHIRATA, Kokichi TAKAHASHI1979 Volume 45 Issue 2 Pages 156-161
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSOne-year old shoots of 7 species belonging to 4 genera of Moraceae including mulberry were cut off transversely at the middle part in March in a field, and xylem tissues at the cut ends on the trees were examined in June. All the trees tested showed a brown zone at the part near the cut surface, and acetone extracts from the brownish reaction zone showed antifungal activity against Bipolaris leersiae when tested by the cup method. Acetone extracts from healthy xylem tissues of the same shoots showed no antifungal activity. Cut surfaces of mulberry shoots were inoculated with spore suspension of Fusarium solani f. sp. mori in April. In July, the shoots were cut longitudinally and then new xylem tissue profiles were observed. Both the part near the cut surface as well as the upper part of the lateral shoot, which were several and 10cm respectively distant from the inoculated cut surface, were brownish. The acetone extracts from these two brownish reaction zones showed the antifungal activity; the activity of the former being weak while the later was strong. The hyphae of F. solani f. sp. mori ceased to grow at the part about 1cm above the brown zone near the lateral shoot. Xylem fragments (7×10mm) of one-year old shoots of 7 species of Moraceae sampled in March were treated with water or spore suspension of F. solani f. sp. mori, and incubated for 4 days at 20C. Acetone extracts from inoculated and non-inoculated fragments of all 7 species showed antifungal activity. These antifungal substances were separated into 1 to 3 spots on a TLC sillica gel plate when developed with ethyl ether. The developed TLC plates were sprayed with potato dextrose broth containing spores of B. leersiae and inhibition zones were then observed. The number of inhibition zones and Rf values were almost the same in plants of the same Moraceae genus.View full abstractDownload PDF (1791K) -
Akira SHIRATA, Kokichi TAKAHASHI1979 Volume 45 Issue 2 Pages 162-167
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe production of antifungal substances in xylem tissue of one-year-old mulberry shoots (Morus alba L.) was studied. The xylem blocks (ab. 1cm φ×1cm length) of shoot inoculated with spore suspention of Fusarium solani f. sp mori produced antifungal substances, which were not detected in that of intact shoots. The xylem blocks of uninoculated winter shoot incubated in moist condition also showed the similler effect. These substances seemed to be produced in living tissues, because when the xylem brocks were treated at -60C for 60min or 55C for 10min in water, or chiped too fine it failed to produce the antifungal substances. These substances inhibited the growth of four fungi and two bacteria, the pathogens of mulberry diseases. The antifungal substances were extracted from xylem tissues with methanol, ethanol and acetone but were not extracted with water, ethyl acetate, ethyl ether, chlorofolm and n-hexane. The antifungal substances were separated by silicagel thinlayer chromatography using ethyl ether, and their Rf values of effective spots were 0.10, 0.27 and 0.45. The antifungal substances were briefly concluded as phytoalexins of mulberry.View full abstractDownload PDF (859K) -
Satoshi WAKIMOTO, Yasuko KATSUKI, Norifusa MATSUO, Hiroshi KAMIUNTEN1979 Volume 45 Issue 2 Pages 168-173
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA thread-like structure released from the cell surface of Xanthomonas oryzae isolate N5828 was observed with an electron microscope. Since the centrifuged supernatant of the cell suspension of this bacterium produces plaques on the plates seeded with X. oryzae N5850, the thread-like structure was suspected to be one of the filamentous phages. The plaque forming agent in the centrifuged supernatant of N5828 was propagated by the addition of the susceptible isolate N5850 and was sedimented with 2% polyethylene glycol, followed by low speed centrifugation. The preparation thus obained was further purified by subjecting it to rate-zonal densitygradient centrifugation. The fraction containing plaque forming agent had a UV absorption spectrum typical of nucleoprotein, and there exists close correlation between UV absorption at 260nm and the titer of a plaque forming unit. Electron microscopic observation of the purified preparation reveals thread-like structures of 900-1000nm in length without any contaminants. These results indicate that the thread-like structure released from bacterial cells is one of the filamentous phage.View full abstractDownload PDF (1078K) -
Hiroshi KAMIUNTEN, Satoshi WAKIMOTO1979 Volume 45 Issue 2 Pages 174-181
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSTwo kinds of filamentous phage were found to be released from Xanthomonas oryzae isolates and some of their properties were made clear. The filamentous phage released from X. oryzae N5828 was identified as Xf which was reported by Kuo in 1967, because they were identical in host range, plaque morphology, and serological and morphological properties. The phage released from the bacterial isolate N5845, however, is quite different from Xf. The name proposed is Xf2. The host range of phage Xf2 is very narrow as compared to that of Xf. Neither phage can attack any other xanthomonads tested. The modal length of Xf particles is 965nm, while that of Xf2 is 1573nm. Both phages remained mostly intact after heat treatment at 60C for 60min. Phage Xf is sensitive to chloroform, methyl alcohol and acetone, but not to ethylether, whereas Xf2 is sensitive to ethylether and more sensitive to chloroform than Xf. Twenty-six isolates of X. oryzae out of 50 tested released Xf2 and three released Xf, while one isolate, N5804, released both Xf and Xf2 simultaneously.View full abstractDownload PDF (1063K) -
