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Volume 41 (1975)
- Issue 5 Pages 423-
- Issue 4 Pages 337-
- Issue 3 Pages 233-
- Issue 2 Pages 131-
- Issue 1 Pages 1-
Volume 41, Issue 3
Displaying 1-8 of 8 articles from this issue
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Keiichi TOMARU1975 Volume 41 Issue 3 Pages 233-235
Published: July 25, 1975
Released on J-STAGE: February 19, 2009
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Michio HORI1975 Volume 41 Issue 3 Pages 236-238
Published: July 25, 1975
Released on J-STAGE: February 19, 2009
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Yasuji ASADA1975 Volume 41 Issue 3 Pages 239-241
Published: July 25, 1975
Released on J-STAGE: February 19, 2009
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1975 Volume 41 Issue 3 Pages 242-306
Published: July 25, 1975
Released on J-STAGE: February 19, 2009
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Akira OHUCHI, Tokito TOMINAGA1975 Volume 41 Issue 3 Pages 307-313
Published: July 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSIn order to give a full explanation for tissue degradation phenomenon by pectolytic enzyme of soft rot pseudomonad, endo-PTE, the present investigation was conducted to confirm the molecular sizes and molecular weight distributions of water-soluble polysaccharides (F-I subfraction), which were liberated from cell wall-materials during the macerating action, by viscometric measurement and Sephadex gel-filtration method. In this experiment, 0.2, 0.3 and 0.4M fractions, which were considered as major components of the water-soluble carbohydrates, were mainly used and compared in the degree of polymerization with some pectate markers prepared from pectinic acid according to the methods of Okamoto et al. and Hatanaka et al.
Acid-insoluble pectic acid Ia was used as a standard material, which showed a ratio of uronide residues to aldehyde groups to be 29.96. Assuming that the carbohydates used, are higher molecule of straight chain, mean degrees of polymerization of 0.2, 0.3 and 0.4M fractions were calculated at about 33, 96 and 125 by Ostwald's viscometric measurement, respectively. From these results, it seemed that mean viscometric mole-cular weight differs among each fraction, and about 6×103, 2×104 and 2.5×104, respectively.
By Sephadex G-100 gel-filtration, degree of polymerization of 0.4M fraction was estimated approximately at 50, or more. Carbohydrates of 0.3 and 0.2M fractions were separated into two components by Sephadex gel column, viz. relatively higher and lower molecular compounds. Degrees of polymerization of the higher molecular compounds obtained from both fractions were estimated approximately 50 or more, which were similar to 0.4M fraction. Lower molecular components may be polyuronides, degrees of polymerization of which are assumed to be about 15∼45 in 0.2M fraction, and 30∼40 in 0.3M fraction. It is supposed, therefore, that carbohydrates of 0.2 and 0.3M fractions were the complexes of some components different in molecular sizes, and that these carbohydrates used in this experiment were released from so-called protopectin of cell wall-materials by endo-PTE at random manner.View full abstractDownload PDF (861K) -
Hiroshi OKAZAKI1975 Volume 41 Issue 3 Pages 314-320
Published: July 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSGermination of chlamydospores of Fusarium oxysporum f. raphani was examined on a glass microscope slide coated with collodion film.
The germination rate was slightly decreased at pH8.0, and was dependent on chlamydospore density in a mineral salts solution (BMS). Full dependence of the germination on exogenous glucose concentration was observed at higher densities (106/ml) of chlamydospore suspension.
Inhibition of the germination in lower concentrations of O2 was higher in BMS than in the BMS amended with glucose (BMSG).
Germination of chlamydospores in BMS was inhibited in the atmosphere of the Petri-dish with mycelial suspension of F. oxysporum f. raphani at the bottom. The inhibition was annulled in BMSG. Furthermore, the inhibition was more strong in the starved mycelium than in the non-starved mycelium, and was annulled when glucose was added to the mycelial suspension.
It is discussed that mycelium produces two types of volatile substances, the one of which is inhibitory for germination, the other stimulant. Production of these substances seems to depend upon carbon levels available for the fungus.View full abstractDownload PDF (802K) -
I) Effect of Hymexazol on Cell Permeability of Pellicularia sasakiiToshiro NAKANISHI, Yukiyoshi TAKAHI, Kazuo TOMITA1975 Volume 41 Issue 3 Pages 321-328
Published: July 25, 1975
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe mode of action of hymexazol against Pellicularia sasakii was studied. When the living mycelium of P. sasakii was incubated in aqueous solution of hymexazol for 24hr, a decrease in dry mycelial weight caused. Measurable decrease occurred at 50ppm, with progressively greater weight losses as the concentration increased. On the other hand, a decrease in the dry weight of heat-treated mycelium was negligible even at 1000ppm of hymexazol. The decrease in the dry mycelial weight was found to be closely correlated to a leakage of protein from the mycelium. At 250ppm of hymexazol, the protein leakage increased in almost linear fashion during 8hr incubation period, and then leveled off. When the mycelium was immersed in hymexazol solution for 1hr and subsequently incubated in distilled water for 23hr, a large amouut of protein leaked out from the mycelium during the 23hr incubation period in distilled water, which was in proportion to the concentration of hymexazol. While only a small amount of protein leaked out during the 1hr incubation period in hymexazol solution. Hymexazol inhibited the uptake of glucose by P. sasakii. Hymexazol was readily absorbed by the mycelium from the ambient solution within the first 1hr, and then the absorption reached to equilibrium. On potato-sucrose agar containing hymexazol at 100ppm or 10ppm, the growth of P. sasakii was reduced to some extent. At growth-inhibitory concentration of hymexazol, the hyphae appeared swollen parts as the hyphal tips and at various points along the sides of hyphae, and in certain cases the hyphae burst with loss of protoplasm.View full abstractDownload PDF (1216K) -
1975 Volume 41 Issue 3 Pages 335
Published: 1975
Released on J-STAGE: February 19, 2009
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