巻号一覧
50 巻, 11 号
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中島 謙二原稿種別: 本文
1976 年 50 巻 11 号 p. 393-407
発行日: 1976/11/25
公開日: 2018/03/06
ジャーナル フリーPDF形式でダウンロード (1442K) -
照内 淳也, 岡村 正人原稿種別: 本文
1976 年 50 巻 11 号 p. 409-413
発行日: 1976/11/25
公開日: 2018/03/06
ジャーナル フリーThe release of L-ascorbic acid (AsA) and L-dehydroascorbic acid (DAsA) from human erythrocyte was investigated in vitro. The erythrocyte, preincubated with DAsA, was used. It contained DAsA and AsA produced by reduction of DAsA in erythrocyte. AsA was released very slowly into the medium from the erythrocyte to an equilibrium state during 20 hours. The release of DAsA was rapidly and its concentration in the medium became equal to that in the cell within one minute.抄録全体を表示PDF形式でダウンロード (561K) -
富永 文雄, 古野 厚次, 嶋川 幸三, 鈴置 二郎原稿種別: 本文
1976 年 50 巻 11 号 p. 415-420
発行日: 1976/11/25
公開日: 2018/03/06
ジャーナル フリー正常シロネズミにDBT-〔^<14>C〕(outer)を経口投与し, その安息香酸部分の排泄及び尿中代謝物を検索した.投与した安息香酸部分の62〜86%が投与後6時間以内に, また86〜89%が24時間以内に尿中に排泄された.尿中に排泄された^<14>C代謝物の約95%は馬尿酸として同定され, DBTやOBTは検出されなかった.これらの結果はDBTの安息香酸部分はほぼ定量的に馬尿酸として尿中に排泄されることを示している.同様の実験によりCCl_4障害肝又は低タンパク性コリン欠乏による強度の食餌性脂肪肝を持ったシロネズミにおけるDBTの代謝を検索した.尿中ヘの総^<14>C及び馬尿酸の排泄はこれら肝障害シロネズミにおいても対照正常群と変化がなく, DBTの代謝能力にはなんらの低下を認めなかった.抄録全体を表示PDF形式でダウンロード (584K) -
小林 正, 足立 昌子, 吉田 肇子原稿種別: 本文
1976 年 50 巻 11 号 p. 421-429
発行日: 1976/11/25
公開日: 2018/03/06
ジャーナル フリーGas-liquid chromatographic (GLC) determination of vitamin D_2 and ergosterol in Shiitake (Lentinus edodes) was investigated and simple routine methods were established. The method for determination of vitamin D_2 includes alkali-saponification, isolation of the unsaponifiable matters, thin-layer chromatography (TLC), trimethylsilylation and GLC. When contents of vitamin D_2 are very small and comparatively large amounts of sterols exist in a sample, however, digitonin-Celite column chromatography should be added before TLC in order to eliminate the influences of sterols. On the other hands, ergosterol is determined by applying directly the trimethylsilyl ethers of the unsaponifiable matters to GLC. When dry Shiitake were irradiated by the ultraviolet lights of 3 E-Viton/cm^2 (30 μW/cm^2), the relationship between yield of vitamin D_2 and irradiation time was proportional and the yield of vitamin D_2 was 29IU/min. The estimated values of ergosterol in commercial dry Shiitake obtained from different production places were distributed in the range of 0.25〜0.74%, while that in a commercial raw sample was 0.05%.抄録全体を表示PDF形式でダウンロード (966K) -
山田 良平, 辻 孝彦原稿種別: 本文
1976 年 50 巻 11 号 p. 431-435
発行日: 1976/11/25
公開日: 2018/03/06
ジャーナル フリーAminoethyl derivative of Sephadex G-25 specifically adsorbed pyridoxal 5'-phosphate (PALP) among the six vitamin B_6 compounds under appropriate conditions. Optimum pH for the adsorption was around 9,and elution was effected by the use of acids, amino compounds or buffers of high ionic strength. The coloring of PALP adsorbed besides above facts indicated that the adsorption was mainly due to Schiff base formation between PALP and the aminoethyl side chain of Sephadex and also due to anion exchanger function of the same side chain. Isolation of PALP produced by incubation of pyridoxine with Escherichia coli cells was attempted by the use of aminoethyl-Sephadex columns, and a spectrophotometrically pure preparation of PALP was readily obtained.抄録全体を表示PDF形式でダウンロード (503K)
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