Yoshitaka TANAKA, Nobuo MURATA, Hajime KATO1979 Volume 45 Issue 2 Pages 182-191
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe nuclear division in developing asci formed by hetrothallic mating within ragistrain of Pyricularia (5 combinations) is compared with that in the asci formed by inter-pathotype mating between either rice-strain (8 combinations) or weeping lovegrass-strain (3 combinations) and ragi-strain. Of the five nuclear divisions, the first two are meiotic, the third is a mitosis in which the four haploid nuclei give rise to eight, and the fourth and the fifth, also mitotic, occur after ascospore delimitation so that the mature spore comprises four uninucleate cells in all cases of mating combinations examined. Lagging chromosomes are observed in meiosis as well as in subsequent mitosis in developing ascus. The number of laggard is one or two per nucleus division. Pooled frequency of laggard-including division in inter-pathotype mating between rice-strain and ragi-strain is 76.7%. That is 13.8% in intra-pathotype mating within ragi-strain and 14.7% in inter-pathotype mating between weeping lovegrass-strain and ragi-strain. In some combinations between rice-strain and ragi-strain, reduced number of ascospores in an ascus, reduced number of cells in an ascospore, and anucleate cells are seen. The haploid number of chromosomes observed through the meiosis and mitoses is six in these strains of Pyricularia.View full abstractDownload PDF (1721K) -
Michiaki IWATA, Yukio SUZUKI, Yasumitsu KONDO, Takeo INOHARA, Tetsuro ...1979 Volume 45 Issue 2 Pages 192-200
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA substance effective against bacterial leaf blight of rice plant was found from fermented broth of Streptomyces zaomyceticus strain SF-1836, by a screening programme employing the direct application via the root system of potted rice seedling. The substance revealed no antibacterial activity upon Xanthomonas oryzae on media, such as potato semi-synthetic agar, which have been used in this screening. In isolation of the substance, therefore, bioassay was carried out by the potted rice seedling test. The active substance was isolated and purified by column chromatgroaphy of ion-exchangers. The substance isolated was an unknown amino acid having molecular formula of C5H7NO2. Later, it was found that all of tested genus Xanthomonas and some other mutants were inhibited its growth on nutritionusly restricted medium such as minimum medium. Its anti-bacterial activity against genus Xanthomonas was counteracted in the presence of L-proline in the minimun basal medium. Therefore the substance may be antagonist of L-proline. On the other hand, in vivo antagonistic effect of L-proline on this substance was not observed.View full abstractDownload PDF (778K) -
Hiroshi YANO, Hiroshi FUJII, Hideo MUKOO, Tsuguaki FUKUYASU, Nobuyuki ...1979 Volume 45 Issue 2 Pages 201-206
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSTransmissibility of dihydrostreptomycin (DHSM) resistance in Pseudomonas lachrymans was studied. Transmission experiments were conducted by mixing-culture method, and it was found that the resistance of 13 P. lachrymans isolates out of 109 DHSM-resistant ones was distinctly transmitted to the recipient isolate of rifampicin-resistant P. lachrymans. Transmission frequency remarkably increased with the lapse of time of mixing-culture, and it was 10-1 at after 24hr. DHSM resistance was not transmitted when culture filtrate of one of the 13 DHSM-resistant isolates was used as donor, suggesting the presence of resistance (R) plasmid in the donor isolate. Several curing agents such as acriflavine, acridine orange etc. were tested against the donor resistant isolate, but the results were negative. From the resistant isolate and R+ transconjugant obtained from it, plasmid DNA was extracted with cleared lysate method and analyzed by agarose gel electrophoresis. From these experiments, it was clarified that the resistant isolate carries at least 2 kinds of plasmid DNA, molecular weights of which were 86×106 and 58×106 daltons. These plasmid DNAs could not be found in the transconjugant. Moreover, transformation experiments by these plasmid DNA samples on DHSM-susceptible P. lachrymans, P. aeruginosa and Escherichia coli were not successful. It seems, therefore, that these 2 kinds of plasmid DNA are unrelated to DHSM resistance, but interpreted as the so called cryptic plasmids.View full abstractDownload PDF (1064K) -
Shiro KUNINAGA, Ryozo YOKOSAWA, Akira OGOSHI1979 Volume 45 Issue 2 Pages 207-214
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSMorphological and physiological properties of AG-6 and AG-BI, new anastomosis groups in R. solani, and anastomosis frequency between AG-BI and other anastomosis groups were investigated.
