Standards of reference range for all laboratory test values are mandatory. This study was designed to establish a reference range for blood vitamin B_1 levels, since the normal range has not been determined in the Japanese population. We found the Japan Committee for Vitamin Laboratory Standards, which was incorporated with the Vitamin Society of Japan and the Japanese Society of Nutrition and Food Science. We standardized whole blood vitamin B_1 levels using three HPLC techniques (post-column revesed-phase HPLC, pre-column reversed-phase HPLC, and pre-column GP-HPLC). The reference range was obtained in 54 volunteers administered a 1800 Kcal diet with 2 mg of vitamin B_1 (1.74 mg measured) daily to avoid marginal vitamin B_1 deficiency in the population. The range for each assay was 26〜47 ng/ml, 28〜51 ng/ml and 28〜56 ng/ml, respectively. Our data suggest that 26〜28 ng/ml is the lower limit of normal for whole blood vitamin B_1, but further studies in a larger population are needed to obtain more definitive results.
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