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Volume 7 (1937 - 1938･･･
- Issue 3-4 Pages 163-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Volume 7, Issue 3-4
Displaying 1-10 of 10 articles from this issue
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T. KAWAMURA1938 Volume 7 Issue 3-4 Pages 163-172_2
Published: 1938
Released on J-STAGE: April 03, 2009
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Shigeyasu AKAI1938 Volume 7 Issue 3-4 Pages 173-192
Published: 1938
Released on J-STAGE: April 03, 2009
JOURNAL FREE ACCESSIn this paper the writer has compared the ash figures of leaves of the rice plant grown under a combination-practice of several effective measures for the control of blast disease with those of the same plant cultivated under conventional methods. Regardless of the method of cultivation, the upper portion of leaves surpasses their lower portion in the number of the silicated epidermal cells, and the degree of silicification of the bulliform cells is higher than that of the long and short cells. The number of the silicated epidermal cells per unit area of the leaf, especially of the bulliform cells, is larger in plants grown under such combination-practice than in those cultivated under conventional methods.
Several workers have already pointed out that the blast fungus (Piricularia Oryzae BR. et CAV.) is capable of penetrating the bulliform cells more easily than the long and short cells. There fore, it seems to be very interesting that the number of the silicated bulliform cells varies in accordance with such different practices of cultivation.
The size of the silicated epidermal cells shows no recognizable difference in the plants cultivated under such different practices. However, it is generally recognized that the cells of the upper portion of leaves are wider transversely than those of their lower portion and the cells of the latter elongate longitudinally.View full abstractDownload PDF (2191K) -
Takashi MATSUMOTO, Yasuo HUZIOKA(FUJIOKA)1938 Volume 7 Issue 3-4 Pages 193-202
Published: 1938
Released on J-STAGE: April 03, 2009
JOURNAL FREE ACCESS1. The bacteriophage for Bacterium malvacearum E. F. S. was obtained from the aqueous suspension of the crushed, diseased cotton leaves which had been kept at 10°C for three weeks.
2. The potency of the lytic principle was determined by the following two methods: (1) Dilution method; (2) plaque formation on the potato dextrose agar. The former reveals that the filtrate of three passages has given the titre of 10-7. According to the latter it is found that a loopful of the 1/100 diluted filtrate (the 7th passage) has produced 4 plaques and the same amount of the 1/10 filtrate has given 109. Three drops of the same filtrate seem to be the limit for accurate counting of plaques, as they have given 1950 plaques.
3. The greatest lytic activity may be found near 28°C or between 25°C and 28°C and in all probability the maximum lytic activity approximately parallels the maximum growth of the homologous bacteria. It seems likely that 37°C is near the maximum temperature, and the minimum may come below 10°C.
4. Any of the four strains isolated from the phagic cultures has been proved to be highly resistant to the lytic action. In no case was a strain susceptible to the lytic action recovered. The pathogenicity of these resistant strains, however, has not been appreciably differed from the original, so far as the results of the inoculation on detached cotton bolls are concerned.
5. The size of the plaques produced on the potato dextrose agar varies according to the difference of agar concentration, and enlarges to a certain extent with the increase of the incubation period. In general, the more diluted the agar is, the larger the plaques are.View full abstractDownload PDF (1553K) -
V. INOCULATION STUDIES WITH NATURAL STRAINS, BASIDIOSPORES, SINGLE BASIDIOSPORE ISOLATES, AND SOME F1-, F2- AND BACK CROSS STRAINS OBTAINED BY MATINGKazuo GOTO1938 Volume 7 Issue 3-4 Pages 203-220
Published: 1938
Released on J-STAGE: April 03, 2009
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Influence of Hydrogen Ion Concentration of the Culture Media on the Thermal Death TimeHashio SUZUKI1938 Volume 7 Issue 3-4 Pages 221-230
Published: 1938
Released on J-STAGE: April 03, 2009
JOURNAL FREE ACCESS1. This paper deals with the influence of hydrogen ion concentration of the media, in which the bacteria were cultured, on their thermal death times.
2. The thermal death times were determined by the following method:-The bacteria under experiment were cultured in bouillon nutrient broth, the reaction of which had been adjusted to pH 5.0, 7.0, or 8.6 with either N/1 HCl or NaOH, for 24 hours at 28°C. Test tubes containing 10 cc of the bouillon nutrient broth (reaction of pH 7.0) in which the bacteria were suspended, were placed at different times in a water bath, the temperature of which was electrically controlled at 50±0.5°C, after which the tubes were left to stand for two or three days at 28°C.
Nine strains of Bacillus A, four of Bacillus B, and two of Bacillus C were examined.
3. The thermal death time of Bacillus A, which was cultured in medium having a reaction of pH 5.0, seems to fall between 5 and 10 minutes, excepting that Str. 22, which was isolated from the seed of Ogami, a variety of rice, was killed between 15 and 20 minutes. The thermal death time of Bacillus B, grown in medium showing a reaction of pH 5.0, seems to fall between 10 and 15 minutes, while that of Bacillus C, grown in medium showing the same reaction, seems to lie between 15 and 25 minutes.
4. The thermal death times of the bacteria that were cultured in medium showing a reaction of pH 7.0, seem to fall between 15 and 60 minutes for Bacillus A, between 20 and 25 minutes for Bacillus B, and in more than 60 minutes for Bacillus C.
5. The thermal death times of Bacillus A and B, grown in medium showing a reaction of pH 8.6, seem to fall between 5 and 10 minutes, with an exception in the case of Str. 22 of Bacillus A, which was killed between 10 and 15 minutes, while that of Bacillus C, which was cultured in medium having the same reaction, seems to lie between 10 and 20 minutes.
6. Regardless of species, the thermal death time of bacteria grown in medium with a reaction of pH 7.0 was greater than that grown in medium having a reaction of pH 5.0 or 8.6, and that grown in medium showing a reaction of pH 5.0 was somewhat greater than that grown in medium having a reaction of pH 8.6. Judging from these results, the thermal resistance of bacteria seems to decrease with increase in the hydrogen or hydroxyl ions in the media in which the bacteria are cultured, and the rate of this reduction due to the hydroxyl ion seems to be somewhat larger than that due to the hydrogen ion.View full abstractDownload PDF (499K) -
H. ASUYAMA1938 Volume 7 Issue 3-4 Pages 231-236
Published: 1938
Released on J-STAGE: April 03, 2009
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[in Japanese]1938 Volume 7 Issue 3-4 Pages 237-239
Published: 1938
Released on J-STAGE: April 03, 2009
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[in Japanese]1938 Volume 7 Issue 3-4 Pages 240-248
Published: 1938
Released on J-STAGE: April 03, 2009
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[in Japanese]1938 Volume 7 Issue 3-4 Pages 249-252
Published: 1938
Released on J-STAGE: April 03, 2009
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H. ASUYAMA1938 Volume 7 Issue 3-4 Pages 253-261
Published: 1938
Released on J-STAGE: April 03, 2009
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