Metabasidia in AG-6 were barrel-shaped or obovate, 7.5-17.0×5.5-10.0μm and without a median constriction. The length of sterigmata was 4.5-18.8μm and numbers of sterigmata per metabasidium 3-(3.9)-5. Basidiospores were obovate to oblong-ellipsoid, hyaline, smooth, flattened on the inner side, with an apiculus, 5.0-10.0×2.8-5.2μm. They sometimes germinated by the repetition. The mean hyphal width was 8.6μm and the number of nuclei per cell was 3-15. Optimum temperature for growth was 25-30C. Thiamine was autotrophic. Metabasidia in AG-BI were barrel-shaped, short clavate or obovate, 8.8-19.0×7.0-11.3μm and without a median constric-tion. The length of sterigmata was 4.0-12.0μm and numbers of sterigmata per meta-basidium 3-(3.9)-5. Basidiospores were obovate to oblong-ellipsoid, hyaline, smooth, flattened on the inner side, with an apiculus, 5.0-10.0×3.7-6.7μm. They sometimes germinated by the repetition. The mean hyphal width was 10.2μm and the number of nuclei per cell was 5-23. Optimum temperature for growth was 23-25C. Thiamine was heterotrophic. Anastomosis frequency of AG-BI was highest with AG-2-2 and lower by degrees with AG-2-1, AG-3, and AG-6. Judging from their morphological properties of perfect stage, AG-6 and AG-BI are classified as Thanatephorus cucumeris (Frank) Donk.View full abstractDownload PDF (1237K) -
Naotaka FURUICHI, Kohei TOMIYAMA, Noriyuki DOKE, Masayuki NOZUE1979 Volume 45 Issue 2 Pages 215-220
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSLength of time from inoculation to hypersensitive death of infected potato cells can be taken as an indication of the degree of hypersensitive reactivity. The earlier the hypersensitive cell death after inoculation, the higher the hypersensitive reactivity. In this study, observation was made of the time until hypersensitive cell death of potato tuder tissues, which had been inoculated with an incompatible race of Phytophthora infestans at intervals after cutting. The results showed that fresh disks having low or no reactivity attained about half of the maximum hypersensitive reactivity at 8-9hr and the maximum reactivity at about 16-20hr, after cutting. In a second experiment, the disks were treated with 5ppm of blasticidin S (BcS), an inhibitor of protein synthesis, for 15min at various times after cutting, and then all the treated ones were inoculated at the same time after cutting, namely, at 16.5hr. The microscopic observations showed that, the longer the time from cutting to BcS treatment, the earlier the hypersensitive death of the infected cells. It was also observed that the time until cell death reached the maximum when the disks were treated with BcS at 16hr after cutting. Findings indicated that the treatment with BcS can fix and maintain the hypersensitive reactivity to almost the level the cells have attained up to the treatment. The analysis using BcS treatment disclosed that there was a lag of about 4hr before the reactivity began to develop.View full abstractDownload PDF (648K) -
Koushi NISHIYAMA, Tsutomu YAMAMOTO, Manabu UMEKAWA, Akinori EZUKA1979 Volume 45 Issue 2 Pages 221-227
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA bacterial disease of tomato new to Japan was found in Tokushima prefecture in 1976. The bacterium attacks leaf blades and sepals of tomato grown in plastic greenhouse during cold season. It also attacks petioles and stems during warm season in plastic greenhouse as well as in open culture. The symptoms on leaf blades are characterized by irregular black spots of three to four millimeters in size, and those on petioles and stems are dark-green sunken streaks accompanied by internal rot of parenchymatous cells. The bacterial isolates obtained from affected leaf blades, petioles and stems were quite uniform in their bacteriological characteristics. They showed a distinct pathogenicity to tomato and head lettuce by spray inoculation, and to bean, cucumber and eggplant by needle prick inoculation. The bacteriological characteristics and the pathogenicity of this bacterium were identical to those of Pseudomonas viridiflava in the previous descriptions as well as with those of the check isolate of P. viridiflava used in this experiment, SN7647, which was isolated from a cucumber plant affected by marginal blight. From these results, the causal bacterium was identified as Pseudomonas viridiflava (Burkholder 1930) Dowson 1939. This is the first report for this bacterium to attack tomato in Japan.View full abstractDownload PDF (1258K) -
Hiroyuki HIBINO, Nasir SALEH, Martoadmojo ROECHAN1979 Volume 45 Issue 2 Pages 228-239
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSRice ragged stunt causes symptoms distinct from rice grassy stunt: the symptoms are stunting of plants, twisted, ragged, and shortened leaves, branching of tillers, panicle emptiness, and galls along the veins. Virus-like particles of 55-60nm in diameter occurred in dip preparations from the leaf and gall tissues of ragged stunt-diseased rice plants stained with phosphotungstate. Similar particles were found in extracts obtained from infectious vectors Nilaparvata lugens Stal. When the tissues were fixed with glutaraldehyde before dipping, particles had “spikes” around their periphery and 1-6 filaments were extended from the particles. Galls resulted from hyperplasia of phloem tissues and gall cells contained amorphous inclusions visible with the light microscope. Virus-like particles were embedded in viroplasm-like inclusions or were scattered in the cytoplasm of phloem and gall cells. Particles were about 65nm in diameter and had electron dense cores of about 45nm surrounded with less electron dense shells. Thirty to ninety percent of insect vecotrs in infectious colonies contained virus-like particles and about 1/2 to 1/3 of these insects were transmitters. Thin sections of insect transmitters showed that virus-like particles were embedded in viroplasm-like inclusions in the cells of the salivary glands, nerve tissues, muscles, fat bodies, and fore-gut. Particles were abundant and aggregated in crystalline arrangements in salivary glands and fat-body cells. Tubules with virus-like particles occurred in fat-body cells.View full abstractDownload PDF (4419K) -
1. Discovery of New Varietal Groups on the Basis of Reaction Patterns to Five Different Pathotypes of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson in JapanToshiaki YAMADA, Osamu HORINO, Shiro SAMOTO1979 Volume 45 Issue 2 Pages 240-246
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSTo obtain genetic resources of resistance to bacterial leaf blight of rice caused by Xanthomonas oryzae (Uyeda et Ishiyama) Dowson, many foreign and Japanese rice varieties were tested for the resistance to five different pathotypes, groups I, II, III, IV and V, existed in Japan. As the result, most varieties used in the experiment were classified into five varietal groups according to Kozaka's grouping system. Some varieties, however, were not belonged to any of the already-known varietal groups, Kinmaze, Kogyoku, Rantai Emas, Wase Aikoku and Java, because their reaction patterns to five different pathotypes of X. oryzae were different from those of five varietal groups. This paper describes two newly-discovered varietal groups of rice on the basis of their reaction patterns to five different pathotypes of X. oryzae existed in Japan. The isolates of X. oryzae used for the experiment were T7174 for group I, T7147 for group II, T7133 for group III, H75373 for group IV and H75304 for group V. Three weeks after inoculation, scoring was taken about the disease index number according to the standard proposed by Ezuka and Horino. The reaction was considered as S (susceptible) when the value was 2.0 and over, while considered as R (resistant) when the value was below 2.0. One of the newly-discovered varietal groups were S, R, R, S, R to the bacterial groups I, II, III, IV and V, respectively, while the other were S, R, R, S, S in the same way. In the conclusion, the former was termed “Elwee group”, while the latter was termed “Heen Dikwee group”. Three varieties, Elwee, IR 2071-636-5-5 and Dickwee-1, were included in Elwee group, while the other three varieties, Heen Dikwee-1, M104 and M304, were included in Heen Dikwee group. Both genotypes of the newly-discovered varietal groups, Elwee and Heen Dikwee, concerned in those reaction patterns to five different pathotypes of X. oryzae should differ from any one of the already-known five varietal groups. The meanings of the newly-discovered varietal groups were discussed from pathological and breeding points of view in relation to the resistance to bacterial leaf blight of rice.View full abstractDownload PDF (1272K) -
Yuko OHASHI, Toru SHIMOMURA1979 Volume 45 Issue 2 Pages 247-254
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSIt was reported that leaves from hosts which normally become systemically infected with tobacco mosaic virus (TMV) developed necrotic lesions if they were treated with actinomycin D.
The present report describes that ultraviolet light (UV) irradiation induced necrotic lesions on both systemic and local lesion hosts systemically infected with TMV. When leaves of N. glutinosa, Xanthi-nc, or Samsun NN tobacco, inoculated with TMV and incubated at 30C under continuous illumination of 6, 000lux, were irradiated with UV on the upper leaf surface and incubated at light intensities below 200lux, necrotic lesions appeared on the treated leaves. However, no lesions appeared on the treated leaves when UV irradiated leaves were incubated at 6, 000lux. Similar necrotic lesions appeared on TMV-infected leaves of the systemic hosts, Xanthi or Samsun tobacco, by UV irradiation. It was also found that water-soaked lesions developed on the leaves of both kinds of hosts systemically infected with TMV when they were incubated at 30C for more than 10hr in complete darkness, even if infected leaves were not irradiated with UV.
It was apparent that either of treatments of UV irradiation, dark incubation, and actinomycin D which were able to induce necrotic lesions on the leaves systemically infected with TMV, inhibited nucleic acid and protein syntheses more effectively in the cytoplasmic ribosomal fraction of the infected leaves than that in TMV fraction. In the case of UV irradiation followed by incubation at 6, 000lux, and of treatments of formycin B, blasticidin S, and rifampicin all of which were not able to induce necrotic lesions, nucleic acid and protein syntheses were inhibited at the same rate in both TMV and ribosomal fractions. The nature of induction of necrotic lesions on leaves systemically infected with TMV by the various treatments is discussed.View full abstractDownload PDF (2073K) -
Leaf-dip Serology and Multi-dish Tray TestSoon Hyung LEE, Toshihiro SENBOKU, Makoto KOJIMA, Eishiro SHIKATA1979 Volume 45 Issue 2 Pages 255-257
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (728K) -
Hironori KOGA, Hideyoshi TOYODA, Shigeyuki MAYAMA, Jiko SHISHIYAMA1979 Volume 45 Issue 2 Pages 258-260
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (283K) -
Keisuke TOKIMOTO, Mitsuo KOMATSU1979 Volume 45 Issue 2 Pages 261-264
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (346K) -
Yuko OHASHI, Toru SHIMOMURA1979 Volume 45 Issue 2 Pages 265-267
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSDownload PDF (835K) -
Shin'ichi KUSAKARI, Hiromi TSUJI, Kiyoshi YAMADA, Yutaka TANAKA1979 Volume 45 Issue 2 Pages 268-271
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSPyhium sp. was isolated from spinach seedlings, severely affected with damping -off during midsummer. Isolated Pythium sp. grew well at high temperature, and its optimum temperature was 38C. The fungus was morphologically distinguishable from Pythium aphanidermatum in the size of oogonium and antheridium formation. According to the key proposed by Waterhouse (1967), it was identified as Pythium butleri Subramaniam.View full abstractDownload PDF (1039K) -
Hideki NAITO1979 Volume 45 Issue 2 Pages 272-274
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSLeaves of rice variety Sasanishiki were previously inoculated with virulent race N-1 of rice blast fungus. After development of susceptible lesions, the infected leaves of Sasanishiki were reinoculated with avirulent race C-3. Conidia produced on the lesions contained a few conidia of race C-3 pathogenic to variety Fukei 69 or Tachihonami.View full abstractDownload PDF (380K) -
Youichirou KINO, Mikio NOZU, Setsumi ITOI1979 Volume 45 Issue 2 Pages 275-278
Published: April 25, 1979
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSTwelve hours after inoculation, bacterial cells of Agrobacterium tumefaciens were observed at following three sites. (1) Epidermal cells injured by carborundum rubbing (Fig. 1). (2) Intercellular spaces of palisade parenchyma (Fig. 2). (3) Middle lamella of palisade parenchyma (Fig. 3). Forty-eight hours after inoculation, some plant cells in close proximity to A. tumefaciens showed a course of cell division (Fig. 8).View full abstractDownload PDF (1704K)
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